Somatic embryogenesis from leaf and shoot tip explants of Jatropha curcas L.
Jatropha curcas is a non-edible oil plant species and belongs to Euphorbiaceae family. It is gaining the importance as potential renewable feed-stock for biodiesel production. Here in the present study we developed an efficient micropropagation protocol through somatic embryogenesis from leaf and sh...
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| Format: | Article |
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Indian Society for Education and Environment
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35417/ |
| _version_ | 1848848049059659776 |
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| author | Medipally, Srikanth Reddy Naresh, Baja Kumar, S. Manoj Md. Yusoff, Fatimah Prathibha Devi, |
| author_facet | Medipally, Srikanth Reddy Naresh, Baja Kumar, S. Manoj Md. Yusoff, Fatimah Prathibha Devi, |
| author_sort | Medipally, Srikanth Reddy |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Jatropha curcas is a non-edible oil plant species and belongs to Euphorbiaceae family. It is gaining the importance as potential renewable feed-stock for biodiesel production. Here in the present study we developed an efficient micropropagation protocol through somatic embryogenesis from leaf and shoot tip explants of Jatropha curcas. Murashigae-Skoog medium supplemented with 2, 4-D (0.5mg/l) and benzylaminopurine (5mg/l) resulted in the production of green embryogenic callus with clearly differentiated somatic embryos with a frequency of plant conversion rate from shoot tip and leaf explants was 51 ± 0.9 and 54 ± 0.6 respectively. The regenerated shoots were rooted on root induction medium, transplanted to pots and later transferred to field. The survival percentage of the transplanted plants was 33±0.1 for shoot tip and 24±0.7 for leaf explant. Therefore, this protocol could be useful for micropropagation of Jatropha curcas as effective feed-stock towards biodiesel production. |
| first_indexed | 2025-11-15T09:28:19Z |
| format | Article |
| id | upm-35417 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:28:19Z |
| publishDate | 2014 |
| publisher | Indian Society for Education and Environment |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-354172016-01-11T01:33:49Z http://psasir.upm.edu.my/id/eprint/35417/ Somatic embryogenesis from leaf and shoot tip explants of Jatropha curcas L. Medipally, Srikanth Reddy Naresh, Baja Kumar, S. Manoj Md. Yusoff, Fatimah Prathibha Devi, Jatropha curcas is a non-edible oil plant species and belongs to Euphorbiaceae family. It is gaining the importance as potential renewable feed-stock for biodiesel production. Here in the present study we developed an efficient micropropagation protocol through somatic embryogenesis from leaf and shoot tip explants of Jatropha curcas. Murashigae-Skoog medium supplemented with 2, 4-D (0.5mg/l) and benzylaminopurine (5mg/l) resulted in the production of green embryogenic callus with clearly differentiated somatic embryos with a frequency of plant conversion rate from shoot tip and leaf explants was 51 ± 0.9 and 54 ± 0.6 respectively. The regenerated shoots were rooted on root induction medium, transplanted to pots and later transferred to field. The survival percentage of the transplanted plants was 33±0.1 for shoot tip and 24±0.7 for leaf explant. Therefore, this protocol could be useful for micropropagation of Jatropha curcas as effective feed-stock towards biodiesel production. Indian Society for Education and Environment 2014-11 Article PeerReviewed Medipally, Srikanth Reddy and Naresh, Baja and Kumar, S. Manoj and Md. Yusoff, Fatimah and Prathibha Devi, (2014) Somatic embryogenesis from leaf and shoot tip explants of Jatropha curcas L. Indian Journal of Science and Technology, 7 (11). pp. 1842-1846. ISSN 0974-6846; ESSN: 0974-5645 http://www.indjst.org/index.php/indjst/article/view/47813 |
| spellingShingle | Medipally, Srikanth Reddy Naresh, Baja Kumar, S. Manoj Md. Yusoff, Fatimah Prathibha Devi, Somatic embryogenesis from leaf and shoot tip explants of Jatropha curcas L. |
| title | Somatic embryogenesis from leaf and shoot tip explants of Jatropha curcas L. |
| title_full | Somatic embryogenesis from leaf and shoot tip explants of Jatropha curcas L. |
| title_fullStr | Somatic embryogenesis from leaf and shoot tip explants of Jatropha curcas L. |
| title_full_unstemmed | Somatic embryogenesis from leaf and shoot tip explants of Jatropha curcas L. |
| title_short | Somatic embryogenesis from leaf and shoot tip explants of Jatropha curcas L. |
| title_sort | somatic embryogenesis from leaf and shoot tip explants of jatropha curcas l. |
| url | http://psasir.upm.edu.my/id/eprint/35417/ http://psasir.upm.edu.my/id/eprint/35417/ |