Production of cellulases and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state fermentation
Direct utilization of untreated oil palm trunk (OPT) for cellulases and xylanase production by Aspergillus fumigatus SK1 was conducted under solid-state fermentation (SSF). The highest activities of extracellular cellulases and xylanases were produced at 80% moisture level, initial pH 5.0, 1 × 108 s...
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| Format: | Article |
| Language: | English |
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Elsevier
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28041/ http://psasir.upm.edu.my/id/eprint/28041/1/28041.pdf |
| _version_ | 1848846005460533248 |
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| author | Ang, Siow Kuang Mohamad, Shaza Eva Yahya, Adibah Abd. Aziz, Suraini Md Salleh, Madihah |
| author_facet | Ang, Siow Kuang Mohamad, Shaza Eva Yahya, Adibah Abd. Aziz, Suraini Md Salleh, Madihah |
| author_sort | Ang, Siow Kuang |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Direct utilization of untreated oil palm trunk (OPT) for cellulases and xylanase production by Aspergillus fumigatus SK1 was conducted under solid-state fermentation (SSF). The highest activities of extracellular cellulases and xylanases were produced at 80% moisture level, initial pH 5.0, 1 × 108 spore/g (inoculum) with 125 μm of OPT as sole carbon source. The cellulases and xylanase activities obtained were 54.27, 3.36, 4.54 and 418.70 U/g substrates for endoglucanase (CMCase), exoglucanase (FPase), β-glucosidase and xylanase respectively. The crude cellulases and xylanase required acidic condition to retain their optimum activities (pH 4.0). Crude cellulases and xylanase were more stable at 40°C compared to their optimum activities conditions (60°C for FPase and 70°C for CMCase, β-glucosidase and xylanase). SDS-PAGE and zymogram analysis showed that Aspergillus fumigatus SK1 could secrete cellulases (endoglucanase, exoglucanase and β-glucosidase), xylanase and protease. Enzymatic degradation of alkaline treated OPT with concentrated crude cellulases and xylanases resulted in producing polyoses. |
| first_indexed | 2025-11-15T08:55:50Z |
| format | Article |
| id | upm-28041 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:55:50Z |
| publishDate | 2013 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-280412016-04-25T07:11:23Z http://psasir.upm.edu.my/id/eprint/28041/ Production of cellulases and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state fermentation Ang, Siow Kuang Mohamad, Shaza Eva Yahya, Adibah Abd. Aziz, Suraini Md Salleh, Madihah Direct utilization of untreated oil palm trunk (OPT) for cellulases and xylanase production by Aspergillus fumigatus SK1 was conducted under solid-state fermentation (SSF). The highest activities of extracellular cellulases and xylanases were produced at 80% moisture level, initial pH 5.0, 1 × 108 spore/g (inoculum) with 125 μm of OPT as sole carbon source. The cellulases and xylanase activities obtained were 54.27, 3.36, 4.54 and 418.70 U/g substrates for endoglucanase (CMCase), exoglucanase (FPase), β-glucosidase and xylanase respectively. The crude cellulases and xylanase required acidic condition to retain their optimum activities (pH 4.0). Crude cellulases and xylanase were more stable at 40°C compared to their optimum activities conditions (60°C for FPase and 70°C for CMCase, β-glucosidase and xylanase). SDS-PAGE and zymogram analysis showed that Aspergillus fumigatus SK1 could secrete cellulases (endoglucanase, exoglucanase and β-glucosidase), xylanase and protease. Enzymatic degradation of alkaline treated OPT with concentrated crude cellulases and xylanases resulted in producing polyoses. Elsevier 2013-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28041/1/28041.pdf Ang, Siow Kuang and Mohamad, Shaza Eva and Yahya, Adibah and Abd. Aziz, Suraini and Md Salleh, Madihah (2013) Production of cellulases and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state fermentation. Process Biochemistry, 48 (9). pp. 1293-1302. ISSN 1359-5113; ESSN: 1873-3298 http://www.sciencedirect.com/science/article/pii/S1359511313002894 10.1016/j.procbio.2013.06.019 |
| spellingShingle | Ang, Siow Kuang Mohamad, Shaza Eva Yahya, Adibah Abd. Aziz, Suraini Md Salleh, Madihah Production of cellulases and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state fermentation |
| title | Production of cellulases and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state fermentation |
| title_full | Production of cellulases and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state fermentation |
| title_fullStr | Production of cellulases and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state fermentation |
| title_full_unstemmed | Production of cellulases and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state fermentation |
| title_short | Production of cellulases and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state fermentation |
| title_sort | production of cellulases and xylanase by aspergillus fumigatus sk1 using untreated oil palm trunk through solid state fermentation |
| url | http://psasir.upm.edu.my/id/eprint/28041/ http://psasir.upm.edu.my/id/eprint/28041/ http://psasir.upm.edu.my/id/eprint/28041/ http://psasir.upm.edu.my/id/eprint/28041/1/28041.pdf |