Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage
Bacteriophages are the viruses of bacteria and are widely distributed in the biosphere, exhibiting dramatic manifestations both in liquid cultures and on solid media. In this study, bacteriophages were isolated from different types of food (beef, chicken meats, cucumber, lettuce, clam, cockles and s...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/51687/ http://psasir.upm.edu.my/id/eprint/51687/1/%2859%29.pdf |
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| author | Thung, Tze Young Mohd Fadzil, Siti Norshafawatie Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari Chang, Wei San Loo, Yuet Ying Kuan, Chee Hao New, Chia Yeung Anyi, Ubong Bahumaish, Ramzi Othman Saeed Mahyudin, Nor Ainy Basri, Dayang Fredalina Wan Mohamed Radzi, Che Wan Jasimah Radu, Son |
| author_facet | Thung, Tze Young Mohd Fadzil, Siti Norshafawatie Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari Chang, Wei San Loo, Yuet Ying Kuan, Chee Hao New, Chia Yeung Anyi, Ubong Bahumaish, Ramzi Othman Saeed Mahyudin, Nor Ainy Basri, Dayang Fredalina Wan Mohamed Radzi, Che Wan Jasimah Radu, Son |
| author_sort | Thung, Tze Young |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Bacteriophages are the viruses of bacteria and are widely distributed in the biosphere, exhibiting dramatic manifestations both in liquid cultures and on solid media. In this study, bacteriophages were isolated from different types of food (beef, chicken meats, cucumber, lettuce, clam, cockles and shrimp) and sewage samples using 6 reference pathogen strains (Salmonella Enteritidis, Salmonella Typhimurium, Campylobacter jejuni, Vibrio parahaemolyticus, Listeria monocytogenes and Escherichia coli). A total of 29 bacteriophage isolates were obtained and further examined for titer via agar overlay assay. The titers were determined within the range of 108 to 1011 PFU/mL. Our results showed that diverse of bacteriophages are naturally present in a variety of foods. |
| first_indexed | 2025-11-15T10:29:30Z |
| format | Article |
| id | upm-51687 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:29:30Z |
| publishDate | 2017 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-516872017-04-27T09:55:06Z http://psasir.upm.edu.my/id/eprint/51687/ Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage Thung, Tze Young Mohd Fadzil, Siti Norshafawatie Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari Chang, Wei San Loo, Yuet Ying Kuan, Chee Hao New, Chia Yeung Anyi, Ubong Bahumaish, Ramzi Othman Saeed Mahyudin, Nor Ainy Basri, Dayang Fredalina Wan Mohamed Radzi, Che Wan Jasimah Radu, Son Bacteriophages are the viruses of bacteria and are widely distributed in the biosphere, exhibiting dramatic manifestations both in liquid cultures and on solid media. In this study, bacteriophages were isolated from different types of food (beef, chicken meats, cucumber, lettuce, clam, cockles and shrimp) and sewage samples using 6 reference pathogen strains (Salmonella Enteritidis, Salmonella Typhimurium, Campylobacter jejuni, Vibrio parahaemolyticus, Listeria monocytogenes and Escherichia coli). A total of 29 bacteriophage isolates were obtained and further examined for titer via agar overlay assay. The titers were determined within the range of 108 to 1011 PFU/mL. Our results showed that diverse of bacteriophages are naturally present in a variety of foods. Faculty of Food Science and Technology, Universiti Putra Malaysia 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51687/1/%2859%29.pdf Thung, Tze Young and Mohd Fadzil, Siti Norshafawatie and Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari and Chang, Wei San and Loo, Yuet Ying and Kuan, Chee Hao and New, Chia Yeung and Anyi, Ubong and Bahumaish, Ramzi Othman Saeed and Mahyudin, Nor Ainy and Basri, Dayang Fredalina and Wan Mohamed Radzi, Che Wan Jasimah and Radu, Son (2017) Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage. International Food Research Journal, 24 (1). pp. 450-454. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/24%20(01)%202017/(59).pdf |
| spellingShingle | Thung, Tze Young Mohd Fadzil, Siti Norshafawatie Premarathne, Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari Chang, Wei San Loo, Yuet Ying Kuan, Chee Hao New, Chia Yeung Anyi, Ubong Bahumaish, Ramzi Othman Saeed Mahyudin, Nor Ainy Basri, Dayang Fredalina Wan Mohamed Radzi, Che Wan Jasimah Radu, Son Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage |
| title | Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage |
| title_full | Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage |
| title_fullStr | Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage |
| title_full_unstemmed | Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage |
| title_short | Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage |
| title_sort | isolation of food-borne pathogen bacteriophages from retail food and environmental sewage |
| url | http://psasir.upm.edu.my/id/eprint/51687/ http://psasir.upm.edu.my/id/eprint/51687/ http://psasir.upm.edu.my/id/eprint/51687/1/%2859%29.pdf |