Cockle shell-derived aragonite CaCO3 nanoparticles for co-delivery of doxorubicin and thymoquinone eliminates cancer stem cells
Cancer stem cells CSCs (tumour-initiating cells) are responsible for cancer metastasis and recurrence associated with resistance to conventional chemotherapy. This study generated MBA MD231 3D cancer stem cells enriched spheroids in serum-free conditions and evaluated the influence of combined doxor...
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| Format: | Article |
| Language: | English |
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MDPI
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38125/ http://psasir.upm.edu.my/id/eprint/38125/1/38125.pdf |
| _version_ | 1848848791889772544 |
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| author | Ibiyeye, Kehinde Muibat Abu Bakar @ Zakaria, Md Zuki |
| author_facet | Ibiyeye, Kehinde Muibat Abu Bakar @ Zakaria, Md Zuki |
| author_sort | Ibiyeye, Kehinde Muibat |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Cancer stem cells CSCs (tumour-initiating cells) are responsible for cancer metastasis and recurrence associated with resistance to conventional chemotherapy. This study generated MBA MD231 3D cancer stem cells enriched spheroids in serum-free conditions and evaluated the influence of combined doxorubicin/thymoquinone-loaded cockle-shell-derived aragonite calcium carbonate nanoparticles. Single loaded drugs and free drugs were also evaluated. WST assay, sphere forming assay, ALDH activity analysis, Surface marker of CD44 and CD24 expression, apoptosis with Annexin V-PI kit, cell cycle analysis, morphological changes using a phase contrast light microscope, scanning electron microscopy, invasion assay and migration assay were carried out; The combination therapy showed enhanced apoptosis, reduction in ALDH activity and expression of CD44 and CD24 surface maker, reduction in cellular migration and invasion, inhibition of 3D sphere formation when compared to the free drugs and the single drug-loaded nanoparticle. Scanning electron microscopy showed poor spheroid formation, cell membrane blebbing, presence of cell shrinkage, distortion in the spheroid architecture; and the results from this study showed that combined drug-loaded cockle-shell-derived aragonite calcium carbonate nanoparticles can efficiently destroy the breast CSCs compared to single drug-loaded nanoparticle and a simple mixture of doxorubicin and thymoquinone. |
| first_indexed | 2025-11-15T09:40:07Z |
| format | Article |
| id | upm-38125 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:40:07Z |
| publishDate | 2020 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-381252020-05-03T22:53:00Z http://psasir.upm.edu.my/id/eprint/38125/ Cockle shell-derived aragonite CaCO3 nanoparticles for co-delivery of doxorubicin and thymoquinone eliminates cancer stem cells Ibiyeye, Kehinde Muibat Abu Bakar @ Zakaria, Md Zuki Cancer stem cells CSCs (tumour-initiating cells) are responsible for cancer metastasis and recurrence associated with resistance to conventional chemotherapy. This study generated MBA MD231 3D cancer stem cells enriched spheroids in serum-free conditions and evaluated the influence of combined doxorubicin/thymoquinone-loaded cockle-shell-derived aragonite calcium carbonate nanoparticles. Single loaded drugs and free drugs were also evaluated. WST assay, sphere forming assay, ALDH activity analysis, Surface marker of CD44 and CD24 expression, apoptosis with Annexin V-PI kit, cell cycle analysis, morphological changes using a phase contrast light microscope, scanning electron microscopy, invasion assay and migration assay were carried out; The combination therapy showed enhanced apoptosis, reduction in ALDH activity and expression of CD44 and CD24 surface maker, reduction in cellular migration and invasion, inhibition of 3D sphere formation when compared to the free drugs and the single drug-loaded nanoparticle. Scanning electron microscopy showed poor spheroid formation, cell membrane blebbing, presence of cell shrinkage, distortion in the spheroid architecture; and the results from this study showed that combined drug-loaded cockle-shell-derived aragonite calcium carbonate nanoparticles can efficiently destroy the breast CSCs compared to single drug-loaded nanoparticle and a simple mixture of doxorubicin and thymoquinone. MDPI 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38125/1/38125.pdf Ibiyeye, Kehinde Muibat and Abu Bakar @ Zakaria, Md Zuki (2020) Cockle shell-derived aragonite CaCO3 nanoparticles for co-delivery of doxorubicin and thymoquinone eliminates cancer stem cells. International Journal of Molecular Sciences, 21 (5). art. no. 1900. pp. 1-29. ISSN 1661-6596; ESSN: 1422-0067 https://www.mdpi.com/1422-0067/21/5/1900 10.3390/ijms21051900 |
| spellingShingle | Ibiyeye, Kehinde Muibat Abu Bakar @ Zakaria, Md Zuki Cockle shell-derived aragonite CaCO3 nanoparticles for co-delivery of doxorubicin and thymoquinone eliminates cancer stem cells |
| title | Cockle shell-derived aragonite CaCO3 nanoparticles for co-delivery of doxorubicin and thymoquinone eliminates cancer stem cells |
| title_full | Cockle shell-derived aragonite CaCO3 nanoparticles for co-delivery of doxorubicin and thymoquinone eliminates cancer stem cells |
| title_fullStr | Cockle shell-derived aragonite CaCO3 nanoparticles for co-delivery of doxorubicin and thymoquinone eliminates cancer stem cells |
| title_full_unstemmed | Cockle shell-derived aragonite CaCO3 nanoparticles for co-delivery of doxorubicin and thymoquinone eliminates cancer stem cells |
| title_short | Cockle shell-derived aragonite CaCO3 nanoparticles for co-delivery of doxorubicin and thymoquinone eliminates cancer stem cells |
| title_sort | cockle shell-derived aragonite caco3 nanoparticles for co-delivery of doxorubicin and thymoquinone eliminates cancer stem cells |
| url | http://psasir.upm.edu.my/id/eprint/38125/ http://psasir.upm.edu.my/id/eprint/38125/ http://psasir.upm.edu.my/id/eprint/38125/ http://psasir.upm.edu.my/id/eprint/38125/1/38125.pdf |