Morphine antidependence of Erythroxylum cuneatum (Miq.) kKurz in neurotransmission processes in vitro
Opiate abuse has been studied to cause adaptive changes observed in the presynaptic release and the mediated-synaptic plasticity proteins. The involvement of neuronal SNARE proteins reveals the role of the neurotransmitter release in expressing the opioid actions. The present study was designed to d...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53335/ http://psasir.upm.edu.my/id/eprint/53335/1/Morphine%20Antidependence%20of%20Erythroxylum%20cuneatum.pdf |
| _version_ | 1848852255608930304 |
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| author | Suliman, Noor Azuin Mohd Moklas, Mohamad Aris Mat Taib, Che Norma Adenan, Mohd Ilham Baharuldin, Mohamad Taufik Hidayat Basir, Rusliza Amom, Zulkhairi |
| author_facet | Suliman, Noor Azuin Mohd Moklas, Mohamad Aris Mat Taib, Che Norma Adenan, Mohd Ilham Baharuldin, Mohamad Taufik Hidayat Basir, Rusliza Amom, Zulkhairi |
| author_sort | Suliman, Noor Azuin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Opiate abuse has been studied to cause adaptive changes observed in the presynaptic release and the mediated-synaptic plasticity proteins. The involvement of neuronal SNARE proteins reveals the role of the neurotransmitter release in expressing the opioid actions. The present study was designed to determine the effect of the alkaloid extract of Erythroxylum cuneatum (E. cuneatum) against chronic morphine and the influences of E. cuneatum on neurotransmission processes observed in vitro. The human neuroblastoma cell line, SK-N-SH, was treated with the morphine, methadone, or E. cuneatum. The cell lysates were collected and tested for α-synuclein, calmodulin, vesicle-associated membrane protein 2 (VAMP 2), and synaptotagmin 1. The extract of E. cuneatum was observed to upregulate the decreased expression of dependence proteins, namely, α-synuclein and calmodulin. The effects were comparable to methadone and control. The expressions of VAMP 2 and synaptotagmin 1 were normalised by the plant and methadone. The extract of E. cuneatum was postulated to treat dependence symptoms after chronic morphine and improve the soluble N-ethylmaleimide-sensitive factor activating protein receptor (SNARE) protein involved in synaptic vesicle after. |
| first_indexed | 2025-11-15T10:35:10Z |
| format | Article |
| id | upm-53335 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:35:10Z |
| publishDate | 2016 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-533352017-10-10T10:16:55Z http://psasir.upm.edu.my/id/eprint/53335/ Morphine antidependence of Erythroxylum cuneatum (Miq.) kKurz in neurotransmission processes in vitro Suliman, Noor Azuin Mohd Moklas, Mohamad Aris Mat Taib, Che Norma Adenan, Mohd Ilham Baharuldin, Mohamad Taufik Hidayat Basir, Rusliza Amom, Zulkhairi Opiate abuse has been studied to cause adaptive changes observed in the presynaptic release and the mediated-synaptic plasticity proteins. The involvement of neuronal SNARE proteins reveals the role of the neurotransmitter release in expressing the opioid actions. The present study was designed to determine the effect of the alkaloid extract of Erythroxylum cuneatum (E. cuneatum) against chronic morphine and the influences of E. cuneatum on neurotransmission processes observed in vitro. The human neuroblastoma cell line, SK-N-SH, was treated with the morphine, methadone, or E. cuneatum. The cell lysates were collected and tested for α-synuclein, calmodulin, vesicle-associated membrane protein 2 (VAMP 2), and synaptotagmin 1. The extract of E. cuneatum was observed to upregulate the decreased expression of dependence proteins, namely, α-synuclein and calmodulin. The effects were comparable to methadone and control. The expressions of VAMP 2 and synaptotagmin 1 were normalised by the plant and methadone. The extract of E. cuneatum was postulated to treat dependence symptoms after chronic morphine and improve the soluble N-ethylmaleimide-sensitive factor activating protein receptor (SNARE) protein involved in synaptic vesicle after. Hindawi Publishing Corporation 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53335/1/Morphine%20Antidependence%20of%20Erythroxylum%20cuneatum.pdf Suliman, Noor Azuin and Mohd Moklas, Mohamad Aris and Mat Taib, Che Norma and Adenan, Mohd Ilham and Baharuldin, Mohamad Taufik Hidayat and Basir, Rusliza and Amom, Zulkhairi (2016) Morphine antidependence of Erythroxylum cuneatum (Miq.) kKurz in neurotransmission processes in vitro. Evidence-Based Complementary and Alternative Medicine, 2016. art. no. 3517209. pp. 1-9. ISSN 1741-427X; ESSN: 1741-4288 https://www.hindawi.com/journals/ecam/2016/3517209/ 10.1155/2016/3517209 |
| spellingShingle | Suliman, Noor Azuin Mohd Moklas, Mohamad Aris Mat Taib, Che Norma Adenan, Mohd Ilham Baharuldin, Mohamad Taufik Hidayat Basir, Rusliza Amom, Zulkhairi Morphine antidependence of Erythroxylum cuneatum (Miq.) kKurz in neurotransmission processes in vitro |
| title | Morphine antidependence of Erythroxylum cuneatum (Miq.) kKurz in neurotransmission processes in vitro |
| title_full | Morphine antidependence of Erythroxylum cuneatum (Miq.) kKurz in neurotransmission processes in vitro |
| title_fullStr | Morphine antidependence of Erythroxylum cuneatum (Miq.) kKurz in neurotransmission processes in vitro |
| title_full_unstemmed | Morphine antidependence of Erythroxylum cuneatum (Miq.) kKurz in neurotransmission processes in vitro |
| title_short | Morphine antidependence of Erythroxylum cuneatum (Miq.) kKurz in neurotransmission processes in vitro |
| title_sort | morphine antidependence of erythroxylum cuneatum (miq.) kkurz in neurotransmission processes in vitro |
| url | http://psasir.upm.edu.my/id/eprint/53335/ http://psasir.upm.edu.my/id/eprint/53335/ http://psasir.upm.edu.my/id/eprint/53335/ http://psasir.upm.edu.my/id/eprint/53335/1/Morphine%20Antidependence%20of%20Erythroxylum%20cuneatum.pdf |