Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits
Biocellulose (BC) is pure extracellular cellulose produced by several species of micro-organisms that has numerous applications in the food, biomedical and paper industries. However, the existing biocellulose-producing bacterial strain with high yield was limited. The aim of this study was to isolat...
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| Format: | Article |
| Language: | English |
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The Society for Applied Microbiology
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/59683/ http://psasir.upm.edu.my/id/eprint/59683/1/Isolation%20and%20identification%20of%20biocellulose-producing%20bacterial%20strains%20from%20Malaysian%20acidic%20fruits.pdf |
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| author | Voon, Wendy Wen Yi Rukayadi, Yaya Meor Hussin, Anis Shobirin |
| author_facet | Voon, Wendy Wen Yi Rukayadi, Yaya Meor Hussin, Anis Shobirin |
| author_sort | Voon, Wendy Wen Yi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Biocellulose (BC) is pure extracellular cellulose produced by several species of micro-organisms that has numerous applications in the food, biomedical and paper industries. However, the existing biocellulose-producing bacterial strain with high yield was limited. The aim of this study was to isolate and identify the potential biocellulose-producing bacterial isolates from Malaysian acidic fruits. One hundred and ninety-three bacterial isolates were obtained from 19 local acidic fruits collected in Malaysia and screened for their ability to produce BC. A total of 15 potential bacterial isolates were then cultured in standard Hestrin-Schramm (HS) medium statically at 30°C for 2 weeks to determine the BC production. The most potent bacterial isolates were identified using 16S rRNA gene sequence analysis, morphological and biochemical characteristics. Three new and potent biocellulose-producing bacterial strains were isolated from soursop fruit and identified as Stenotrophomonas maltophilia WAUPM42, Pantoea vagans WAUPM45 and Beijerinckia fluminensis WAUPM53. Stenotrophomonas maltophilia WAUPM42 was the most potent biocellulose-producing bacterial strain that produced the highest amount of BC 0·58 g l−1 in standard HS medium. Whereas, the isolates P. vagans WAUPM45 and B. fluminensis WAUPM53 showed 0·50 and 0·52 g l−1 of BC production, respectively. |
| first_indexed | 2025-11-15T11:02:44Z |
| format | Article |
| id | upm-59683 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T11:02:44Z |
| publishDate | 2016 |
| publisher | The Society for Applied Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-596832018-03-16T02:45:19Z http://psasir.upm.edu.my/id/eprint/59683/ Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits Voon, Wendy Wen Yi Rukayadi, Yaya Meor Hussin, Anis Shobirin Biocellulose (BC) is pure extracellular cellulose produced by several species of micro-organisms that has numerous applications in the food, biomedical and paper industries. However, the existing biocellulose-producing bacterial strain with high yield was limited. The aim of this study was to isolate and identify the potential biocellulose-producing bacterial isolates from Malaysian acidic fruits. One hundred and ninety-three bacterial isolates were obtained from 19 local acidic fruits collected in Malaysia and screened for their ability to produce BC. A total of 15 potential bacterial isolates were then cultured in standard Hestrin-Schramm (HS) medium statically at 30°C for 2 weeks to determine the BC production. The most potent bacterial isolates were identified using 16S rRNA gene sequence analysis, morphological and biochemical characteristics. Three new and potent biocellulose-producing bacterial strains were isolated from soursop fruit and identified as Stenotrophomonas maltophilia WAUPM42, Pantoea vagans WAUPM45 and Beijerinckia fluminensis WAUPM53. Stenotrophomonas maltophilia WAUPM42 was the most potent biocellulose-producing bacterial strain that produced the highest amount of BC 0·58 g l−1 in standard HS medium. Whereas, the isolates P. vagans WAUPM45 and B. fluminensis WAUPM53 showed 0·50 and 0·52 g l−1 of BC production, respectively. The Society for Applied Microbiology 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/59683/1/Isolation%20and%20identification%20of%20biocellulose-producing%20bacterial%20strains%20from%20Malaysian%20acidic%20fruits.pdf Voon, Wendy Wen Yi and Rukayadi, Yaya and Meor Hussin, Anis Shobirin (2016) Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits. Letters in Applied Microbiology, 62 (5). pp. 428-433. ISSN 0266-8254; ESSN: 1472-765X http://onlinelibrary.wiley.com/wol1/doi/10.1111/lam.12568/abstract 10.1111/lam.12568 |
| spellingShingle | Voon, Wendy Wen Yi Rukayadi, Yaya Meor Hussin, Anis Shobirin Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits |
| title | Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits |
| title_full | Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits |
| title_fullStr | Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits |
| title_full_unstemmed | Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits |
| title_short | Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits |
| title_sort | isolation and identification of biocellulose-producing bacterial strains from malaysian acidic fruits |
| url | http://psasir.upm.edu.my/id/eprint/59683/ http://psasir.upm.edu.my/id/eprint/59683/ http://psasir.upm.edu.my/id/eprint/59683/ http://psasir.upm.edu.my/id/eprint/59683/1/Isolation%20and%20identification%20of%20biocellulose-producing%20bacterial%20strains%20from%20Malaysian%20acidic%20fruits.pdf |