Development of a fluorescence resonance energy transfer (FRET)-based DNA biosensor for detection of synthetic oligonucleotide of Ganoderma boninense
An optical DNA biosensor based on fluorescence resonance energy transfer (FRET) utilizing synthesized quantum dot (QD) has been developed for the detection of specific-sequence of DNA for Ganoderma boninense, an oil palm pathogen. Modified QD that contained carboxylic groups was conjugated with a si...
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| Format: | Article |
| Language: | English |
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MDPI
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/30302/ http://psasir.upm.edu.my/id/eprint/30302/1/30302.pdf |
| _version_ | 1848846638883274752 |
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| author | Mohd Bakhori, Noremylia Yusof, Nor Azah Abdullah, Abdul Halim Hussein, Mohd Zobir |
| author_facet | Mohd Bakhori, Noremylia Yusof, Nor Azah Abdullah, Abdul Halim Hussein, Mohd Zobir |
| author_sort | Mohd Bakhori, Noremylia |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An optical DNA biosensor based on fluorescence resonance energy transfer (FRET) utilizing synthesized quantum dot (QD) has been developed for the detection of specific-sequence of DNA for Ganoderma boninense, an oil palm pathogen. Modified QD that contained carboxylic groups was conjugated with a single-stranded DNA probe (ssDNA) via amide-linkage. Hybridization of the target DNA with conjugated QD-ssDNA and reporter probe labeled with Cy5 allows for the detection of related synthetic DNA sequence of Ganoderma boninense gene based on FRET signals. Detection of FRET emission before and after hybridization was confirmed through the capability of the system to produce FRET at 680 nm for hybridized sandwich with complementary target DNA. No FRET emission was observed for non-complementary system. Hybridization time, temperature and effect of different concentration of target DNA were studied in order to optimize the developed system. The developed biosensor has shown high sensitivity with detection limit of 3.55 × 10−9 M. TEM results show that the particle size of QD varies in the range between 5 to 8 nm after ligand modification and conjugation with ssDNA. This approach is capable of providing a simple, rapid and sensitive method for detection of related synthetic DNA sequence of Ganoderma boninense. |
| first_indexed | 2025-11-15T09:05:54Z |
| format | Article |
| id | upm-30302 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:05:54Z |
| publishDate | 2013 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-303022017-11-01T09:15:01Z http://psasir.upm.edu.my/id/eprint/30302/ Development of a fluorescence resonance energy transfer (FRET)-based DNA biosensor for detection of synthetic oligonucleotide of Ganoderma boninense Mohd Bakhori, Noremylia Yusof, Nor Azah Abdullah, Abdul Halim Hussein, Mohd Zobir An optical DNA biosensor based on fluorescence resonance energy transfer (FRET) utilizing synthesized quantum dot (QD) has been developed for the detection of specific-sequence of DNA for Ganoderma boninense, an oil palm pathogen. Modified QD that contained carboxylic groups was conjugated with a single-stranded DNA probe (ssDNA) via amide-linkage. Hybridization of the target DNA with conjugated QD-ssDNA and reporter probe labeled with Cy5 allows for the detection of related synthetic DNA sequence of Ganoderma boninense gene based on FRET signals. Detection of FRET emission before and after hybridization was confirmed through the capability of the system to produce FRET at 680 nm for hybridized sandwich with complementary target DNA. No FRET emission was observed for non-complementary system. Hybridization time, temperature and effect of different concentration of target DNA were studied in order to optimize the developed system. The developed biosensor has shown high sensitivity with detection limit of 3.55 × 10−9 M. TEM results show that the particle size of QD varies in the range between 5 to 8 nm after ligand modification and conjugation with ssDNA. This approach is capable of providing a simple, rapid and sensitive method for detection of related synthetic DNA sequence of Ganoderma boninense. MDPI 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30302/1/30302.pdf Mohd Bakhori, Noremylia and Yusof, Nor Azah and Abdullah, Abdul Halim and Hussein, Mohd Zobir (2013) Development of a fluorescence resonance energy transfer (FRET)-based DNA biosensor for detection of synthetic oligonucleotide of Ganoderma boninense. Biosensors, 3 (4). pp. 419-428. ISSN 2079-6374 http://www.mdpi.com/2079-6374/3/4/419 10.3390/bios3040419 |
| spellingShingle | Mohd Bakhori, Noremylia Yusof, Nor Azah Abdullah, Abdul Halim Hussein, Mohd Zobir Development of a fluorescence resonance energy transfer (FRET)-based DNA biosensor for detection of synthetic oligonucleotide of Ganoderma boninense |
| title | Development of a fluorescence resonance energy transfer (FRET)-based DNA biosensor for detection of synthetic oligonucleotide of Ganoderma boninense |
| title_full | Development of a fluorescence resonance energy transfer (FRET)-based DNA biosensor for detection of synthetic oligonucleotide of Ganoderma boninense |
| title_fullStr | Development of a fluorescence resonance energy transfer (FRET)-based DNA biosensor for detection of synthetic oligonucleotide of Ganoderma boninense |
| title_full_unstemmed | Development of a fluorescence resonance energy transfer (FRET)-based DNA biosensor for detection of synthetic oligonucleotide of Ganoderma boninense |
| title_short | Development of a fluorescence resonance energy transfer (FRET)-based DNA biosensor for detection of synthetic oligonucleotide of Ganoderma boninense |
| title_sort | development of a fluorescence resonance energy transfer (fret)-based dna biosensor for detection of synthetic oligonucleotide of ganoderma boninense |
| url | http://psasir.upm.edu.my/id/eprint/30302/ http://psasir.upm.edu.my/id/eprint/30302/ http://psasir.upm.edu.my/id/eprint/30302/ http://psasir.upm.edu.my/id/eprint/30302/1/30302.pdf |