Photoreceptor therapy: generation of neurosphere-like cells from human mesenchymal stem cells expressing erythropoietin
The loss of photoreceptors is a major concern implicated in age-macular degeneration (AMD), a type of neurodegenerative disorder. Failure to prescribe a suitable treatment due to the lack of understanding of the molecular pathogenesis, and limited capacity to compensate irreparably damaged photorece...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38077/ http://psasir.upm.edu.my/id/eprint/38077/1/Photoreceptor%20therapy%20generation%20of%20neurosphere-like%20cells%20from%20human%20mesenchymal%20stem%20cells%20expressing%20erythropoietin.pdf |
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| author | Mok, Pooi Ling Ding, Shirley Suet Lee Farhana, Aisha Alzahrani, Badr Khan, Mohammed Safwan Ali Subbiah, Suresh Kumar |
| author_facet | Mok, Pooi Ling Ding, Shirley Suet Lee Farhana, Aisha Alzahrani, Badr Khan, Mohammed Safwan Ali Subbiah, Suresh Kumar |
| author_sort | Mok, Pooi Ling |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The loss of photoreceptors is a major concern implicated in age-macular degeneration (AMD), a type of neurodegenerative disorder. Failure to prescribe a suitable treatment due to the lack of understanding of the molecular pathogenesis, and limited capacity to compensate irreparably damaged photoreceptors in the retina have greatly contributed to the progression of visual dysfunction. Our previous study has shown that Mesenchymal Stem Cells (MSCs) expressing erythropoietin (EPO) could commit into photoreceptor cell lineage. However, the efficiency of cell differentiation is limited. The present study aims to explore the capacity of these MSCs to form neurospheres. The cells were transduced with lentiviral particles encoding for human EPO and green fluorescent protein (GFP) genes, culture-expanded and sorted before subjected for differentiation induction into neural precursor cells. Our results showed that MSC-EPO developed into larger neurosphere and expressed relatively higher expression of nestin compared with MSCs alone when cultured under neural induction medium. These preliminary findings suggested that MSC-EPO have greater neurogenic potential than MSCs alone. Further study is needed to evaluate the possibilities of neurosphere to differentiate into functional photoreceptor cells. We believe that the success of neurosphere expansion may potentially be useful in scaling up the manufacturing of photoreceptors in a shorter time and at an efficient cost for retinal cell replacement therapy. |
| first_indexed | 2025-11-15T09:39:54Z |
| format | Article |
| id | upm-38077 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:39:54Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-380772020-04-14T14:04:37Z http://psasir.upm.edu.my/id/eprint/38077/ Photoreceptor therapy: generation of neurosphere-like cells from human mesenchymal stem cells expressing erythropoietin Mok, Pooi Ling Ding, Shirley Suet Lee Farhana, Aisha Alzahrani, Badr Khan, Mohammed Safwan Ali Subbiah, Suresh Kumar The loss of photoreceptors is a major concern implicated in age-macular degeneration (AMD), a type of neurodegenerative disorder. Failure to prescribe a suitable treatment due to the lack of understanding of the molecular pathogenesis, and limited capacity to compensate irreparably damaged photoreceptors in the retina have greatly contributed to the progression of visual dysfunction. Our previous study has shown that Mesenchymal Stem Cells (MSCs) expressing erythropoietin (EPO) could commit into photoreceptor cell lineage. However, the efficiency of cell differentiation is limited. The present study aims to explore the capacity of these MSCs to form neurospheres. The cells were transduced with lentiviral particles encoding for human EPO and green fluorescent protein (GFP) genes, culture-expanded and sorted before subjected for differentiation induction into neural precursor cells. Our results showed that MSC-EPO developed into larger neurosphere and expressed relatively higher expression of nestin compared with MSCs alone when cultured under neural induction medium. These preliminary findings suggested that MSC-EPO have greater neurogenic potential than MSCs alone. Further study is needed to evaluate the possibilities of neurosphere to differentiate into functional photoreceptor cells. We believe that the success of neurosphere expansion may potentially be useful in scaling up the manufacturing of photoreceptors in a shorter time and at an efficient cost for retinal cell replacement therapy. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38077/1/Photoreceptor%20therapy%20generation%20of%20neurosphere-like%20cells%20from%20human%20mesenchymal%20stem%20cells%20expressing%20erythropoietin.pdf Mok, Pooi Ling and Ding, Shirley Suet Lee and Farhana, Aisha and Alzahrani, Badr and Khan, Mohammed Safwan Ali and Subbiah, Suresh Kumar (2020) Photoreceptor therapy: generation of neurosphere-like cells from human mesenchymal stem cells expressing erythropoietin. Sains Malaysiana, 49 (1). pp. 113-119. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol49num1_2020/contentsVol49num1_2020.html 10.17576/jsm-2020-4901-13 |
| spellingShingle | Mok, Pooi Ling Ding, Shirley Suet Lee Farhana, Aisha Alzahrani, Badr Khan, Mohammed Safwan Ali Subbiah, Suresh Kumar Photoreceptor therapy: generation of neurosphere-like cells from human mesenchymal stem cells expressing erythropoietin |
| title | Photoreceptor therapy: generation of neurosphere-like cells from human mesenchymal stem cells expressing erythropoietin |
| title_full | Photoreceptor therapy: generation of neurosphere-like cells from human mesenchymal stem cells expressing erythropoietin |
| title_fullStr | Photoreceptor therapy: generation of neurosphere-like cells from human mesenchymal stem cells expressing erythropoietin |
| title_full_unstemmed | Photoreceptor therapy: generation of neurosphere-like cells from human mesenchymal stem cells expressing erythropoietin |
| title_short | Photoreceptor therapy: generation of neurosphere-like cells from human mesenchymal stem cells expressing erythropoietin |
| title_sort | photoreceptor therapy: generation of neurosphere-like cells from human mesenchymal stem cells expressing erythropoietin |
| url | http://psasir.upm.edu.my/id/eprint/38077/ http://psasir.upm.edu.my/id/eprint/38077/ http://psasir.upm.edu.my/id/eprint/38077/ http://psasir.upm.edu.my/id/eprint/38077/1/Photoreceptor%20therapy%20generation%20of%20neurosphere-like%20cells%20from%20human%20mesenchymal%20stem%20cells%20expressing%20erythropoietin.pdf |