Identification and optimisation of lipase- catalysed synthesis of betulinic acid amide in a solvent system
Betulinic acid amide was synthesized from the enzymatic reaction of betulinic acid and butylamine catalysed by Novozym 435. The effects of different reaction parameters, such as effect of reaction time, reaction temperature, amount of enzyme, and substrate molar ratio (betulinic acid : butylamine),...
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| Language: | English |
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Hindawi Publishing Corporation
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/54479/ http://psasir.upm.edu.my/id/eprint/54479/1/Identification%20and%20optimisation%20of%20lipase-%20catalysed%20synthesis%20of%20betulinic%20acid%20amide%20in%20a%20solvent%20system.pdf |
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| author | Amin Yusof, Nurul Atikah Mat Hadzir, Nursyamsyila Ashari, Siti Efliza |
| author_facet | Amin Yusof, Nurul Atikah Mat Hadzir, Nursyamsyila Ashari, Siti Efliza |
| author_sort | Amin Yusof, Nurul Atikah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Betulinic acid amide was synthesized from the enzymatic reaction of betulinic acid and butylamine catalysed by Novozym 435. The effects of different reaction parameters, such as effect of reaction time, reaction temperature, amount of enzyme, and substrate molar ratio (betulinic acid : butylamine), were studied and conventionally optimised. Based on this study, the enzymatic synthesis of betulinic acid amide was found to be 64.6% at the optimum conditions of 24 h, 40°C, 100 mg enzyme, and 1 : 1 substrate molar ratio in 9 : 1 mixture of chloroform and hexane as solvent. The identification of final product was carried out using TLC, melting point, and FTIR and NMR showed the presence of betulinic acid amide. |
| first_indexed | 2025-11-15T10:39:56Z |
| format | Article |
| id | upm-54479 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:39:56Z |
| publishDate | 2016 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
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| spelling | upm-544792018-03-20T03:45:10Z http://psasir.upm.edu.my/id/eprint/54479/ Identification and optimisation of lipase- catalysed synthesis of betulinic acid amide in a solvent system Amin Yusof, Nurul Atikah Mat Hadzir, Nursyamsyila Ashari, Siti Efliza Betulinic acid amide was synthesized from the enzymatic reaction of betulinic acid and butylamine catalysed by Novozym 435. The effects of different reaction parameters, such as effect of reaction time, reaction temperature, amount of enzyme, and substrate molar ratio (betulinic acid : butylamine), were studied and conventionally optimised. Based on this study, the enzymatic synthesis of betulinic acid amide was found to be 64.6% at the optimum conditions of 24 h, 40°C, 100 mg enzyme, and 1 : 1 substrate molar ratio in 9 : 1 mixture of chloroform and hexane as solvent. The identification of final product was carried out using TLC, melting point, and FTIR and NMR showed the presence of betulinic acid amide. Hindawi Publishing Corporation 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54479/1/Identification%20and%20optimisation%20of%20lipase-%20catalysed%20synthesis%20of%20betulinic%20acid%20amide%20in%20a%20solvent%20system.pdf Amin Yusof, Nurul Atikah and Mat Hadzir, Nursyamsyila and Ashari, Siti Efliza (2016) Identification and optimisation of lipase- catalysed synthesis of betulinic acid amide in a solvent system. Journal of Applied Chemistry and Biotechnology, 2016. art. no. 5149326. pp. 1-5. ISSN 2356-7171; ESSN: 2314-6923 https://www.hindawi.com/journals/jac/2016/5149326/abs/ Lipase- catalysed synthesis; Betulinic acid amide; Solvent system 10.1155/2016/5149326 |
| spellingShingle | Lipase- catalysed synthesis; Betulinic acid amide; Solvent system Amin Yusof, Nurul Atikah Mat Hadzir, Nursyamsyila Ashari, Siti Efliza Identification and optimisation of lipase- catalysed synthesis of betulinic acid amide in a solvent system |
| title | Identification and optimisation of lipase- catalysed synthesis of betulinic acid amide in a solvent system |
| title_full | Identification and optimisation of lipase- catalysed synthesis of betulinic acid amide in a solvent system |
| title_fullStr | Identification and optimisation of lipase- catalysed synthesis of betulinic acid amide in a solvent system |
| title_full_unstemmed | Identification and optimisation of lipase- catalysed synthesis of betulinic acid amide in a solvent system |
| title_short | Identification and optimisation of lipase- catalysed synthesis of betulinic acid amide in a solvent system |
| title_sort | identification and optimisation of lipase- catalysed synthesis of betulinic acid amide in a solvent system |
| topic | Lipase- catalysed synthesis; Betulinic acid amide; Solvent system |
| url | http://psasir.upm.edu.my/id/eprint/54479/ http://psasir.upm.edu.my/id/eprint/54479/ http://psasir.upm.edu.my/id/eprint/54479/ http://psasir.upm.edu.my/id/eprint/54479/1/Identification%20and%20optimisation%20of%20lipase-%20catalysed%20synthesis%20of%20betulinic%20acid%20amide%20in%20a%20solvent%20system.pdf |