Enhancement of protease production by the optimization of Bacillus subtilis culture medium.
Aims: Traditionally, crustacean wastes have been managed by using acid and alkali which leads to major environmental issue. However, over the recent years microbial fermentation has gained its way whereby producing similar effects as chemical treatment and a higher quality product can be obtained....
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Malaysian Society for Microbiology
2013
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/29997/ http://psasir.upm.edu.my/id/eprint/29997/1/Enhancement%20of%20protease%20production%20by%20the%20optimization%20of%20Bacillus%20subtilis%20culture%20medium.pdf |
| _version_ | 1848846554221248512 |
|---|---|
| author | Cheong, Jee Yin Mustafa, Muskhazli Abd Aziz, Nor Azwady Go, Rusea Ahmad Adli, Azleen |
| author_facet | Cheong, Jee Yin Mustafa, Muskhazli Abd Aziz, Nor Azwady Go, Rusea Ahmad Adli, Azleen |
| author_sort | Cheong, Jee Yin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aims: Traditionally, crustacean wastes have been managed by using acid and alkali which leads to major environmental
issue. However, over the recent years microbial fermentation has gained its way whereby producing similar effects as chemical treatment and a higher quality product can be obtained. Extracellular protease from Bacillus subtilis was used further by optimizing its culture medium to enhance protease production.
Methodology and Results: The culture media was optimized with 4 various sources; Shrimp Crab Shell Powder
(SCSP), nitrogen sources, inorganic salts, and carbon sources. It was found that culture media supplemented with 9% SCSP, 3% yeast extract, 1% sodium chloride and 9% glucose augmented protease activity up to 565.80 ± 19.41 U/mL compared to the un-optimized media (170.57 ± 6.75 U/mL). By using this optimized media, the ability and efficiency of B. subtilis in a period of 6 days was investigated whereby acid treated shrimp shells (ATSS) and raw shrimp shell powder (RSSP) were used in substitution of SCSP. In a period of 6 days, the protein content in both ATSS and RSSP was found to have been removed up to 60% and 42% respectively. However deproteinization was found to be more efficient in RSSP with the ratio of tyrosine to protein remained constantly high throughout the 6 days period.
Conclusion, significance and impact of study: A better, more efficient and environmental friendly method is
continuously being improvised to manage shrimp wastes with the use of microbes.
|
| first_indexed | 2025-11-15T09:04:33Z |
| format | Article |
| id | upm-29997 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T09:04:33Z |
| publishDate | 2013 |
| publisher | Malaysian Society for Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-299972015-09-14T01:49:03Z http://psasir.upm.edu.my/id/eprint/29997/ Enhancement of protease production by the optimization of Bacillus subtilis culture medium. Cheong, Jee Yin Mustafa, Muskhazli Abd Aziz, Nor Azwady Go, Rusea Ahmad Adli, Azleen Aims: Traditionally, crustacean wastes have been managed by using acid and alkali which leads to major environmental issue. However, over the recent years microbial fermentation has gained its way whereby producing similar effects as chemical treatment and a higher quality product can be obtained. Extracellular protease from Bacillus subtilis was used further by optimizing its culture medium to enhance protease production. Methodology and Results: The culture media was optimized with 4 various sources; Shrimp Crab Shell Powder (SCSP), nitrogen sources, inorganic salts, and carbon sources. It was found that culture media supplemented with 9% SCSP, 3% yeast extract, 1% sodium chloride and 9% glucose augmented protease activity up to 565.80 ± 19.41 U/mL compared to the un-optimized media (170.57 ± 6.75 U/mL). By using this optimized media, the ability and efficiency of B. subtilis in a period of 6 days was investigated whereby acid treated shrimp shells (ATSS) and raw shrimp shell powder (RSSP) were used in substitution of SCSP. In a period of 6 days, the protein content in both ATSS and RSSP was found to have been removed up to 60% and 42% respectively. However deproteinization was found to be more efficient in RSSP with the ratio of tyrosine to protein remained constantly high throughout the 6 days period. Conclusion, significance and impact of study: A better, more efficient and environmental friendly method is continuously being improvised to manage shrimp wastes with the use of microbes. Malaysian Society for Microbiology 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29997/1/Enhancement%20of%20protease%20production%20by%20the%20optimization%20of%20Bacillus%20subtilis%20culture%20medium.pdf Cheong, Jee Yin and Mustafa, Muskhazli and Abd Aziz, Nor Azwady and Go, Rusea and Ahmad Adli, Azleen (2013) Enhancement of protease production by the optimization of Bacillus subtilis culture medium. Malaysian Journal of Microbiology, 9 (1). pp. 43-50. ISSN 1823-8262; ESSN: 2231-7538 http://web.usm.my/mjm/ English |
| spellingShingle | Cheong, Jee Yin Mustafa, Muskhazli Abd Aziz, Nor Azwady Go, Rusea Ahmad Adli, Azleen Enhancement of protease production by the optimization of Bacillus subtilis culture medium. |
| title | Enhancement of protease production by the optimization of Bacillus subtilis culture medium. |
| title_full | Enhancement of protease production by the optimization of Bacillus subtilis culture medium. |
| title_fullStr | Enhancement of protease production by the optimization of Bacillus subtilis culture medium. |
| title_full_unstemmed | Enhancement of protease production by the optimization of Bacillus subtilis culture medium. |
| title_short | Enhancement of protease production by the optimization of Bacillus subtilis culture medium. |
| title_sort | enhancement of protease production by the optimization of bacillus subtilis culture medium. |
| url | http://psasir.upm.edu.my/id/eprint/29997/ http://psasir.upm.edu.my/id/eprint/29997/ http://psasir.upm.edu.my/id/eprint/29997/1/Enhancement%20of%20protease%20production%20by%20the%20optimization%20of%20Bacillus%20subtilis%20culture%20medium.pdf |