Surface display of glycosylated Tyrosinase related protein-2 (TRP-2) tumour antigen on Lactococcus lactis
Background:The exploitation of the surface display system of food and commensal lactic acid bacteria (LAB) for bacterial, viral, or protozoan antigen delivery has received strong interest recently. The Generally Regarded as Safe (GRAS) status of the Lactococcus lactis coupled with a non-recombinant...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BioMed Central
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46435/ http://psasir.upm.edu.my/id/eprint/46435/1/Surface%20display%20of%20glycosylated%20Tyrosinase%20related%20protein-2%20%28TRP-2%29%20tumour%20antigen%20on%20Lactococcus%20lactis.pdf |
| _version_ | 1848850659450814464 |
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| author | Kalyanasundram, Jeevanathan Suet, Lin Chia Ai, Adelene Lian Song Abdul Rahim, Raha A., Howard Young Yusoff, Khatijah |
| author_facet | Kalyanasundram, Jeevanathan Suet, Lin Chia Ai, Adelene Lian Song Abdul Rahim, Raha A., Howard Young Yusoff, Khatijah |
| author_sort | Kalyanasundram, Jeevanathan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background:The exploitation of the surface display system of food and commensal lactic acid bacteria (LAB) for bacterial, viral, or protozoan antigen delivery has received strong interest recently. The Generally Regarded as Safe (GRAS) status of the Lactococcus lactis coupled with a non-recombinant strategy of in-trans surface display, provide a safe platform for therapeutic drug and vaccine development. However, production of therapeutic proteins fused with cell-wall anchoring motifs is predominantly limited to prokaryotic expression systems. This presents a major disadvantage in the surface display system particularly when glycosylation has been recently identified to significantly enhance epitope presentation. In this study, the glycosylated murine Tyrosinase related protein-2 (TRP-2) with the ability to anchor onto the L. lactis cell wall was produced in suspension adapted Chinese Hamster Ovary (CHO-S) cells by expressing TRP-2 fused with cell wall anchoring LysM motif (cA) at the C-terminus.
Results: A total amount of 33 μg of partially purified TRP-2-cA from ~6.0 g in wet weight of CHO-S cells was purified by His-tag affinity chromatography. The purified TRP-2-cA protein was shown to be N-glycosylated and successfully anchored to the L. lactis cell wall.
Conclusions: Thus cell surface presentation of glycosylated mammalian antigens may now permit development of novel and inexpensive vaccine platforms. |
| first_indexed | 2025-11-15T10:09:48Z |
| format | Article |
| id | upm-46435 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:09:48Z |
| publishDate | 2015 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-464352018-03-27T04:13:25Z http://psasir.upm.edu.my/id/eprint/46435/ Surface display of glycosylated Tyrosinase related protein-2 (TRP-2) tumour antigen on Lactococcus lactis Kalyanasundram, Jeevanathan Suet, Lin Chia Ai, Adelene Lian Song Abdul Rahim, Raha A., Howard Young Yusoff, Khatijah Background:The exploitation of the surface display system of food and commensal lactic acid bacteria (LAB) for bacterial, viral, or protozoan antigen delivery has received strong interest recently. The Generally Regarded as Safe (GRAS) status of the Lactococcus lactis coupled with a non-recombinant strategy of in-trans surface display, provide a safe platform for therapeutic drug and vaccine development. However, production of therapeutic proteins fused with cell-wall anchoring motifs is predominantly limited to prokaryotic expression systems. This presents a major disadvantage in the surface display system particularly when glycosylation has been recently identified to significantly enhance epitope presentation. In this study, the glycosylated murine Tyrosinase related protein-2 (TRP-2) with the ability to anchor onto the L. lactis cell wall was produced in suspension adapted Chinese Hamster Ovary (CHO-S) cells by expressing TRP-2 fused with cell wall anchoring LysM motif (cA) at the C-terminus. Results: A total amount of 33 μg of partially purified TRP-2-cA from ~6.0 g in wet weight of CHO-S cells was purified by His-tag affinity chromatography. The purified TRP-2-cA protein was shown to be N-glycosylated and successfully anchored to the L. lactis cell wall. Conclusions: Thus cell surface presentation of glycosylated mammalian antigens may now permit development of novel and inexpensive vaccine platforms. BioMed Central 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46435/1/Surface%20display%20of%20glycosylated%20Tyrosinase%20related%20protein-2%20%28TRP-2%29%20tumour%20antigen%20on%20Lactococcus%20lactis.pdf Kalyanasundram, Jeevanathan and Suet, Lin Chia and Ai, Adelene Lian Song and Abdul Rahim, Raha and A., Howard Young and Yusoff, Khatijah (2015) Surface display of glycosylated Tyrosinase related protein-2 (TRP-2) tumour antigen on Lactococcus lactis. BMC Biotechnology, 15 (113). p. 113. ISSN ESSN: 1472-6750 10.1186/s12896-015-0231-z |
| spellingShingle | Kalyanasundram, Jeevanathan Suet, Lin Chia Ai, Adelene Lian Song Abdul Rahim, Raha A., Howard Young Yusoff, Khatijah Surface display of glycosylated Tyrosinase related protein-2 (TRP-2) tumour antigen on Lactococcus lactis |
| title | Surface display of glycosylated Tyrosinase related protein-2 (TRP-2) tumour antigen on Lactococcus lactis |
| title_full | Surface display of glycosylated Tyrosinase related protein-2 (TRP-2) tumour antigen on Lactococcus lactis |
| title_fullStr | Surface display of glycosylated Tyrosinase related protein-2 (TRP-2) tumour antigen on Lactococcus lactis |
| title_full_unstemmed | Surface display of glycosylated Tyrosinase related protein-2 (TRP-2) tumour antigen on Lactococcus lactis |
| title_short | Surface display of glycosylated Tyrosinase related protein-2 (TRP-2) tumour antigen on Lactococcus lactis |
| title_sort | surface display of glycosylated tyrosinase related protein-2 (trp-2) tumour antigen on lactococcus lactis |
| url | http://psasir.upm.edu.my/id/eprint/46435/ http://psasir.upm.edu.my/id/eprint/46435/ http://psasir.upm.edu.my/id/eprint/46435/1/Surface%20display%20of%20glycosylated%20Tyrosinase%20related%20protein-2%20%28TRP-2%29%20tumour%20antigen%20on%20Lactococcus%20lactis.pdf |