RNA interference of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1 and ACO2) genes expression prolongs the shelf life of Eksotika (Carica papaya L.) papaya fruit
The purpose of this study was to evaluate the effectiveness of using RNA interference in down regulating the expression of 1-aminocyclopropane-1-carboxylic acid oxidase gene in Eksotika papaya. One-month old embryogenic calli were separately transformed with Agrobacterium strain LBA 4404 harbouring...
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| Format: | Article |
| Language: | English |
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MDPI
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35286/ http://psasir.upm.edu.my/id/eprint/35286/1/RNA%20interference%20of%201.pdf |
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| author | Sekeli, Rogayah Abdullah, Janna Ong Namasivayam, Parameswari Muda, Pauziah Abu Bakar, Umi Kalsom Yeong, Wee Chien Pillai, Vilasini |
| author_facet | Sekeli, Rogayah Abdullah, Janna Ong Namasivayam, Parameswari Muda, Pauziah Abu Bakar, Umi Kalsom Yeong, Wee Chien Pillai, Vilasini |
| author_sort | Sekeli, Rogayah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The purpose of this study was to evaluate the effectiveness of using RNA interference in down regulating the expression of 1-aminocyclopropane-1-carboxylic acid oxidase gene in Eksotika papaya. One-month old embryogenic calli were separately transformed with Agrobacterium strain LBA 4404 harbouring the three different RNAi pOpOff2 constructs bearing the 1-aminocyclopropane-1-carboxylic acid oxidase gene. A total of 176 putative transformed lines were produced from 15,000 calli transformed, selected, then regenerated on medium supplemented with kanamycin. Integration and expression of the targeted gene in putatively transformed lines were verified by PCR and real-time RT-PCR. Confined field evaluation of a total of 31 putative transgenic lines planted showed a knockdown expression of the targeted ACO1 and ACO2 genes in 13 lines, which required more than 8 days to achieve the full yellow colour (Index 6). Fruits harvested from lines pRNAiACO2 L2-9 and pRNAiACO1 L2 exhibited about 20 and 14 days extended post-harvest shelf life to reach Index 6, respectively. The total soluble solids contents of the fruits ranged from 11 to 14° Brix, a range similar to fruits from non-transformed, wild type seed-derived plants. |
| first_indexed | 2025-11-15T09:27:44Z |
| format | Article |
| id | upm-35286 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:27:44Z |
| publishDate | 2014 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-352862016-01-27T03:56:21Z http://psasir.upm.edu.my/id/eprint/35286/ RNA interference of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1 and ACO2) genes expression prolongs the shelf life of Eksotika (Carica papaya L.) papaya fruit Sekeli, Rogayah Abdullah, Janna Ong Namasivayam, Parameswari Muda, Pauziah Abu Bakar, Umi Kalsom Yeong, Wee Chien Pillai, Vilasini The purpose of this study was to evaluate the effectiveness of using RNA interference in down regulating the expression of 1-aminocyclopropane-1-carboxylic acid oxidase gene in Eksotika papaya. One-month old embryogenic calli were separately transformed with Agrobacterium strain LBA 4404 harbouring the three different RNAi pOpOff2 constructs bearing the 1-aminocyclopropane-1-carboxylic acid oxidase gene. A total of 176 putative transformed lines were produced from 15,000 calli transformed, selected, then regenerated on medium supplemented with kanamycin. Integration and expression of the targeted gene in putatively transformed lines were verified by PCR and real-time RT-PCR. Confined field evaluation of a total of 31 putative transgenic lines planted showed a knockdown expression of the targeted ACO1 and ACO2 genes in 13 lines, which required more than 8 days to achieve the full yellow colour (Index 6). Fruits harvested from lines pRNAiACO2 L2-9 and pRNAiACO1 L2 exhibited about 20 and 14 days extended post-harvest shelf life to reach Index 6, respectively. The total soluble solids contents of the fruits ranged from 11 to 14° Brix, a range similar to fruits from non-transformed, wild type seed-derived plants. MDPI 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/35286/1/RNA%20interference%20of%201.pdf Sekeli, Rogayah and Abdullah, Janna Ong and Namasivayam, Parameswari and Muda, Pauziah and Abu Bakar, Umi Kalsom and Yeong, Wee Chien and Pillai, Vilasini (2014) RNA interference of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1 and ACO2) genes expression prolongs the shelf life of Eksotika (Carica papaya L.) papaya fruit. Molecules, 19 (6). pp. 8350-8362. ISSN 1420-3049 http://www.mdpi.com/1420-3049/19/6/8350 10.3390/molecules19068350 |
| spellingShingle | Sekeli, Rogayah Abdullah, Janna Ong Namasivayam, Parameswari Muda, Pauziah Abu Bakar, Umi Kalsom Yeong, Wee Chien Pillai, Vilasini RNA interference of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1 and ACO2) genes expression prolongs the shelf life of Eksotika (Carica papaya L.) papaya fruit |
| title | RNA interference of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1 and ACO2) genes expression prolongs the shelf life of Eksotika (Carica papaya L.) papaya fruit |
| title_full | RNA interference of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1 and ACO2) genes expression prolongs the shelf life of Eksotika (Carica papaya L.) papaya fruit |
| title_fullStr | RNA interference of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1 and ACO2) genes expression prolongs the shelf life of Eksotika (Carica papaya L.) papaya fruit |
| title_full_unstemmed | RNA interference of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1 and ACO2) genes expression prolongs the shelf life of Eksotika (Carica papaya L.) papaya fruit |
| title_short | RNA interference of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1 and ACO2) genes expression prolongs the shelf life of Eksotika (Carica papaya L.) papaya fruit |
| title_sort | rna interference of 1-aminocyclopropane-1-carboxylic acid oxidase (aco1 and aco2) genes expression prolongs the shelf life of eksotika (carica papaya l.) papaya fruit |
| url | http://psasir.upm.edu.my/id/eprint/35286/ http://psasir.upm.edu.my/id/eprint/35286/ http://psasir.upm.edu.my/id/eprint/35286/ http://psasir.upm.edu.my/id/eprint/35286/1/RNA%20interference%20of%201.pdf |