Targeting double genes in multiplex PCR for discriminating bovine, buffalo and porcine materials in food chain
Beef, buffalo and pork are the major meat of economic, religious and health concern. Current methods to authenticate these materials in food chain are based on mainly single gene targets which are susceptible to break down by food processing treatments. We, for the first time, described here a doubl...
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| Format: | Article |
| Language: | English |
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Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63382/ http://psasir.upm.edu.my/id/eprint/63382/1/Targeting%20double%20genes%20in%20multiplex%20PCR%20for%20discriminating%20bovine%2C%20buffalo%20and%20porcine%20materials%20in%20food%20chain.pdf |
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| author | Hossain, M. A. Motalib Ali, Md. Eaqub Hamid, Sharifah Bee Abd Asing Mustafa, Shuhaimi Desa, Mohd Nasir Mohd Zaidul, I. S. M. |
| author_facet | Hossain, M. A. Motalib Ali, Md. Eaqub Hamid, Sharifah Bee Abd Asing Mustafa, Shuhaimi Desa, Mohd Nasir Mohd Zaidul, I. S. M. |
| author_sort | Hossain, M. A. Motalib |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Beef, buffalo and pork are the major meat of economic, religious and health concern. Current methods to authenticate these materials in food chain are based on mainly single gene targets which are susceptible to break down by food processing treatments. We, for the first time, described here a double gene targeting short-amplicon length multiplex polymerase chain reaction assay for discriminating bovine, buffalo and porcine materials in a single assay platform. The advantage of the assay is evidenced in terms of fidelity, cost and time since it is highly unlikely that two different targets would be missing even in a decomposed specimen. Detection of multiple targets in a single assay definitely saves analytical cost and time. Mitochondrial cytochrome b (cytb) and ND5 genes were targeted and six different targets (length: 90–146 bp), two for each of cow (120 and 106bp), buffalo (90 and 138bp) and pig (73 and 146bp), were amplified from raw, boiled, autoclaved and microwaved cooked meat under pure and mixed matrices. The detection limit was 0.02 ng DNA under pure states and 0.1% meat in binary mixtures and meatball products. Screening of Malaysian meatball products revealed all beef products were buffalo positive in which 35% were totally replaced. In contrast, all pork products were found uncontaminated from beef and buffalo. |
| first_indexed | 2025-11-15T11:15:13Z |
| format | Article |
| id | upm-63382 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:15:13Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-633822018-10-16T09:08:39Z http://psasir.upm.edu.my/id/eprint/63382/ Targeting double genes in multiplex PCR for discriminating bovine, buffalo and porcine materials in food chain Hossain, M. A. Motalib Ali, Md. Eaqub Hamid, Sharifah Bee Abd Asing Mustafa, Shuhaimi Desa, Mohd Nasir Mohd Zaidul, I. S. M. Beef, buffalo and pork are the major meat of economic, religious and health concern. Current methods to authenticate these materials in food chain are based on mainly single gene targets which are susceptible to break down by food processing treatments. We, for the first time, described here a double gene targeting short-amplicon length multiplex polymerase chain reaction assay for discriminating bovine, buffalo and porcine materials in a single assay platform. The advantage of the assay is evidenced in terms of fidelity, cost and time since it is highly unlikely that two different targets would be missing even in a decomposed specimen. Detection of multiple targets in a single assay definitely saves analytical cost and time. Mitochondrial cytochrome b (cytb) and ND5 genes were targeted and six different targets (length: 90–146 bp), two for each of cow (120 and 106bp), buffalo (90 and 138bp) and pig (73 and 146bp), were amplified from raw, boiled, autoclaved and microwaved cooked meat under pure and mixed matrices. The detection limit was 0.02 ng DNA under pure states and 0.1% meat in binary mixtures and meatball products. Screening of Malaysian meatball products revealed all beef products were buffalo positive in which 35% were totally replaced. In contrast, all pork products were found uncontaminated from beef and buffalo. Elsevier 2017-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63382/1/Targeting%20double%20genes%20in%20multiplex%20PCR%20for%20discriminating%20bovine%2C%20buffalo%20and%20porcine%20materials%20in%20food%20chain.pdf Hossain, M. A. Motalib and Ali, Md. Eaqub and Hamid, Sharifah Bee Abd and Asing and Mustafa, Shuhaimi and Desa, Mohd Nasir Mohd and Zaidul, I. S. M. (2017) Targeting double genes in multiplex PCR for discriminating bovine, buffalo and porcine materials in food chain. Food Control, 73 (pt. B). 175 - 184. ISSN 0956-7135; ESSN: 1873-7129 https://www.sciencedirect.com/science/article/pii/S0956713516304261 10.1016/j.foodcont.2016.08.008 |
| spellingShingle | Hossain, M. A. Motalib Ali, Md. Eaqub Hamid, Sharifah Bee Abd Asing Mustafa, Shuhaimi Desa, Mohd Nasir Mohd Zaidul, I. S. M. Targeting double genes in multiplex PCR for discriminating bovine, buffalo and porcine materials in food chain |
| title | Targeting double genes in multiplex PCR for discriminating bovine, buffalo and porcine materials in food chain |
| title_full | Targeting double genes in multiplex PCR for discriminating bovine, buffalo and porcine materials in food chain |
| title_fullStr | Targeting double genes in multiplex PCR for discriminating bovine, buffalo and porcine materials in food chain |
| title_full_unstemmed | Targeting double genes in multiplex PCR for discriminating bovine, buffalo and porcine materials in food chain |
| title_short | Targeting double genes in multiplex PCR for discriminating bovine, buffalo and porcine materials in food chain |
| title_sort | targeting double genes in multiplex pcr for discriminating bovine, buffalo and porcine materials in food chain |
| url | http://psasir.upm.edu.my/id/eprint/63382/ http://psasir.upm.edu.my/id/eprint/63382/ http://psasir.upm.edu.my/id/eprint/63382/ http://psasir.upm.edu.my/id/eprint/63382/1/Targeting%20double%20genes%20in%20multiplex%20PCR%20for%20discriminating%20bovine%2C%20buffalo%20and%20porcine%20materials%20in%20food%20chain.pdf |