Experimental review on the Substance P-enhanced endothelial permeability in human umbilical vein endothelial cells (HUVECS)
Inflammation is the immediate response to tissue damage or harmful stimuli. Though inconvenient, its role is significant and important as the protective and physiological response of our body. It directly sets the stage for tissue repair particularly increasing endothelial permeability which then co...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Malaysia Association of Research and Education for Educators
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61812/ http://psasir.upm.edu.my/id/eprint/61812/1/Experimental%20review%20on%20the%20Substance%20P-enhanced%20endothelial%20permeability%20in%20human%20umbilical%20vein%20endothelial%20cells%20%28HUVECS%29.pdf |
| _version_ | 1848854494153015296 |
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| author | N. N. H., Khairudin Z., Nurfarahdilla Ng, Chin Theng Fong, Lai Yen Perimal, Noch Kumar A., Zuraini Abdullah, Muhammad Nazrul Hakim |
| author_facet | N. N. H., Khairudin Z., Nurfarahdilla Ng, Chin Theng Fong, Lai Yen Perimal, Noch Kumar A., Zuraini Abdullah, Muhammad Nazrul Hakim |
| author_sort | N. N. H., Khairudin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Inflammation is the immediate response to tissue damage or harmful stimuli. Though inconvenient, its role is significant and important as the protective and physiological response of our body. It directly sets the stage for tissue repair particularly increasing endothelial permeability which then contributes to the healing process. However, in some cases inflammation may progress out of control causing various inflammatory diseases. Neurogenic inflammation is a sub-set of inflammation and is characterized by an increase in neuronal chemical mediators such as Substance P (SP). In this study, we investigated the involvement of SP in enhancing endothelial permeability on HUVECs monolayer. Neurogenic inflammation was induced through the administration of SP (1 nM to 100 nM) on HUVECs monolayer inserts, and incubated with varying short (10, 20 and 30 minutes) and longer (6, 12 and 24 hours) time-points. FITC-Dextran were finally added to cell culture inserts for 5 minutes to let the fluorescence molecule pass through the gaps. Endothelial permeability is directly proportional with extravasation of FITC-Dextran, determined by fluorescence intensity reading. Based on our data, there were no significant differences between control group (non-treated cell) and the different concentrations of SP at different time-points. Our current findings suggest that SP was unable to increase the endothelial permeability on HUVECs monolayer inflammatory experimental model. Experiments that use this model to mimic vascular inflammation in laboratory settings may require further elucidation in the future. |
| first_indexed | 2025-11-15T11:10:45Z |
| format | Article |
| id | upm-61812 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:10:45Z |
| publishDate | 2017 |
| publisher | Malaysia Association of Research and Education for Educators |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-618122019-01-29T06:40:12Z http://psasir.upm.edu.my/id/eprint/61812/ Experimental review on the Substance P-enhanced endothelial permeability in human umbilical vein endothelial cells (HUVECS) N. N. H., Khairudin Z., Nurfarahdilla Ng, Chin Theng Fong, Lai Yen Perimal, Noch Kumar A., Zuraini Abdullah, Muhammad Nazrul Hakim Inflammation is the immediate response to tissue damage or harmful stimuli. Though inconvenient, its role is significant and important as the protective and physiological response of our body. It directly sets the stage for tissue repair particularly increasing endothelial permeability which then contributes to the healing process. However, in some cases inflammation may progress out of control causing various inflammatory diseases. Neurogenic inflammation is a sub-set of inflammation and is characterized by an increase in neuronal chemical mediators such as Substance P (SP). In this study, we investigated the involvement of SP in enhancing endothelial permeability on HUVECs monolayer. Neurogenic inflammation was induced through the administration of SP (1 nM to 100 nM) on HUVECs monolayer inserts, and incubated with varying short (10, 20 and 30 minutes) and longer (6, 12 and 24 hours) time-points. FITC-Dextran were finally added to cell culture inserts for 5 minutes to let the fluorescence molecule pass through the gaps. Endothelial permeability is directly proportional with extravasation of FITC-Dextran, determined by fluorescence intensity reading. Based on our data, there were no significant differences between control group (non-treated cell) and the different concentrations of SP at different time-points. Our current findings suggest that SP was unable to increase the endothelial permeability on HUVECs monolayer inflammatory experimental model. Experiments that use this model to mimic vascular inflammation in laboratory settings may require further elucidation in the future. Malaysia Association of Research and Education for Educators 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61812/1/Experimental%20review%20on%20the%20Substance%20P-enhanced%20endothelial%20permeability%20in%20human%20umbilical%20vein%20endothelial%20cells%20%28HUVECS%29.pdf N. N. H., Khairudin and Z., Nurfarahdilla and Ng, Chin Theng and Fong, Lai Yen and Perimal, Noch Kumar and A., Zuraini and Abdullah, Muhammad Nazrul Hakim (2017) Experimental review on the Substance P-enhanced endothelial permeability in human umbilical vein endothelial cells (HUVECS). International Research Journal of Education and Sciences, 1 (2). 28 - 34. ISSN 2550-2158 http://www.masree.info/wp-content/uploads/2017/10/IRJES-VOL-1-ISSUE-2-ARTICLE-6.pdf |
| spellingShingle | N. N. H., Khairudin Z., Nurfarahdilla Ng, Chin Theng Fong, Lai Yen Perimal, Noch Kumar A., Zuraini Abdullah, Muhammad Nazrul Hakim Experimental review on the Substance P-enhanced endothelial permeability in human umbilical vein endothelial cells (HUVECS) |
| title | Experimental review on the Substance P-enhanced endothelial permeability in human umbilical vein endothelial cells (HUVECS) |
| title_full | Experimental review on the Substance P-enhanced endothelial permeability in human umbilical vein endothelial cells (HUVECS) |
| title_fullStr | Experimental review on the Substance P-enhanced endothelial permeability in human umbilical vein endothelial cells (HUVECS) |
| title_full_unstemmed | Experimental review on the Substance P-enhanced endothelial permeability in human umbilical vein endothelial cells (HUVECS) |
| title_short | Experimental review on the Substance P-enhanced endothelial permeability in human umbilical vein endothelial cells (HUVECS) |
| title_sort | experimental review on the substance p-enhanced endothelial permeability in human umbilical vein endothelial cells (huvecs) |
| url | http://psasir.upm.edu.my/id/eprint/61812/ http://psasir.upm.edu.my/id/eprint/61812/ http://psasir.upm.edu.my/id/eprint/61812/1/Experimental%20review%20on%20the%20Substance%20P-enhanced%20endothelial%20permeability%20in%20human%20umbilical%20vein%20endothelial%20cells%20%28HUVECS%29.pdf |