16S metagenomic microbial composition analysis of kefir grain using MEGAN and BaseSpace
Kefir is a unique cultured product traditionally made from the fermentation of milk with kefir grains. Metagenomics analysis of kefir grain is essential to understand the composition of microbial populations in the kefir grain. Many microbial populations have been reported in kefir grains from diffe...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52110/ http://psasir.upm.edu.my/id/eprint/52110/1/16S%20metagenomic%20microbial%20composition%20analysis%20of%20kefir%20grain%20using%20MEGAN%20and%20BaseSpace.pdf |
| _version_ | 1848852015881388032 |
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| author | Zamberi, Nur Rizi Mohamad, Nurul Elyani Yeap, Swee Keong Ky, Huynh Beh, Boon Kee Liew, Woan Charn Tan, Sheau Wei Ho, Wan Yong Boo, Sook Yee Chua, Yi Heng Mohammed Alitheen, Noorjahan Banu |
| author_facet | Zamberi, Nur Rizi Mohamad, Nurul Elyani Yeap, Swee Keong Ky, Huynh Beh, Boon Kee Liew, Woan Charn Tan, Sheau Wei Ho, Wan Yong Boo, Sook Yee Chua, Yi Heng Mohammed Alitheen, Noorjahan Banu |
| author_sort | Zamberi, Nur Rizi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Kefir is a unique cultured product traditionally made from the fermentation of milk with kefir grains. Metagenomics analysis of kefir grain is essential to understand the composition of microbial populations in the kefir grain. Many microbial populations have been reported in kefir grains from different parts of the world. Although the kefir from Malaysian kefir grain is regularly consumed locally, no report has been made on the kefir grain microbial profile. The present study used kefir grain obtained locally and the microbial composition in the kefir grain was determined using Next Generation Sequencing (NGS). The taxonomic results analysis obtained when using BaseSpace (Illumina) and MEGAN were compared. The software agreed that Lactobacillus genus dominated the samples and the predominant species was L. kefiranofaciens (81.45–91.93%) while L. kefiri (2.01–2.47%) was the second in abundance. The results suggested that Malaysian kefir grain contained the same top two predominant species using both software methods and the microbial composition between both software did not vary significantly. |
| first_indexed | 2025-11-15T10:31:22Z |
| format | Article |
| id | upm-52110 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:31:22Z |
| publishDate | 2016 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-521102017-05-05T02:58:17Z http://psasir.upm.edu.my/id/eprint/52110/ 16S metagenomic microbial composition analysis of kefir grain using MEGAN and BaseSpace Zamberi, Nur Rizi Mohamad, Nurul Elyani Yeap, Swee Keong Ky, Huynh Beh, Boon Kee Liew, Woan Charn Tan, Sheau Wei Ho, Wan Yong Boo, Sook Yee Chua, Yi Heng Mohammed Alitheen, Noorjahan Banu Kefir is a unique cultured product traditionally made from the fermentation of milk with kefir grains. Metagenomics analysis of kefir grain is essential to understand the composition of microbial populations in the kefir grain. Many microbial populations have been reported in kefir grains from different parts of the world. Although the kefir from Malaysian kefir grain is regularly consumed locally, no report has been made on the kefir grain microbial profile. The present study used kefir grain obtained locally and the microbial composition in the kefir grain was determined using Next Generation Sequencing (NGS). The taxonomic results analysis obtained when using BaseSpace (Illumina) and MEGAN were compared. The software agreed that Lactobacillus genus dominated the samples and the predominant species was L. kefiranofaciens (81.45–91.93%) while L. kefiri (2.01–2.47%) was the second in abundance. The results suggested that Malaysian kefir grain contained the same top two predominant species using both software methods and the microbial composition between both software did not vary significantly. Taylor & Francis 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52110/1/16S%20metagenomic%20microbial%20composition%20analysis%20of%20kefir%20grain%20using%20MEGAN%20and%20BaseSpace.pdf Zamberi, Nur Rizi and Mohamad, Nurul Elyani and Yeap, Swee Keong and Ky, Huynh and Beh, Boon Kee and Liew, Woan Charn and Tan, Sheau Wei and Ho, Wan Yong and Boo, Sook Yee and Chua, Yi Heng and Mohammed Alitheen, Noorjahan Banu (2016) 16S metagenomic microbial composition analysis of kefir grain using MEGAN and BaseSpace. Food Biotechnology, 30 (3). pp. 219-230. ISSN 0890-5436; ESSN: 1532-4249 http://www.tandfonline.com/doi/abs/10.1080/08905436.2016.1200987?journalCode=lfbt20 10.1080/08905436.2016.1200987 |
| spellingShingle | Zamberi, Nur Rizi Mohamad, Nurul Elyani Yeap, Swee Keong Ky, Huynh Beh, Boon Kee Liew, Woan Charn Tan, Sheau Wei Ho, Wan Yong Boo, Sook Yee Chua, Yi Heng Mohammed Alitheen, Noorjahan Banu 16S metagenomic microbial composition analysis of kefir grain using MEGAN and BaseSpace |
| title | 16S metagenomic microbial composition analysis of kefir grain using MEGAN and BaseSpace |
| title_full | 16S metagenomic microbial composition analysis of kefir grain using MEGAN and BaseSpace |
| title_fullStr | 16S metagenomic microbial composition analysis of kefir grain using MEGAN and BaseSpace |
| title_full_unstemmed | 16S metagenomic microbial composition analysis of kefir grain using MEGAN and BaseSpace |
| title_short | 16S metagenomic microbial composition analysis of kefir grain using MEGAN and BaseSpace |
| title_sort | 16s metagenomic microbial composition analysis of kefir grain using megan and basespace |
| url | http://psasir.upm.edu.my/id/eprint/52110/ http://psasir.upm.edu.my/id/eprint/52110/ http://psasir.upm.edu.my/id/eprint/52110/ http://psasir.upm.edu.my/id/eprint/52110/1/16S%20metagenomic%20microbial%20composition%20analysis%20of%20kefir%20grain%20using%20MEGAN%20and%20BaseSpace.pdf |