Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons
Thymoquinone (TQ), a bioactive constituent of Nigella sativa Linn (N. sativa) has demonstrated several neuropharmacological attributes. In the present study, the neuroprotective properties of TQ were investigated by studying its anti-apoptotic potential to diminish β-amyloid peptide 1–40 sequence (A...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2013
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/30729/ http://psasir.upm.edu.my/id/eprint/30729/1/Thymoquinone%20prevents%20%CE%B2.pdf |
| _version_ | 1848846760921792512 |
|---|---|
| author | Ismail, Norsharina Ismail, Maznah Mazlan, Musalmah Abd Latiff, Latiffah Imam, Mustapha Umar Iqbal, Shahid Azmi, Nur Hanisah Abd Ghafar, Siti Aisyah Kim, Wei Chan |
| author_facet | Ismail, Norsharina Ismail, Maznah Mazlan, Musalmah Abd Latiff, Latiffah Imam, Mustapha Umar Iqbal, Shahid Azmi, Nur Hanisah Abd Ghafar, Siti Aisyah Kim, Wei Chan |
| author_sort | Ismail, Norsharina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Thymoquinone (TQ), a bioactive constituent of Nigella sativa Linn (N. sativa) has demonstrated several neuropharmacological attributes. In the present study, the neuroprotective properties of TQ were investigated by studying its anti-apoptotic potential to diminish β-amyloid peptide 1–40 sequence (Aβ1–40)-induced neuronal cell death in primary cultured cerebellar granule neurons (CGNs). The effects of TQ against Aβ1–40-induced neurotoxicity, morphological damages, DNA condensation, the generation of reactive oxygen species, and caspase-3, -8, and -9 activation were investigated. Pretreatment of CGNs with TQ (0.1 and 1 μM) and subsequent exposure to 10 μM Aβ1–40 protected the CGNs against the neurotoxic effects of the latter. In addition, the CGNs were better preserved with intact cell bodies, extensive neurite networks, a loss of condensed chromatin and less free radical generation than those exposed to Aβ1–40 alone. TQ pretreatment inhibited Aβ1–40-induced apoptosis of CGNs via both extrinsic and intrinsic caspase pathways. Thus, the findings of this study suggest that TQ may prevent neurotoxicity and Aβ1–40-induced apoptosis. TQ is, therefore, worth studying further for its potential to reduce the risks of developing Alzheimer’s disease. |
| first_indexed | 2025-11-15T09:07:50Z |
| format | Article |
| id | upm-30729 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:07:50Z |
| publishDate | 2013 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-307292015-09-22T03:06:22Z http://psasir.upm.edu.my/id/eprint/30729/ Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons Ismail, Norsharina Ismail, Maznah Mazlan, Musalmah Abd Latiff, Latiffah Imam, Mustapha Umar Iqbal, Shahid Azmi, Nur Hanisah Abd Ghafar, Siti Aisyah Kim, Wei Chan Thymoquinone (TQ), a bioactive constituent of Nigella sativa Linn (N. sativa) has demonstrated several neuropharmacological attributes. In the present study, the neuroprotective properties of TQ were investigated by studying its anti-apoptotic potential to diminish β-amyloid peptide 1–40 sequence (Aβ1–40)-induced neuronal cell death in primary cultured cerebellar granule neurons (CGNs). The effects of TQ against Aβ1–40-induced neurotoxicity, morphological damages, DNA condensation, the generation of reactive oxygen species, and caspase-3, -8, and -9 activation were investigated. Pretreatment of CGNs with TQ (0.1 and 1 μM) and subsequent exposure to 10 μM Aβ1–40 protected the CGNs against the neurotoxic effects of the latter. In addition, the CGNs were better preserved with intact cell bodies, extensive neurite networks, a loss of condensed chromatin and less free radical generation than those exposed to Aβ1–40 alone. TQ pretreatment inhibited Aβ1–40-induced apoptosis of CGNs via both extrinsic and intrinsic caspase pathways. Thus, the findings of this study suggest that TQ may prevent neurotoxicity and Aβ1–40-induced apoptosis. TQ is, therefore, worth studying further for its potential to reduce the risks of developing Alzheimer’s disease. Springer 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30729/1/Thymoquinone%20prevents%20%CE%B2.pdf Ismail, Norsharina and Ismail, Maznah and Mazlan, Musalmah and Abd Latiff, Latiffah and Imam, Mustapha Umar and Iqbal, Shahid and Azmi, Nur Hanisah and Abd Ghafar, Siti Aisyah and Kim, Wei Chan (2013) Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons. Cellular and Molecular Neurobiology, 33 (8). pp. 1159-1169. ISSN 0272-4340; ESSN: 1573-6830 10.1007/s10571-013-9982-z |
| spellingShingle | Ismail, Norsharina Ismail, Maznah Mazlan, Musalmah Abd Latiff, Latiffah Imam, Mustapha Umar Iqbal, Shahid Azmi, Nur Hanisah Abd Ghafar, Siti Aisyah Kim, Wei Chan Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons |
| title | Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons |
| title_full | Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons |
| title_fullStr | Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons |
| title_full_unstemmed | Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons |
| title_short | Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons |
| title_sort | thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons |
| url | http://psasir.upm.edu.my/id/eprint/30729/ http://psasir.upm.edu.my/id/eprint/30729/ http://psasir.upm.edu.my/id/eprint/30729/1/Thymoquinone%20prevents%20%CE%B2.pdf |