Detection of Citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor
Due to the low titer or uneven distribution of Citrus tristeza virus (CTV) in field samples, detection of CTV by using conventional detection techniques may be difficult. Therefore, in the present work, the cadmium-telluride quantum dots (QDs) was conjugated with a specific antibody against coat pro...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/55460/ http://psasir.upm.edu.my/id/eprint/55460/1/Detection%20of%20Citrus%20tristeza%20virus%20by%20using%20fluorescence%20resonance%20energy%20transfer-based%20biosensor.pdf |
| _version_ | 1848852808796733440 |
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| author | Shojaei, Taha Roodbar Mohd Salleh, Mohamad Amran Sijam, Kamaruzaman Abdul Rahim, Raha Mohsenifar, Afshin Safarnejad, Reza Tabatabaei, Meisam |
| author_facet | Shojaei, Taha Roodbar Mohd Salleh, Mohamad Amran Sijam, Kamaruzaman Abdul Rahim, Raha Mohsenifar, Afshin Safarnejad, Reza Tabatabaei, Meisam |
| author_sort | Shojaei, Taha Roodbar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Due to the low titer or uneven distribution of Citrus tristeza virus (CTV) in field samples, detection of CTV by using conventional detection techniques may be difficult. Therefore, in the present work, the cadmium-telluride quantum dots (QDs) was conjugated with a specific antibody against coat protein (CP) of CTV, and the CP were immobilized on the surface of gold nanoparticles (AuNPs) to develop a specific and sensitive fluorescence resonance energy transfer (FRET)-based nanobiosensor for detecting CTV. The maximum FRET efficiency for the developed nano-biosensor was observed at 60% in AuNPs-CP/QDs-Ab ratio of 1:8.5. The designed system showed higher sensitivity and specificity over enzyme linked immunosorbent assay (ELISA) with a limit of detection of 0.13μgmL(-1) and 93% and 94% sensitivity and specificity, respectively. As designed sensor is rapid, sensitive, specific and efficient in detecting CTV, this could be envisioned for diagnostic applications, surveillance and plant certification program. |
| first_indexed | 2025-11-15T10:43:58Z |
| format | Article |
| id | upm-55460 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:43:58Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-554602017-09-14T09:52:56Z http://psasir.upm.edu.my/id/eprint/55460/ Detection of Citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor Shojaei, Taha Roodbar Mohd Salleh, Mohamad Amran Sijam, Kamaruzaman Abdul Rahim, Raha Mohsenifar, Afshin Safarnejad, Reza Tabatabaei, Meisam Due to the low titer or uneven distribution of Citrus tristeza virus (CTV) in field samples, detection of CTV by using conventional detection techniques may be difficult. Therefore, in the present work, the cadmium-telluride quantum dots (QDs) was conjugated with a specific antibody against coat protein (CP) of CTV, and the CP were immobilized on the surface of gold nanoparticles (AuNPs) to develop a specific and sensitive fluorescence resonance energy transfer (FRET)-based nanobiosensor for detecting CTV. The maximum FRET efficiency for the developed nano-biosensor was observed at 60% in AuNPs-CP/QDs-Ab ratio of 1:8.5. The designed system showed higher sensitivity and specificity over enzyme linked immunosorbent assay (ELISA) with a limit of detection of 0.13μgmL(-1) and 93% and 94% sensitivity and specificity, respectively. As designed sensor is rapid, sensitive, specific and efficient in detecting CTV, this could be envisioned for diagnostic applications, surveillance and plant certification program. Elsevier 2016-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55460/1/Detection%20of%20Citrus%20tristeza%20virus%20by%20using%20fluorescence%20resonance%20energy%20transfer-based%20biosensor.pdf Shojaei, Taha Roodbar and Mohd Salleh, Mohamad Amran and Sijam, Kamaruzaman and Abdul Rahim, Raha and Mohsenifar, Afshin and Safarnejad, Reza and Tabatabaei, Meisam (2016) Detection of Citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 169. pp. 216-222. ISSN 1386-1425; ESSN: 1873-3557 10.1016/j.saa.2016.06.052 |
| spellingShingle | Shojaei, Taha Roodbar Mohd Salleh, Mohamad Amran Sijam, Kamaruzaman Abdul Rahim, Raha Mohsenifar, Afshin Safarnejad, Reza Tabatabaei, Meisam Detection of Citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor |
| title | Detection of Citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor |
| title_full | Detection of Citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor |
| title_fullStr | Detection of Citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor |
| title_full_unstemmed | Detection of Citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor |
| title_short | Detection of Citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor |
| title_sort | detection of citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor |
| url | http://psasir.upm.edu.my/id/eprint/55460/ http://psasir.upm.edu.my/id/eprint/55460/ http://psasir.upm.edu.my/id/eprint/55460/1/Detection%20of%20Citrus%20tristeza%20virus%20by%20using%20fluorescence%20resonance%20energy%20transfer-based%20biosensor.pdf |