Multiple overlap extension PCR (MOE-PCR): an effective technical shortcut to high throughput synthetic biology

QR Code

Multiple overlap extension PCR (MOE-PCR): an effective technical shortcut to high throughput synthetic biology

The current study describes multiple-overlap-extension PCR (MOE-PCR) as a simple and effective approach to assembling multiple DNA fragments with various sizes and features in a single in vitro reaction. In this research, 50 bp of homology in overlapping DNA fragments and a specific touchdown PCR pr...

Full description

Bibliographic Details
Main Authors:	Kadkhodaei, Saeid, Memari, Hamid Rajabi, Abbasiliasi, Sahar, Rezaei, Morvarid Akhavan, Movahedi, Ali, Tan, Joo Shun, Ariff, Arbakariya
Format:	Article
Published:	Royal Society of Chemistry 2016
Online Access:	http://psasir.upm.edu.my/id/eprint/53351/

Similar Items

Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis
by: Hasnain, Hussain, et al.
Published: (2016)

Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension
by: Karim, Mohd Shahrul Nizwanshah, et al.
Published: (2017)

New expression vector for microalgae
by: Ariff, Arbakariya, et al.
Published: (2014)

No shortcuts to clean water
by: Zainudin, Zaki
Published: (2014)

Shortcuts and Balloons: The Aesthetics of Tropfest
by: McGrath, Kenta
Published: (2013)

Of mass media influence and advertisers' 'shortcuts'
by: Eastern Times Sarawak
Published: (2010)

An extension to artifact-free projection overlaps
by: Lin, Jianyu
Published: (2015)

DNA-Based Faecal Dietary Analysis: A Comparison of qPCR and High Throughput Sequencing Approaches
by: Murray, D., et al.
Published: (2011)

Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system
by: Hadi, Sofijan, et al.
Published: (2017)

Negotiating learning: are shortcuts good or bad for our students?
by: Mohd Hashim, Intan Hashimah, et al.
Published: (2007)

Negotiating Learning:Are shortcuts good or bad for our students?
by: Shuib, Munir
Published: (2007)

Enhancement of BLIS production by Pediococcus acidilactici kp10 in optimized fermentation conditions using an artificial neural network
by: Abbasiliasi, Sahar, et al.
Published: (2016)

High-throughput real-time PCR and melt curve analysis for sexing Southern Ocean seabirds using fecal samples
by: Faux, C., et al.
Published: (2014)

DNA profiling among egg and beef meat isolates of Escherichia coli by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and random amplified polymorphic DNA-PCR (RAPD-PCR)
by: Abd Mutalib, Sahilah, et al.
Published: (2010)

Kinetic modeling of bacteriocin-like inhibitory substance secretion by Pediococcus acidilactici Kp10 and its stability in food manufacturing conditions
by: Abbasiliasi, Sahar, et al.
Published: (2018)

Microtiter miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli
by: Tan, Joo Shun, et al.
Published: (2018)

Improved detection and monitoring of fungicide resistance in Blumeria graminis f. sp. hordei with high-throughput genotype quantification by digital PCR
by: Zulak, K., et al.
Published: (2018)

Application of PCR-RFLP technique and direct PCR-sequencing analysis to determine the relationships of Acanthamoeba
by: Manaf, Siti Ruhaya Abdul, et al.
Published: (2004)

Overlap PCR strategy and cloning of Prostate Specific Membrane Antigen (PSMA) gene in pichia pastoris yeast recombinant expression system / Nassrin Abduljalil Badroon
by: Abduljalil Badroon, Nassrin
Published: (2013)

MOE ASPIRES TOWARDS PRODUCING BALANCED AND HOLISTIC GRADUATES
by: MPRC, Pusat Media & Perhubungan Awam
Published: (2015)

qPCR analysis of quorum sensing genes of pseudomonas aeruginosa: Primer design and qPCR efficiency
by: Saiedatul Akmal, Mohamed Sedek, et al.
Published: (2023)

Important considerations in qRT-PCR
by: Phang, Ing Chia
Published: (2011)

Nested PCR analysis of Plasmodium parasites
by: Snounou, Georges, et al.
Published: (2002)

Application of PCR-ELISA in molecular diagnosis
by: Sue, Mei Jean, et al.
Published: (2014)

Development of mesoscopic imaging system for surface inspection / Moe Win
by: Moe , Win
Published: (2018)

MOE-intel elite internship serlah potensi mahasiswa UTM
by: Berita Harian

Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
by: Yusop, Zulkifli, et al.
Published: (2009)

Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
by: Zulkifli, Y., et al.
Published: (2009)

Design and development of multiplex real-time pcr (qPCR) for detection of streptococcus pneumoniae, klebsiella pneumoniae and haemophilus influenzae
by: Zahari, Nurul Izzaty Najwa
Published: (2024)

In situ PCR for detection and identification of fungal species
by: Bindslev, L., et al.
Published: (2002)

PCR-RFLP Profiling Of Several Cyprinid Fishes
by: Siti Mardhiah, Saharuddin
Published: (2011)

The development of real-time pcr for the detection of bacteraemia
by: Shieng, Gooi Choo
Published: (2016)

PCR-RDB assay for detection of vibrio cholerae
by: Yun, Foong Sui
Published: (2005)

Comparative molecular subtyping of Salmonella enterica serovar typhi by VNTR, rep-PCR and restricted AP-PCR / Sepideh Rezanejad Shirazi
by: Shirazi, Sepideh Rezanejad
Published: (2009)

Sensitivity of the nested-polymerase chain reaction (PCR) assay for Brugia malayi and signi®cance of `free' DNA in PCR-based assays
by: Cox-Singh, J., et al.
Published: (2000)

Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR
by: A. S., Fadzlina, et al.
Published: (2013)

Isolation and selection of reference genes for Ganoderma boninense gene expression study using quantitative real-time PCR (qPCR)
by: Lim, Fook Hwa, et al.
Published: (2014)

Comparison of real-time PCR, conventional PCR and RT-lamp for the detection of Coconut Cadang-Cadang Viroid variant in oil palm
by: Roslan, Nur Diyana, et al.
Published: (2023)

Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus
by: Yee, May Ye
Published: (2020)

Assessing MOE of LVL using non-destructive technique (vibration method)
by: Md. Tahir, Paridah, et al.
Published: (2013)