Enhanced antigenicity of Nipah virus nucleocapsid protein displayed on hepatitis B core antigen
Display of the nucleocapsid protein (NP) of Nipah virus (NiV) on the hepatitis B core (HBc) particle enhances the antigenicity of the NP. In addition, the NP fused to the amino-teminus (NP401-532 HBc) and immunodominant loop of HBc (HBc79.80NP4O1'S31) was shown to be less sensitive to proteinas...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/31947/ http://psasir.upm.edu.my/id/eprint/31947/1/31947.pdf |
| _version_ | 1848847082629103616 |
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| author | Yap, Wei Boon Tey, Beng Ti Mohammed Alitheen, Noorjahan Banu Tan, Wen Siang |
| author_facet | Yap, Wei Boon Tey, Beng Ti Mohammed Alitheen, Noorjahan Banu Tan, Wen Siang |
| author_sort | Yap, Wei Boon |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Display of the nucleocapsid protein (NP) of Nipah virus (NiV) on the hepatitis B core (HBc) particle enhances the antigenicity of the NP. In addition, the NP fused to the amino-teminus (NP401-532 HBc) and immunodominant loop of HBc (HBc79.80NP4O1'S31) was shown to be less sensitive to proteinase K. Some of the proteinase K treated NP401.32HBc and HBc79.80NP,O].s32 remained intact and antigenic. These results indicated two important implications, (1) the use of HBc virus -like particle as a carrier platform for foreign epitopes, (2 ) the use of NP401.532HBc as a diagnostic reagent for NiV infection. |
| first_indexed | 2025-11-15T09:12:57Z |
| format | Conference or Workshop Item |
| id | upm-31947 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:12:57Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-319472017-01-09T09:33:48Z http://psasir.upm.edu.my/id/eprint/31947/ Enhanced antigenicity of Nipah virus nucleocapsid protein displayed on hepatitis B core antigen Yap, Wei Boon Tey, Beng Ti Mohammed Alitheen, Noorjahan Banu Tan, Wen Siang Display of the nucleocapsid protein (NP) of Nipah virus (NiV) on the hepatitis B core (HBc) particle enhances the antigenicity of the NP. In addition, the NP fused to the amino-teminus (NP401-532 HBc) and immunodominant loop of HBc (HBc79.80NP4O1'S31) was shown to be less sensitive to proteinase K. Some of the proteinase K treated NP401.32HBc and HBc79.80NP,O].s32 remained intact and antigenic. These results indicated two important implications, (1) the use of HBc virus -like particle as a carrier platform for foreign epitopes, (2 ) the use of NP401.532HBc as a diagnostic reagent for NiV infection. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/31947/1/31947.pdf Yap, Wei Boon and Tey, Beng Ti and Mohammed Alitheen, Noorjahan Banu and Tan, Wen Siang (2012) Enhanced antigenicity of Nipah virus nucleocapsid protein displayed on hepatitis B core antigen. In: International Conference on One Health and 24th VAM Congress 2012, 21-23 Sept. 2012, Marriott Putrajaya. (pp. 324-326). |
| spellingShingle | Yap, Wei Boon Tey, Beng Ti Mohammed Alitheen, Noorjahan Banu Tan, Wen Siang Enhanced antigenicity of Nipah virus nucleocapsid protein displayed on hepatitis B core antigen |
| title | Enhanced antigenicity of Nipah virus nucleocapsid protein displayed on hepatitis B core antigen |
| title_full | Enhanced antigenicity of Nipah virus nucleocapsid protein displayed on hepatitis B core antigen |
| title_fullStr | Enhanced antigenicity of Nipah virus nucleocapsid protein displayed on hepatitis B core antigen |
| title_full_unstemmed | Enhanced antigenicity of Nipah virus nucleocapsid protein displayed on hepatitis B core antigen |
| title_short | Enhanced antigenicity of Nipah virus nucleocapsid protein displayed on hepatitis B core antigen |
| title_sort | enhanced antigenicity of nipah virus nucleocapsid protein displayed on hepatitis b core antigen |
| url | http://psasir.upm.edu.my/id/eprint/31947/ http://psasir.upm.edu.my/id/eprint/31947/1/31947.pdf |