Production and characterisation of cellulase from solid state fermentation of rice straw by Trichoderma harzianum SNRS3
Research on production and the use of cellulase and xylanase by commercial microbial strains is widely reported. However, research on production of cellulase and xylanase by local isolates of Trichoderma harzianum known as potential cellulase producers is still very limited. T. harzianum SNRS3 was u...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/49692/ http://psasir.upm.edu.my/id/eprint/49692/1/06%20JTAS%200790-2015.pdf |
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| author | Rahnama, Nooshin Md. Shah, Umi Kalsom Foo, Hooi Ling Abdul Rahman, Nor Aini Ariff, Arbakariya |
| author_facet | Rahnama, Nooshin Md. Shah, Umi Kalsom Foo, Hooi Ling Abdul Rahman, Nor Aini Ariff, Arbakariya |
| author_sort | Rahnama, Nooshin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Research on production and the use of cellulase and xylanase by commercial microbial strains is widely reported. However, research on production of cellulase and xylanase by local isolates of Trichoderma harzianum known as potential cellulase producers is still very limited. T. harzianum SNRS3 was used for cellulase and xylanase production from rice straw under solid state fermentation. Our study revealed that unlike Trichoderma sp. that is normally associated with low amounts of β-glucosidase, insufficient to perform an efficient hydrolysis, T. harzianum SNRS3 could be considered as a potential β-glucosidase producer, but not an efficient xylanase producer. As a result of storage of the crude cellulase at room temperature, β-glucosidase activity only decreased to above 80% of its original activity at the end of the 3rd week of storage. The crude cellulase produced by T. harzianum SNRS3 could be industrially applied as the enzyme is still highly active at 60°C and over a wide range of acidic pH. |
| first_indexed | 2025-11-15T10:20:39Z |
| format | Article |
| id | upm-49692 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:20:39Z |
| publishDate | 2016 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-496922016-12-30T05:31:27Z http://psasir.upm.edu.my/id/eprint/49692/ Production and characterisation of cellulase from solid state fermentation of rice straw by Trichoderma harzianum SNRS3 Rahnama, Nooshin Md. Shah, Umi Kalsom Foo, Hooi Ling Abdul Rahman, Nor Aini Ariff, Arbakariya Research on production and the use of cellulase and xylanase by commercial microbial strains is widely reported. However, research on production of cellulase and xylanase by local isolates of Trichoderma harzianum known as potential cellulase producers is still very limited. T. harzianum SNRS3 was used for cellulase and xylanase production from rice straw under solid state fermentation. Our study revealed that unlike Trichoderma sp. that is normally associated with low amounts of β-glucosidase, insufficient to perform an efficient hydrolysis, T. harzianum SNRS3 could be considered as a potential β-glucosidase producer, but not an efficient xylanase producer. As a result of storage of the crude cellulase at room temperature, β-glucosidase activity only decreased to above 80% of its original activity at the end of the 3rd week of storage. The crude cellulase produced by T. harzianum SNRS3 could be industrially applied as the enzyme is still highly active at 60°C and over a wide range of acidic pH. Universiti Putra Malaysia Press 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/49692/1/06%20JTAS%200790-2015.pdf Rahnama, Nooshin and Md. Shah, Umi Kalsom and Foo, Hooi Ling and Abdul Rahman, Nor Aini and Ariff, Arbakariya (2016) Production and characterisation of cellulase from solid state fermentation of rice straw by Trichoderma harzianum SNRS3. Pertanika Journal of Tropical Agricultural Science, 39 (4). pp. 507-531. ISSN 1511-3701; ESSN: 2231-8542 http://pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2039%20(4)%20Nov.%202016/06%20JTAS%200790-2015.pdf |
| spellingShingle | Rahnama, Nooshin Md. Shah, Umi Kalsom Foo, Hooi Ling Abdul Rahman, Nor Aini Ariff, Arbakariya Production and characterisation of cellulase from solid state fermentation of rice straw by Trichoderma harzianum SNRS3 |
| title | Production and characterisation of cellulase from solid state fermentation of rice straw by Trichoderma harzianum SNRS3 |
| title_full | Production and characterisation of cellulase from solid state fermentation of rice straw by Trichoderma harzianum SNRS3 |
| title_fullStr | Production and characterisation of cellulase from solid state fermentation of rice straw by Trichoderma harzianum SNRS3 |
| title_full_unstemmed | Production and characterisation of cellulase from solid state fermentation of rice straw by Trichoderma harzianum SNRS3 |
| title_short | Production and characterisation of cellulase from solid state fermentation of rice straw by Trichoderma harzianum SNRS3 |
| title_sort | production and characterisation of cellulase from solid state fermentation of rice straw by trichoderma harzianum snrs3 |
| url | http://psasir.upm.edu.my/id/eprint/49692/ http://psasir.upm.edu.my/id/eprint/49692/ http://psasir.upm.edu.my/id/eprint/49692/1/06%20JTAS%200790-2015.pdf |