Antioxidant properties of tropical juices and their effects on in vitro hemoglobin and low density lipoprotein (LDL) oxidations

Antioxidant properties of tropical juices, namely bambangan (Mangifera pajang), cocoa (Theobroma cacao) pulp and guava (Psidium guajava) juices and their effects on in vitro hemoglobin and low density lipoprotein (LDL) oxidations were determined. Total phenolics and its compounds in selected jui...

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Main Authors: Ahmad Aufa, Zabidah, Kong, Kin Weng, Ismail, Amin
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24458/
http://psasir.upm.edu.my/id/eprint/24458/1/24458.pdf
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author Ahmad Aufa, Zabidah
Kong, Kin Weng
Ismail, Amin
author_facet Ahmad Aufa, Zabidah
Kong, Kin Weng
Ismail, Amin
author_sort Ahmad Aufa, Zabidah
building UPM Institutional Repository
collection Online Access
description Antioxidant properties of tropical juices, namely bambangan (Mangifera pajang), cocoa (Theobroma cacao) pulp and guava (Psidium guajava) juices and their effects on in vitro hemoglobin and low density lipoprotein (LDL) oxidations were determined. Total phenolics and its compounds in selected juices were determined using Folin-Ciocalteu assay and high performance liquid chromatography (HPLC), respectively. Evaluation of antioxidant properties was done using in vitro assays namely as diphenyl-1-picrylhydrazyl (DPPH) scavenging, β-carotene bleaching (BCB), hemoglobin and LDL oxidation assays. Guava and bambangan juices had the highest and lowest of total phenolic content (TPC), respectively. Nevertheless, bambangan juice showed the greatest scavenging activity on DPPH radical. Furthermore, antioxidant activity (AA) in BCB assay was in the order of cocoa pulp (79%) > bambangan (76%) > guava (47%) juices. Interestingly, the highest inhibition of malondialdehyde (MDA) formation was exhibited by bambangan juice (0.31 µM MDA) in hemoglobin oxidation, while guava juice (0.27 µM MDA) in LDL oxidation systems. The study indicated that bambangan juice may have a potential to be introduced as functional foods product because of its antioxidant properties
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spelling upm-244582015-06-01T04:13:45Z http://psasir.upm.edu.my/id/eprint/24458/ Antioxidant properties of tropical juices and their effects on in vitro hemoglobin and low density lipoprotein (LDL) oxidations Ahmad Aufa, Zabidah Kong, Kin Weng Ismail, Amin Antioxidant properties of tropical juices, namely bambangan (Mangifera pajang), cocoa (Theobroma cacao) pulp and guava (Psidium guajava) juices and their effects on in vitro hemoglobin and low density lipoprotein (LDL) oxidations were determined. Total phenolics and its compounds in selected juices were determined using Folin-Ciocalteu assay and high performance liquid chromatography (HPLC), respectively. Evaluation of antioxidant properties was done using in vitro assays namely as diphenyl-1-picrylhydrazyl (DPPH) scavenging, β-carotene bleaching (BCB), hemoglobin and LDL oxidation assays. Guava and bambangan juices had the highest and lowest of total phenolic content (TPC), respectively. Nevertheless, bambangan juice showed the greatest scavenging activity on DPPH radical. Furthermore, antioxidant activity (AA) in BCB assay was in the order of cocoa pulp (79%) > bambangan (76%) > guava (47%) juices. Interestingly, the highest inhibition of malondialdehyde (MDA) formation was exhibited by bambangan juice (0.31 µM MDA) in hemoglobin oxidation, while guava juice (0.27 µM MDA) in LDL oxidation systems. The study indicated that bambangan juice may have a potential to be introduced as functional foods product because of its antioxidant properties Faculty of Food Science and Technology, Universiti Putra Malaysia 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24458/1/24458.pdf Ahmad Aufa, Zabidah and Kong, Kin Weng and Ismail, Amin (2011) Antioxidant properties of tropical juices and their effects on in vitro hemoglobin and low density lipoprotein (LDL) oxidations. International Food Research Journal, 18 (2). pp. 549-556. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/volume-18-2011.html
spellingShingle Ahmad Aufa, Zabidah
Kong, Kin Weng
Ismail, Amin
Antioxidant properties of tropical juices and their effects on in vitro hemoglobin and low density lipoprotein (LDL) oxidations
title Antioxidant properties of tropical juices and their effects on in vitro hemoglobin and low density lipoprotein (LDL) oxidations
title_full Antioxidant properties of tropical juices and their effects on in vitro hemoglobin and low density lipoprotein (LDL) oxidations
title_fullStr Antioxidant properties of tropical juices and their effects on in vitro hemoglobin and low density lipoprotein (LDL) oxidations
title_full_unstemmed Antioxidant properties of tropical juices and their effects on in vitro hemoglobin and low density lipoprotein (LDL) oxidations
title_short Antioxidant properties of tropical juices and their effects on in vitro hemoglobin and low density lipoprotein (LDL) oxidations
title_sort antioxidant properties of tropical juices and their effects on in vitro hemoglobin and low density lipoprotein (ldl) oxidations
url http://psasir.upm.edu.my/id/eprint/24458/
http://psasir.upm.edu.my/id/eprint/24458/
http://psasir.upm.edu.my/id/eprint/24458/1/24458.pdf