In vitro modulation of probiotic bacteria on the biofilm of Candida glabrata
A conspicuous new concept of pathogens living as the microbial societies in the human host rather than free planktonic cells has raised considerable concerns among scientists and clinicians. Fungal biofilms are communities of cells that possess distinct characteristic such as increased resistance to...
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| Format: | Article |
| Language: | English |
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Elsevier
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/38010/ http://psasir.upm.edu.my/id/eprint/38010/1/In%20vitro%20modulation%20of%20probiotic%20bacteria%20on%20the%20biofilm%20of%20Candida%20glabrata.pdf |
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| author | Chew, Shu Yih Cheah, Yoke Kqueen Seow, Heng Fong Sandai, Doblin Than, Leslie Thian Lung |
| author_facet | Chew, Shu Yih Cheah, Yoke Kqueen Seow, Heng Fong Sandai, Doblin Than, Leslie Thian Lung |
| author_sort | Chew, Shu Yih |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A conspicuous new concept of pathogens living as the microbial societies in the human host rather than free planktonic cells has raised considerable concerns among scientists and clinicians. Fungal biofilms are communities of cells that possess distinct characteristic such as increased resistance to the immune defence and antimycotic agents in comparison to their planktonic cells counterpart. Therefore, inhibition of the biofilm may represent a new paradigm for antifungal development. In this study, we aim to evaluate the in vitro modulation of vulvovaginal candidiasis (VVC)-causing Candida glabrata biofilms using probiotic lactobacilli strains. Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 were shown to have completely inhibited C. glabrata biofilms and the results were corroborated by scanning electron microscopy (SEM), which revealed scanty structures of the mixed biofilms of C. glabrata and probiotic lactobacilli strains. In addition, biofilm-related C. glabrata genes EPA6 and YAK1 were downregulated in response to the probiotic lactobacilli challenges. The present study suggested that probiotic L. rhamnosus GR-1 and L. reuteri RC-14 strains inhibited C. glabrata biofilm by partially impeding the adherence of yeast cells and the effect might be contributed by the secretory compounds produced by these probiotic lactobacilli strains. Further investigations are required to examine and identify the biofilm inhibitory compounds and the mechanism of probiotic actions of these lactobacilli strains. |
| first_indexed | 2025-11-15T09:39:37Z |
| format | Article |
| id | upm-38010 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:39:37Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-380102015-12-18T05:42:35Z http://psasir.upm.edu.my/id/eprint/38010/ In vitro modulation of probiotic bacteria on the biofilm of Candida glabrata Chew, Shu Yih Cheah, Yoke Kqueen Seow, Heng Fong Sandai, Doblin Than, Leslie Thian Lung A conspicuous new concept of pathogens living as the microbial societies in the human host rather than free planktonic cells has raised considerable concerns among scientists and clinicians. Fungal biofilms are communities of cells that possess distinct characteristic such as increased resistance to the immune defence and antimycotic agents in comparison to their planktonic cells counterpart. Therefore, inhibition of the biofilm may represent a new paradigm for antifungal development. In this study, we aim to evaluate the in vitro modulation of vulvovaginal candidiasis (VVC)-causing Candida glabrata biofilms using probiotic lactobacilli strains. Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 were shown to have completely inhibited C. glabrata biofilms and the results were corroborated by scanning electron microscopy (SEM), which revealed scanty structures of the mixed biofilms of C. glabrata and probiotic lactobacilli strains. In addition, biofilm-related C. glabrata genes EPA6 and YAK1 were downregulated in response to the probiotic lactobacilli challenges. The present study suggested that probiotic L. rhamnosus GR-1 and L. reuteri RC-14 strains inhibited C. glabrata biofilm by partially impeding the adherence of yeast cells and the effect might be contributed by the secretory compounds produced by these probiotic lactobacilli strains. Further investigations are required to examine and identify the biofilm inhibitory compounds and the mechanism of probiotic actions of these lactobacilli strains. Elsevier 2015-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/38010/1/In%20vitro%20modulation%20of%20probiotic%20bacteria%20on%20the%20biofilm%20of%20Candida%20glabrata.pdf Chew, Shu Yih and Cheah, Yoke Kqueen and Seow, Heng Fong and Sandai, Doblin and Than, Leslie Thian Lung (2015) In vitro modulation of probiotic bacteria on the biofilm of Candida glabrata. Anaerobe, 34. pp. 132-138. ISSN 1075-9964; ESSN: 1095-8274 http://www.sciencedirect.com/science/article/pii/S1075996415300275 10.1016/j.anaerobe.2015.05.009 |
| spellingShingle | Chew, Shu Yih Cheah, Yoke Kqueen Seow, Heng Fong Sandai, Doblin Than, Leslie Thian Lung In vitro modulation of probiotic bacteria on the biofilm of Candida glabrata |
| title | In vitro modulation of probiotic bacteria on the biofilm of Candida glabrata |
| title_full | In vitro modulation of probiotic bacteria on the biofilm of Candida glabrata |
| title_fullStr | In vitro modulation of probiotic bacteria on the biofilm of Candida glabrata |
| title_full_unstemmed | In vitro modulation of probiotic bacteria on the biofilm of Candida glabrata |
| title_short | In vitro modulation of probiotic bacteria on the biofilm of Candida glabrata |
| title_sort | in vitro modulation of probiotic bacteria on the biofilm of candida glabrata |
| url | http://psasir.upm.edu.my/id/eprint/38010/ http://psasir.upm.edu.my/id/eprint/38010/ http://psasir.upm.edu.my/id/eprint/38010/ http://psasir.upm.edu.my/id/eprint/38010/1/In%20vitro%20modulation%20of%20probiotic%20bacteria%20on%20the%20biofilm%20of%20Candida%20glabrata.pdf |