Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak
Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molyb-denum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing b...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Faculty of Agriculture, University of Brawijaya, Indonesia
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54629/ http://psasir.upm.edu.my/id/eprint/54629/1/Isolation%20and%20characterisation%20of%20a%20molybdenum-reducing%20and%20glyphosate-degrading%20Klebsiella%20oxytoca%20strain%20SAW-5%20in%20solid%20from%20Sarawak%20.pdf |
| _version_ | 1848852596915175424 |
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| author | Sabullah, Mohd Khalizan Rahman, Mohd Fadhil Ahmad, Siti Aqlima Sulaiman, Mohd Rosni Shukor, Mohd Shukri Shamaan, Nor Aripin Abd Shukor, Mohd Yunus |
| author_facet | Sabullah, Mohd Khalizan Rahman, Mohd Fadhil Ahmad, Siti Aqlima Sulaiman, Mohd Rosni Shukor, Mohd Shukri Shamaan, Nor Aripin Abd Shukor, Mohd Yunus |
| author_sort | Sabullah, Mohd Khalizan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molyb-denum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34oC. Glucose was the best elec-tron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spec-trum resembling a reduced phospho-molybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants. |
| first_indexed | 2025-11-15T10:40:36Z |
| format | Article |
| id | upm-54629 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:40:36Z |
| publishDate | 2016 |
| publisher | Faculty of Agriculture, University of Brawijaya, Indonesia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-546292018-04-17T02:33:58Z http://psasir.upm.edu.my/id/eprint/54629/ Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak Sabullah, Mohd Khalizan Rahman, Mohd Fadhil Ahmad, Siti Aqlima Sulaiman, Mohd Rosni Shukor, Mohd Shukri Shamaan, Nor Aripin Abd Shukor, Mohd Yunus Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molyb-denum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34oC. Glucose was the best elec-tron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spec-trum resembling a reduced phospho-molybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants. Faculty of Agriculture, University of Brawijaya, Indonesia 2016-02 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54629/1/Isolation%20and%20characterisation%20of%20a%20molybdenum-reducing%20and%20glyphosate-degrading%20Klebsiella%20oxytoca%20strain%20SAW-5%20in%20solid%20from%20Sarawak%20.pdf Sabullah, Mohd Khalizan and Rahman, Mohd Fadhil and Ahmad, Siti Aqlima and Sulaiman, Mohd Rosni and Shukor, Mohd Shukri and Shamaan, Nor Aripin and Abd Shukor, Mohd Yunus (2016) Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak. Agrivita, 38 (1). pp. 1-13. ISSN 0126-0537; ESSN: 2477-8516 http://www.agrivita.ub.ac.id/index.php/agrivita/article/view/654 10.17503/agrivita.v38i1.654 |
| spellingShingle | Sabullah, Mohd Khalizan Rahman, Mohd Fadhil Ahmad, Siti Aqlima Sulaiman, Mohd Rosni Shukor, Mohd Shukri Shamaan, Nor Aripin Abd Shukor, Mohd Yunus Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak |
| title | Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak |
| title_full | Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak |
| title_fullStr | Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak |
| title_full_unstemmed | Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak |
| title_short | Isolation and characterisation of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain SAW-5 in solid from Sarawak |
| title_sort | isolation and characterisation of a molybdenum-reducing and glyphosate-degrading klebsiella oxytoca strain saw-5 in solid from sarawak |
| url | http://psasir.upm.edu.my/id/eprint/54629/ http://psasir.upm.edu.my/id/eprint/54629/ http://psasir.upm.edu.my/id/eprint/54629/ http://psasir.upm.edu.my/id/eprint/54629/1/Isolation%20and%20characterisation%20of%20a%20molybdenum-reducing%20and%20glyphosate-degrading%20Klebsiella%20oxytoca%20strain%20SAW-5%20in%20solid%20from%20Sarawak%20.pdf |