Rapid growth and osteogenic differentiation of mesenchymal stem cells isolated from human bone marrow
Mesenchymal stem cells (MSCs) are involved in bone formation in the embryo, bone repair and remodeling. The differentiation of these cells is a complex multistep pathway that involves discrete cellular transitions and is similar to that which occurs during hematopoiesis. MSCs have self-renewal capac...
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| Format: | Article |
| Language: | English |
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Spandidos Publications
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46080/ http://psasir.upm.edu.my/id/eprint/46080/1/Rapid%20growth%20and%20osteogenic%20differentiation%20of%20mesenchymal%20stem%20cells%20isolated%20from%20human%20bone%20marrow.pdf |
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| author | Shima, Wan nazatul Ali, Abdul Manaf Subramani, Tamilselvan Mohamed Alitheen, Noorjahan Banu Hamid, Muhajir Samsudin, Abdul Rani Yeap, Swee Keong |
| author_facet | Shima, Wan nazatul Ali, Abdul Manaf Subramani, Tamilselvan Mohamed Alitheen, Noorjahan Banu Hamid, Muhajir Samsudin, Abdul Rani Yeap, Swee Keong |
| author_sort | Shima, Wan nazatul |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Mesenchymal stem cells (MSCs) are involved in bone formation in the embryo, bone repair and remodeling. The differentiation of these cells is a complex multistep pathway that involves discrete cellular transitions and is similar to that which occurs during hematopoiesis. MSCs have self-renewal capacity without differentiation in long-term culture. In the present study, MSCs were isolated from human bone marrow and characterized by the presence of cluster of differentiation 105 marker using the labeled streptavidin biotin method. The MSCs were cultured in Dulbecco's modified Eagle's medium supplemented with fetal bovine serum, ascorbic acid, β-glycerol phosphate and dexamethasone to differentiate into osteoblasts. Biological in vitro analysis showed the rapid proliferation of the MSCs. Further evaluation of specific osteogenic markers using von Kossa staining and the alkaline phosphate assay demonstrated that the MSCs were stimulated to differentiate into osteoblast-lineage cells. This mesengenic potential indicated that the bone marrow-derived cells were multipotent MSCs. The findings of this study show that bone marrow can be a legitimate source of MSCs for the production of osteoblasts for utilization in bone replacement therapy. |
| first_indexed | 2025-11-15T10:08:43Z |
| format | Article |
| id | upm-46080 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T10:08:43Z |
| publishDate | 2015 |
| publisher | Spandidos Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-460802022-03-23T06:23:07Z http://psasir.upm.edu.my/id/eprint/46080/ Rapid growth and osteogenic differentiation of mesenchymal stem cells isolated from human bone marrow Shima, Wan nazatul Ali, Abdul Manaf Subramani, Tamilselvan Mohamed Alitheen, Noorjahan Banu Hamid, Muhajir Samsudin, Abdul Rani Yeap, Swee Keong Mesenchymal stem cells (MSCs) are involved in bone formation in the embryo, bone repair and remodeling. The differentiation of these cells is a complex multistep pathway that involves discrete cellular transitions and is similar to that which occurs during hematopoiesis. MSCs have self-renewal capacity without differentiation in long-term culture. In the present study, MSCs were isolated from human bone marrow and characterized by the presence of cluster of differentiation 105 marker using the labeled streptavidin biotin method. The MSCs were cultured in Dulbecco's modified Eagle's medium supplemented with fetal bovine serum, ascorbic acid, β-glycerol phosphate and dexamethasone to differentiate into osteoblasts. Biological in vitro analysis showed the rapid proliferation of the MSCs. Further evaluation of specific osteogenic markers using von Kossa staining and the alkaline phosphate assay demonstrated that the MSCs were stimulated to differentiate into osteoblast-lineage cells. This mesengenic potential indicated that the bone marrow-derived cells were multipotent MSCs. The findings of this study show that bone marrow can be a legitimate source of MSCs for the production of osteoblasts for utilization in bone replacement therapy. Spandidos Publications 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46080/1/Rapid%20growth%20and%20osteogenic%20differentiation%20of%20mesenchymal%20stem%20cells%20isolated%20from%20human%20bone%20marrow.pdf Shima, Wan nazatul and Ali, Abdul Manaf and Subramani, Tamilselvan and Mohamed Alitheen, Noorjahan Banu and Hamid, Muhajir and Samsudin, Abdul Rani and Yeap, Swee Keong (2015) Rapid growth and osteogenic differentiation of mesenchymal stem cells isolated from human bone marrow. Experimental and Therapeutic Medicine, 9 (6). pp. 2202-2206. ISSN 1792-0981; ESSN: 1792-1015 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4473366/ 10.3892/etm.2015.2381 |
| spellingShingle | Shima, Wan nazatul Ali, Abdul Manaf Subramani, Tamilselvan Mohamed Alitheen, Noorjahan Banu Hamid, Muhajir Samsudin, Abdul Rani Yeap, Swee Keong Rapid growth and osteogenic differentiation of mesenchymal stem cells isolated from human bone marrow |
| title | Rapid growth and osteogenic differentiation of mesenchymal stem cells isolated from human bone marrow |
| title_full | Rapid growth and osteogenic differentiation of mesenchymal stem cells isolated from human bone marrow |
| title_fullStr | Rapid growth and osteogenic differentiation of mesenchymal stem cells isolated from human bone marrow |
| title_full_unstemmed | Rapid growth and osteogenic differentiation of mesenchymal stem cells isolated from human bone marrow |
| title_short | Rapid growth and osteogenic differentiation of mesenchymal stem cells isolated from human bone marrow |
| title_sort | rapid growth and osteogenic differentiation of mesenchymal stem cells isolated from human bone marrow |
| url | http://psasir.upm.edu.my/id/eprint/46080/ http://psasir.upm.edu.my/id/eprint/46080/ http://psasir.upm.edu.my/id/eprint/46080/ http://psasir.upm.edu.my/id/eprint/46080/1/Rapid%20growth%20and%20osteogenic%20differentiation%20of%20mesenchymal%20stem%20cells%20isolated%20from%20human%20bone%20marrow.pdf |