Comparative Evaluation of Three Purification Methods for the Nucleocapsid Protein of Newcastle Disease Virus from Escherichia coli Homogenates.
In the present study, the performances of conventional purification methods, packed bed adsorption (PBA), and expanded bed adsorption (EBA) for the purification of the nucleocapsid protein (NP) of Newcastle disease virus (NDV) from Escherichia coli homogenates were evaluated. The conventional method...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/700/ http://psasir.upm.edu.my/id/eprint/700/1/PFBSB20.pdf |
| _version_ | 1848838842317012992 |
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| author | Yan, Peng Tan Tan, Chuan Ling Yusoff, Khatijah Wen, Siang Tan Beng, Ti Tey |
| author_facet | Yan, Peng Tan Tan, Chuan Ling Yusoff, Khatijah Wen, Siang Tan Beng, Ti Tey |
| author_sort | Yan, Peng Tan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In the present study, the performances of conventional purification methods, packed bed adsorption (PBA), and expanded bed adsorption (EBA) for the purification of the nucleocapsid protein (NP) of Newcastle disease virus (NDV) from Escherichia coli homogenates were evaluated. The conventional methods for the recovery of NP proteins involved multiple steps, such as centrifugation, precipitation, dialysis, and sucrose gradient ultracentrifugation. For the PBA, clarified feedstock was used for column loading, while in EBA, unclarified feedstock was used. Streamline chelating immobilized with Ni^2^+ ion was used as an affinity ligand for both PBA and EBA. The final protein yield obtained in conventional and PBA methods was 1.26% and 5.56%, respectively. It was demonstrated that EBA achieved the highest final protein yield of 9.6% with a purification factor of 7. Additionally, the total processing time of the EBA process has been shortened by 8 times compared to that of the conventional method. |
| first_indexed | 2025-11-15T07:01:59Z |
| format | Article |
| id | upm-700 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:01:59Z |
| publishDate | 2005 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-7002013-05-27T06:50:14Z http://psasir.upm.edu.my/id/eprint/700/ Comparative Evaluation of Three Purification Methods for the Nucleocapsid Protein of Newcastle Disease Virus from Escherichia coli Homogenates. Yan, Peng Tan Tan, Chuan Ling Yusoff, Khatijah Wen, Siang Tan Beng, Ti Tey In the present study, the performances of conventional purification methods, packed bed adsorption (PBA), and expanded bed adsorption (EBA) for the purification of the nucleocapsid protein (NP) of Newcastle disease virus (NDV) from Escherichia coli homogenates were evaluated. The conventional methods for the recovery of NP proteins involved multiple steps, such as centrifugation, precipitation, dialysis, and sucrose gradient ultracentrifugation. For the PBA, clarified feedstock was used for column loading, while in EBA, unclarified feedstock was used. Streamline chelating immobilized with Ni^2^+ ion was used as an affinity ligand for both PBA and EBA. The final protein yield obtained in conventional and PBA methods was 1.26% and 5.56%, respectively. It was demonstrated that EBA achieved the highest final protein yield of 9.6% with a purification factor of 7. Additionally, the total processing time of the EBA process has been shortened by 8 times compared to that of the conventional method. 2005 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/700/1/PFBSB20.pdf Yan, Peng Tan and Tan, Chuan Ling and Yusoff, Khatijah and Wen, Siang Tan and Beng, Ti Tey (2005) Comparative Evaluation of Three Purification Methods for the Nucleocapsid Protein of Newcastle Disease Virus from Escherichia coli Homogenates. The Journal of Microbiology, 43 (3). pp. 295-300. ISSN 1225-8873 English |
| spellingShingle | Yan, Peng Tan Tan, Chuan Ling Yusoff, Khatijah Wen, Siang Tan Beng, Ti Tey Comparative Evaluation of Three Purification Methods for the Nucleocapsid Protein of Newcastle Disease Virus from Escherichia coli Homogenates. |
| title | Comparative Evaluation of Three Purification Methods for the Nucleocapsid Protein of Newcastle Disease Virus from Escherichia coli Homogenates. |
| title_full | Comparative Evaluation of Three Purification Methods for the Nucleocapsid Protein of Newcastle Disease Virus from Escherichia coli Homogenates. |
| title_fullStr | Comparative Evaluation of Three Purification Methods for the Nucleocapsid Protein of Newcastle Disease Virus from Escherichia coli Homogenates. |
| title_full_unstemmed | Comparative Evaluation of Three Purification Methods for the Nucleocapsid Protein of Newcastle Disease Virus from Escherichia coli Homogenates. |
| title_short | Comparative Evaluation of Three Purification Methods for the Nucleocapsid Protein of Newcastle Disease Virus from Escherichia coli Homogenates. |
| title_sort | comparative evaluation of three purification methods for the nucleocapsid protein of newcastle disease virus from escherichia coli homogenates. |
| url | http://psasir.upm.edu.my/id/eprint/700/ http://psasir.upm.edu.my/id/eprint/700/1/PFBSB20.pdf |