Primary culture of human amniotic fluid cells harvested from full-term pregnancies
Heterogeneous cells contained in human amniotic fluid (AF) are believed to hold therapeutic potential. Mid-term AF has been discovered to harbor high potency stem cell population. However, it is not clear whether AF of full-term pregnancy contains this type of cells. In an effort to explore this pos...
| Main Authors: | , , , , |
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| Format: | Article |
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Tissue Engineering and Regenerative Medicine Society of Malaysia
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35050/ |
| _version_ | 1848847946128293888 |
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| author | Joharry , Muhammad Khair Karuppiah, Thilakavathy Rejali, Zulida Yazid, Mohd Nazri Nordin, Norshariza |
| author_facet | Joharry , Muhammad Khair Karuppiah, Thilakavathy Rejali, Zulida Yazid, Mohd Nazri Nordin, Norshariza |
| author_sort | Joharry , Muhammad Khair |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Heterogeneous cells contained in human amniotic fluid (AF) are believed to hold therapeutic potential. Mid-term AF has been discovered to harbor high potency stem cell population. However, it is not clear whether AF of full-term pregnancy contains this type of cells. In an effort to explore this possibility, primary culture of AF harvested from full-term pregnancies or during deliveries needs to be established. Here, we aimed to culture and propagate AF cells of 38-40 weeks gestation collected during normal (ARM) and cesarean section (C-Sect) deliveries. The time taken for both cells to reach confluency and the cell morphology were compared and evaluated. We found C-Sect samples took shorter time to confluent compared to ARM samples in Amniomax with both exhibiting similar cell morphology. These results prove the presence of viability cells residing in full-term AF, thus, giving us high hope for the existence of stem cell population in the fluid, which is normally discarded.
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| first_indexed | 2025-11-15T09:26:41Z |
| format | Article |
| id | upm-35050 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:26:41Z |
| publishDate | 2014 |
| publisher | Tissue Engineering and Regenerative Medicine Society of Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-350502015-12-29T07:38:33Z http://psasir.upm.edu.my/id/eprint/35050/ Primary culture of human amniotic fluid cells harvested from full-term pregnancies Joharry , Muhammad Khair Karuppiah, Thilakavathy Rejali, Zulida Yazid, Mohd Nazri Nordin, Norshariza Heterogeneous cells contained in human amniotic fluid (AF) are believed to hold therapeutic potential. Mid-term AF has been discovered to harbor high potency stem cell population. However, it is not clear whether AF of full-term pregnancy contains this type of cells. In an effort to explore this possibility, primary culture of AF harvested from full-term pregnancies or during deliveries needs to be established. Here, we aimed to culture and propagate AF cells of 38-40 weeks gestation collected during normal (ARM) and cesarean section (C-Sect) deliveries. The time taken for both cells to reach confluency and the cell morphology were compared and evaluated. We found C-Sect samples took shorter time to confluent compared to ARM samples in Amniomax with both exhibiting similar cell morphology. These results prove the presence of viability cells residing in full-term AF, thus, giving us high hope for the existence of stem cell population in the fluid, which is normally discarded. Tissue Engineering and Regenerative Medicine Society of Malaysia 2014-12 Article PeerReviewed Joharry , Muhammad Khair and Karuppiah, Thilakavathy and Rejali, Zulida and Yazid, Mohd Nazri and Nordin, Norshariza (2014) Primary culture of human amniotic fluid cells harvested from full-term pregnancies. Regenerative Research, 3 (2). pp. 120-121. ISSN 2232-0822 http://www.regres.tesma.org.my/index-7.html |
| spellingShingle | Joharry , Muhammad Khair Karuppiah, Thilakavathy Rejali, Zulida Yazid, Mohd Nazri Nordin, Norshariza Primary culture of human amniotic fluid cells harvested from full-term pregnancies |
| title | Primary culture of human amniotic fluid cells harvested from full-term pregnancies |
| title_full | Primary culture of human amniotic fluid cells harvested from full-term pregnancies |
| title_fullStr | Primary culture of human amniotic fluid cells harvested from full-term pregnancies |
| title_full_unstemmed | Primary culture of human amniotic fluid cells harvested from full-term pregnancies |
| title_short | Primary culture of human amniotic fluid cells harvested from full-term pregnancies |
| title_sort | primary culture of human amniotic fluid cells harvested from full-term pregnancies |
| url | http://psasir.upm.edu.my/id/eprint/35050/ http://psasir.upm.edu.my/id/eprint/35050/ |