Multiple overlap extension PCR (MOE-PCR): an effective technical shortcut to high throughput synthetic biology

Multiple overlap extension PCR (MOE-PCR): an effective technical shortcut to high throughput synthetic biology

The current study describes multiple-overlap-extension PCR (MOE-PCR) as a simple and effective approach to assembling multiple DNA fragments with various sizes and features in a single in vitro reaction. In this research, 50 bp of homology in overlapping DNA fragments and a specific touchdown PCR pr...

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Bibliographic Details
Main Authors: Kadkhodaei, Saeid, Memari, Hamid Rajabi, Abbasiliasi, Sahar, Rezaei, Morvarid Akhavan, Movahedi, Ali, Tan, Joo Shun, Ariff, Arbakariya
Format: Article
Published: Royal Society of Chemistry 2016
Online Access:http://psasir.upm.edu.my/id/eprint/53351/

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http://psasir.upm.edu.my/id/eprint/53351/

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