Viral persistence in colorectal cancer cells infected by Newcastle disease virus
Background Newcastle disease virus (NDV), a single-stranded RNA virus of the family Paramyxoviridae, is a candidate virotherapy agent in cancer treatment. Promising responses were observed in clinical studies. Despite its high potential, the possibility of the virus to develop a persistent form of...
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| Format: | Article |
| Language: | English |
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BioMed Central
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35987/ http://psasir.upm.edu.my/id/eprint/35987/1/Viral%20persistence%20in%20colorectal%20cancer%20cells.pdf |
| _version_ | 1848848207333818368 |
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| author | Chia, Suet Lin Yusoff, Khatijah Shafee, Norazizah |
| author_facet | Chia, Suet Lin Yusoff, Khatijah Shafee, Norazizah |
| author_sort | Chia, Suet Lin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background
Newcastle disease virus (NDV), a single-stranded RNA virus of the family Paramyxoviridae, is a candidate virotherapy agent in cancer treatment. Promising responses were observed in clinical studies. Despite its high potential, the possibility of the virus to develop a persistent form of infection in cancer cells has not been investigated. Occurrence of persistent infection by NDV in cancer cells may cause the cells to be less susceptible to the virus killing. This would give rise to a population of cancer cells that remains viable and resistant to treatment.
Results
During infection experiment in a series of colorectal cancer cell lines, we adventitiously observed a development of persistent infection by NDV in SW480 cells, but not in other cell lines tested. This cell population, designated as SW480P, showed resistancy towards NDV killing in a re-infection experiment. The SW480P cells retained NDV genome and produced virus progeny with reduced plaque forming ability.
Conclusion
These observations showed that NDV could develop persistent infection in cancer cells and this factor needs to be taken into consideration when using NDV in clinical settings.
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| first_indexed | 2025-11-15T09:30:50Z |
| format | Article |
| id | upm-35987 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:30:50Z |
| publishDate | 2014 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-359872016-02-12T07:19:56Z http://psasir.upm.edu.my/id/eprint/35987/ Viral persistence in colorectal cancer cells infected by Newcastle disease virus Chia, Suet Lin Yusoff, Khatijah Shafee, Norazizah Background Newcastle disease virus (NDV), a single-stranded RNA virus of the family Paramyxoviridae, is a candidate virotherapy agent in cancer treatment. Promising responses were observed in clinical studies. Despite its high potential, the possibility of the virus to develop a persistent form of infection in cancer cells has not been investigated. Occurrence of persistent infection by NDV in cancer cells may cause the cells to be less susceptible to the virus killing. This would give rise to a population of cancer cells that remains viable and resistant to treatment. Results During infection experiment in a series of colorectal cancer cell lines, we adventitiously observed a development of persistent infection by NDV in SW480 cells, but not in other cell lines tested. This cell population, designated as SW480P, showed resistancy towards NDV killing in a re-infection experiment. The SW480P cells retained NDV genome and produced virus progeny with reduced plaque forming ability. Conclusion These observations showed that NDV could develop persistent infection in cancer cells and this factor needs to be taken into consideration when using NDV in clinical settings. BioMed Central 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/35987/1/Viral%20persistence%20in%20colorectal%20cancer%20cells.pdf Chia, Suet Lin and Yusoff, Khatijah and Shafee, Norazizah (2014) Viral persistence in colorectal cancer cells infected by Newcastle disease virus. Virology Journal, 11. art. no. 91. pp. 1-8. ISSN 1743-422X http://virologyj.biomedcentral.com/articles/10.1186/1743-422X-11-91 10.1186/1743-422X-11-91 |
| spellingShingle | Chia, Suet Lin Yusoff, Khatijah Shafee, Norazizah Viral persistence in colorectal cancer cells infected by Newcastle disease virus |
| title | Viral persistence in colorectal cancer cells infected by Newcastle disease virus |
| title_full | Viral persistence in colorectal cancer cells infected by Newcastle disease virus |
| title_fullStr | Viral persistence in colorectal cancer cells infected by Newcastle disease virus |
| title_full_unstemmed | Viral persistence in colorectal cancer cells infected by Newcastle disease virus |
| title_short | Viral persistence in colorectal cancer cells infected by Newcastle disease virus |
| title_sort | viral persistence in colorectal cancer cells infected by newcastle disease virus |
| url | http://psasir.upm.edu.my/id/eprint/35987/ http://psasir.upm.edu.my/id/eprint/35987/ http://psasir.upm.edu.my/id/eprint/35987/ http://psasir.upm.edu.my/id/eprint/35987/1/Viral%20persistence%20in%20colorectal%20cancer%20cells.pdf |