An update on Gardneralla vaginalis associated bacterial vaginosis in Malaysia
Objective: To update the status of Gardnerella vaginalis (G. vaginalis) as a causative agent of bacterial vaginosis (BV) in Malaysia and to define its epidemiology, metronidazole resistance and virulence properties. Methods: It is a single-centre (Gynaecology clinic at the Hospital Kuala Lumpur, Mal...
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| Format: | Article |
| Language: | English |
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Asian Pacific Journal of Tropical Biomedicine Editorial Office
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/60840/ http://psasir.upm.edu.my/id/eprint/60840/1/An%20update%20on%20Gardneralla%20vaginalis%20associated%20bacterial%20vaginosis%20in%20Malaysia.pdf |
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| author | Khairi Younus, Nada Gopinath, Renu Jegasothy, Ravindran Amin Nordin, Syafinaz Belkum, Alexvan Sither Joseph Vesudian, Narcisse Mary Neela, Vasantha Kumari |
| author_facet | Khairi Younus, Nada Gopinath, Renu Jegasothy, Ravindran Amin Nordin, Syafinaz Belkum, Alexvan Sither Joseph Vesudian, Narcisse Mary Neela, Vasantha Kumari |
| author_sort | Khairi Younus, Nada |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objective: To update the status of Gardnerella vaginalis (G. vaginalis) as a causative agent of bacterial vaginosis (BV) in Malaysia and to define its epidemiology, metronidazole resistance and virulence properties. Methods: It is a single-centre (Gynaecology clinic at the Hospital Kuala Lumpur, Malaysia) prospective study with laboratory-based microbiological follow up and analyses. Vaginal swabs collected from the patients suspected for BV were subjected to clinical BV diagnosis, isolation and identification of G. vaginalis, metronidazole susceptibility testing, vaginolysin and sialidase gene PCR, Piot's biotyping and amplified ribosomal DNA restriction analysis genotyping. Results: Among the 207 patients suspected for BV, G. vaginalis was isolated from 47 subjects. G. vaginalis coexisted with Trichomonas vaginalis and Candida albicans in 26 samples. Three G. vaginalis isolates were resistant to metronidazole. Biotyping revealed 1 and 7 as the common types. Amplified ribosomal DNA restriction analysis genotype II was found to be more common (n = 22; 46%) than I (n = 12; 25.53%) and III (n = 13; 27.6%). All genotype I and III isolates carried the sialidase gene, while 91.6% and 84.6% contained the vaginolysin gene. Genotype I was significantly associated with post-gynaecological surgical complications and abortions (P = 0.002). Conclusions: The existence of pathogenic G. vaginalis clones in Malaysia including drug resistant strains should not be taken lightly and needs to be monitored as these may bring more complications especially among women of child bearing age and pregnant women. |
| first_indexed | 2025-11-15T11:07:26Z |
| format | Article |
| id | upm-60840 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:07:26Z |
| publishDate | 2017 |
| publisher | Asian Pacific Journal of Tropical Biomedicine Editorial Office |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-608402019-04-26T08:59:24Z http://psasir.upm.edu.my/id/eprint/60840/ An update on Gardneralla vaginalis associated bacterial vaginosis in Malaysia Khairi Younus, Nada Gopinath, Renu Jegasothy, Ravindran Amin Nordin, Syafinaz Belkum, Alexvan Sither Joseph Vesudian, Narcisse Mary Neela, Vasantha Kumari Objective: To update the status of Gardnerella vaginalis (G. vaginalis) as a causative agent of bacterial vaginosis (BV) in Malaysia and to define its epidemiology, metronidazole resistance and virulence properties. Methods: It is a single-centre (Gynaecology clinic at the Hospital Kuala Lumpur, Malaysia) prospective study with laboratory-based microbiological follow up and analyses. Vaginal swabs collected from the patients suspected for BV were subjected to clinical BV diagnosis, isolation and identification of G. vaginalis, metronidazole susceptibility testing, vaginolysin and sialidase gene PCR, Piot's biotyping and amplified ribosomal DNA restriction analysis genotyping. Results: Among the 207 patients suspected for BV, G. vaginalis was isolated from 47 subjects. G. vaginalis coexisted with Trichomonas vaginalis and Candida albicans in 26 samples. Three G. vaginalis isolates were resistant to metronidazole. Biotyping revealed 1 and 7 as the common types. Amplified ribosomal DNA restriction analysis genotype II was found to be more common (n = 22; 46%) than I (n = 12; 25.53%) and III (n = 13; 27.6%). All genotype I and III isolates carried the sialidase gene, while 91.6% and 84.6% contained the vaginolysin gene. Genotype I was significantly associated with post-gynaecological surgical complications and abortions (P = 0.002). Conclusions: The existence of pathogenic G. vaginalis clones in Malaysia including drug resistant strains should not be taken lightly and needs to be monitored as these may bring more complications especially among women of child bearing age and pregnant women. Asian Pacific Journal of Tropical Biomedicine Editorial Office 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60840/1/An%20update%20on%20Gardneralla%20vaginalis%20associated%20bacterial%20vaginosis%20in%20Malaysia.pdf Khairi Younus, Nada and Gopinath, Renu and Jegasothy, Ravindran and Amin Nordin, Syafinaz and Belkum, Alexvan and Sither Joseph Vesudian, Narcisse Mary and Neela, Vasantha Kumari (2017) An update on Gardneralla vaginalis associated bacterial vaginosis in Malaysia. Asian Pacific Journal of Tropical Biomedicine, 7 (9). 831 - 835. ISSN 2221-1691 https://reader.elsevier.com/reader/sd/pii/S2221169117308559?token=F3F7B77D59327E99A2C49B8FD223F3FC0105EB8C8B39F1059224292F65ABEA12BA28352782B39DA7AB8D967C2403F7BD 10.1016/j.apjtb.2017.08.011 |
| spellingShingle | Khairi Younus, Nada Gopinath, Renu Jegasothy, Ravindran Amin Nordin, Syafinaz Belkum, Alexvan Sither Joseph Vesudian, Narcisse Mary Neela, Vasantha Kumari An update on Gardneralla vaginalis associated bacterial vaginosis in Malaysia |
| title | An update on Gardneralla vaginalis associated bacterial vaginosis in Malaysia |
| title_full | An update on Gardneralla vaginalis associated bacterial vaginosis in Malaysia |
| title_fullStr | An update on Gardneralla vaginalis associated bacterial vaginosis in Malaysia |
| title_full_unstemmed | An update on Gardneralla vaginalis associated bacterial vaginosis in Malaysia |
| title_short | An update on Gardneralla vaginalis associated bacterial vaginosis in Malaysia |
| title_sort | update on gardneralla vaginalis associated bacterial vaginosis in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/60840/ http://psasir.upm.edu.my/id/eprint/60840/ http://psasir.upm.edu.my/id/eprint/60840/ http://psasir.upm.edu.my/id/eprint/60840/1/An%20update%20on%20Gardneralla%20vaginalis%20associated%20bacterial%20vaginosis%20in%20Malaysia.pdf |