Large scale production of liquid wax ester by immobilized lipase
Oleyl oleate, a liquid wax ester was synthesized by an immobilized Candida antartica lipase B (Novozym 435) as biocatalyst using oleic acid and oleyl alcohol. The effects of various reaction parameters were optimized to obtain a high yield of liquid wax esters. Investigation in large scale productio...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Japan Oil Chemists' Society
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7057/ http://psasir.upm.edu.my/id/eprint/7057/1/Large%20scale%20production%20of%20liquid%20wax%20ester%20by%20immobilized%20lipase.pdf |
| _version_ | 1848840490984669184 |
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| author | Mat Radzi, Salina Basri, Mahiran Salleh, Abu Bakar Ariff, Arbakariya Mohammad, Rosfarizan Abdul Rahman, Mohd Basyaruddin Raja Abdul Rahman, Raja Noor Zaliha |
| author_facet | Mat Radzi, Salina Basri, Mahiran Salleh, Abu Bakar Ariff, Arbakariya Mohammad, Rosfarizan Abdul Rahman, Mohd Basyaruddin Raja Abdul Rahman, Raja Noor Zaliha |
| author_sort | Mat Radzi, Salina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Oleyl oleate, a liquid wax ester was synthesized by an immobilized Candida antartica lipase B (Novozym 435) as biocatalyst using oleic acid and oleyl alcohol. The effects of various reaction parameters were optimized to obtain a high yield of liquid wax esters. Investigation in large scale production was performed in batch mode of stirred tank reactor (STR) with one multi-bladed impeller. The optimum condition to produce liquid wax ester was, reaction time (RT); 30 min, temperature (T); 50 °C, amount of enzyme (E); 90 g (900,000 PLU), agitation speed (A) of 400 rpm, number of impeller tip (N) of 2 and molar ratio of oleyl alcohol to oleic acid (M); 2:1. Analysis of the yield showed that at optimum condition, >90% liquid wax esters were produced. The stability of Novozym 435 showed at high percentage (>80%) up to 4 cycles. |
| first_indexed | 2025-11-15T07:28:11Z |
| format | Article |
| id | upm-7057 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:28:11Z |
| publishDate | 2005 |
| publisher | Japan Oil Chemists' Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-70572016-09-28T03:19:16Z http://psasir.upm.edu.my/id/eprint/7057/ Large scale production of liquid wax ester by immobilized lipase Mat Radzi, Salina Basri, Mahiran Salleh, Abu Bakar Ariff, Arbakariya Mohammad, Rosfarizan Abdul Rahman, Mohd Basyaruddin Raja Abdul Rahman, Raja Noor Zaliha Oleyl oleate, a liquid wax ester was synthesized by an immobilized Candida antartica lipase B (Novozym 435) as biocatalyst using oleic acid and oleyl alcohol. The effects of various reaction parameters were optimized to obtain a high yield of liquid wax esters. Investigation in large scale production was performed in batch mode of stirred tank reactor (STR) with one multi-bladed impeller. The optimum condition to produce liquid wax ester was, reaction time (RT); 30 min, temperature (T); 50 °C, amount of enzyme (E); 90 g (900,000 PLU), agitation speed (A) of 400 rpm, number of impeller tip (N) of 2 and molar ratio of oleyl alcohol to oleic acid (M); 2:1. Analysis of the yield showed that at optimum condition, >90% liquid wax esters were produced. The stability of Novozym 435 showed at high percentage (>80%) up to 4 cycles. Japan Oil Chemists' Society 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7057/1/Large%20scale%20production%20of%20liquid%20wax%20ester%20by%20immobilized%20lipase.pdf Mat Radzi, Salina and Basri, Mahiran and Salleh, Abu Bakar and Ariff, Arbakariya and Mohammad, Rosfarizan and Abdul Rahman, Mohd Basyaruddin and Raja Abdul Rahman, Raja Noor Zaliha (2005) Large scale production of liquid wax ester by immobilized lipase. Journal of Oleo Science, 54 (4). pp. 203-209. ISSN 1345-8957; ESSN: 1347-3352 https://www.jstage.jst.go.jp/article/jos/54/4/54_4_203/_article 10.5650/jos.54.203 |
| spellingShingle | Mat Radzi, Salina Basri, Mahiran Salleh, Abu Bakar Ariff, Arbakariya Mohammad, Rosfarizan Abdul Rahman, Mohd Basyaruddin Raja Abdul Rahman, Raja Noor Zaliha Large scale production of liquid wax ester by immobilized lipase |
| title | Large scale production of liquid wax ester by immobilized lipase |
| title_full | Large scale production of liquid wax ester by immobilized lipase |
| title_fullStr | Large scale production of liquid wax ester by immobilized lipase |
| title_full_unstemmed | Large scale production of liquid wax ester by immobilized lipase |
| title_short | Large scale production of liquid wax ester by immobilized lipase |
| title_sort | large scale production of liquid wax ester by immobilized lipase |
| url | http://psasir.upm.edu.my/id/eprint/7057/ http://psasir.upm.edu.my/id/eprint/7057/ http://psasir.upm.edu.my/id/eprint/7057/ http://psasir.upm.edu.my/id/eprint/7057/1/Large%20scale%20production%20of%20liquid%20wax%20ester%20by%20immobilized%20lipase.pdf |