Development of alginate – gum arabic beads for targeted delivery of protein

Controlled release beads were prepared by using the combination of alginate and gum Arabic through ionotropic gelation method. Bovine serum albumin was used as model protein for in vitro assessments. The effect of amount of sodium alginate and gum Arabic as the factor affecting protein encapsulation...

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Main Authors: Mohamed, Hajaratul Najwa, Mustafa, Shuhaimi, Fitrianto, Anwar, Abd Manap, Yazid
Format: Article
Language:English
Published: Omics Publishing Group 2017
Online Access:http://psasir.upm.edu.my/id/eprint/55489/
http://psasir.upm.edu.my/id/eprint/55489/1/Development%20of%20alginate%E2%80%93gum%20Arabic%20beads%20for%20targeted%20delivery%20of%20protein.pdf
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author Mohamed, Hajaratul Najwa
Mustafa, Shuhaimi
Fitrianto, Anwar
Abd Manap, Yazid
author_facet Mohamed, Hajaratul Najwa
Mustafa, Shuhaimi
Fitrianto, Anwar
Abd Manap, Yazid
author_sort Mohamed, Hajaratul Najwa
building UPM Institutional Repository
collection Online Access
description Controlled release beads were prepared by using the combination of alginate and gum Arabic through ionotropic gelation method. Bovine serum albumin was used as model protein for in vitro assessments. The effect of amount of sodium alginate and gum Arabic as the factor affecting protein encapsulation efficiency and protein release were optimized and analyzed by using RSM-FCCD. It was observed that protein encapsulation efficiency was increased and protein release was decreased with the increase of both of the amount of sodium alginate and gum Arabic, used as polymer blend. The optimized beads showed high encapsulation efficiency (87.5 ± 3.65%) with suitable protein release (100% protein release after almost 4 hrs). The swelling of beads were highly influenced by pH of dissolution medium. These beads were also characterized by FT-IR spectroscopy, SEM and TA for protein-excipients interaction, beads surface morphology and beads strength, respectively. These calcium alginate/gum Arabic beads have good potential to be used as delivery vehicle for protein drugs.
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institution Universiti Putra Malaysia
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language English
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publishDate 2017
publisher Omics Publishing Group
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spelling upm-554892017-10-05T03:19:41Z http://psasir.upm.edu.my/id/eprint/55489/ Development of alginate – gum arabic beads for targeted delivery of protein Mohamed, Hajaratul Najwa Mustafa, Shuhaimi Fitrianto, Anwar Abd Manap, Yazid Controlled release beads were prepared by using the combination of alginate and gum Arabic through ionotropic gelation method. Bovine serum albumin was used as model protein for in vitro assessments. The effect of amount of sodium alginate and gum Arabic as the factor affecting protein encapsulation efficiency and protein release were optimized and analyzed by using RSM-FCCD. It was observed that protein encapsulation efficiency was increased and protein release was decreased with the increase of both of the amount of sodium alginate and gum Arabic, used as polymer blend. The optimized beads showed high encapsulation efficiency (87.5 ± 3.65%) with suitable protein release (100% protein release after almost 4 hrs). The swelling of beads were highly influenced by pH of dissolution medium. These beads were also characterized by FT-IR spectroscopy, SEM and TA for protein-excipients interaction, beads surface morphology and beads strength, respectively. These calcium alginate/gum Arabic beads have good potential to be used as delivery vehicle for protein drugs. Omics Publishing Group 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55489/1/Development%20of%20alginate%E2%80%93gum%20Arabic%20beads%20for%20targeted%20delivery%20of%20protein.pdf Mohamed, Hajaratul Najwa and Mustafa, Shuhaimi and Fitrianto, Anwar and Abd Manap, Yazid (2017) Development of alginate – gum arabic beads for targeted delivery of protein. Journal of Biomolecular Research and Therapeutics, 3 (1). pp. 486-508. ISSN 2167-7956 http://www.omicsgroup.org/journals/jbmrthome.php 10.4172/2167-7956.1000155
spellingShingle Mohamed, Hajaratul Najwa
Mustafa, Shuhaimi
Fitrianto, Anwar
Abd Manap, Yazid
Development of alginate – gum arabic beads for targeted delivery of protein
title Development of alginate – gum arabic beads for targeted delivery of protein
title_full Development of alginate – gum arabic beads for targeted delivery of protein
title_fullStr Development of alginate – gum arabic beads for targeted delivery of protein
title_full_unstemmed Development of alginate – gum arabic beads for targeted delivery of protein
title_short Development of alginate – gum arabic beads for targeted delivery of protein
title_sort development of alginate – gum arabic beads for targeted delivery of protein
url http://psasir.upm.edu.my/id/eprint/55489/
http://psasir.upm.edu.my/id/eprint/55489/
http://psasir.upm.edu.my/id/eprint/55489/
http://psasir.upm.edu.my/id/eprint/55489/1/Development%20of%20alginate%E2%80%93gum%20Arabic%20beads%20for%20targeted%20delivery%20of%20protein.pdf