Regulation of virulence factors by quorum sensing in Vibrio harveyi.
Vibrio harveyi is an important aquatic pathogen that produces several virulence factors. In this study, the effect of quorum sensing, bacterial cell-to-cell communication, on the production of the virulence factors caseinase, gelatinase, lipase, hemolysin, and phospholipase, was investigated. The ac...
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| Format: | Article |
| Language: | English English |
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Elsevier
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23850/ http://psasir.upm.edu.my/id/eprint/23850/1/Regulation%20of%20virulence%20factors%20by%20quorum%20sensing%20in%20Vibrio%20harveyi.pdf |
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| author | Mohd Ikhsan, Natrah Fatin Ruwandeepika, H.A. Darshanee Pawar, Sushant Karunasagar, Indrani Sorgeloos, Patrick Bossier, Peter Defoirdt, Tom |
| author_facet | Mohd Ikhsan, Natrah Fatin Ruwandeepika, H.A. Darshanee Pawar, Sushant Karunasagar, Indrani Sorgeloos, Patrick Bossier, Peter Defoirdt, Tom |
| author_sort | Mohd Ikhsan, Natrah Fatin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Vibrio harveyi is an important aquatic pathogen that produces several virulence factors. In this study, the effect of quorum sensing, bacterial cell-to-cell communication, on the production of the virulence factors caseinase, gelatinase, lipase, hemolysin, and phospholipase, was investigated. The activity of virulence factors was studied through enzymatic plate assays using V. harveyi wild type and mutants with constitutively maximal or minimal quorum sensing activity. The results showed that quorum sensing negatively regulates phospholipase activity as higher activity was observed in mutants with minimal quorum sensing activity than in the mutant with maximal quorum sensing activity. Reverse transcriptase real-time PCR with specific primers revealed that the expression level of three phospholipase genes was 2-fold lower in the mutant with minimal quorum sensing activity than in the mutant with maximal quorum sensing activity. As far as we know, this is the first report of quorum sensing regulation of phospholipase. Finally, caseinase and gelatinase activity were positively regulated by quorum sensing, which is consistent with previous reports, and lipase and hemolysin activity were found to be independent of quorum sensing. Hence, the regulation is different for different virulence factors, with some being either positively or negatively regulated, and others being independent of quorum sensing. This might reflect the need to produce the different virulence factors at different stages during infection. |
| first_indexed | 2025-11-15T08:37:50Z |
| format | Article |
| id | upm-23850 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:37:50Z |
| publishDate | 2011 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-238502015-10-09T08:57:43Z http://psasir.upm.edu.my/id/eprint/23850/ Regulation of virulence factors by quorum sensing in Vibrio harveyi. Mohd Ikhsan, Natrah Fatin Ruwandeepika, H.A. Darshanee Pawar, Sushant Karunasagar, Indrani Sorgeloos, Patrick Bossier, Peter Defoirdt, Tom Vibrio harveyi is an important aquatic pathogen that produces several virulence factors. In this study, the effect of quorum sensing, bacterial cell-to-cell communication, on the production of the virulence factors caseinase, gelatinase, lipase, hemolysin, and phospholipase, was investigated. The activity of virulence factors was studied through enzymatic plate assays using V. harveyi wild type and mutants with constitutively maximal or minimal quorum sensing activity. The results showed that quorum sensing negatively regulates phospholipase activity as higher activity was observed in mutants with minimal quorum sensing activity than in the mutant with maximal quorum sensing activity. Reverse transcriptase real-time PCR with specific primers revealed that the expression level of three phospholipase genes was 2-fold lower in the mutant with minimal quorum sensing activity than in the mutant with maximal quorum sensing activity. As far as we know, this is the first report of quorum sensing regulation of phospholipase. Finally, caseinase and gelatinase activity were positively regulated by quorum sensing, which is consistent with previous reports, and lipase and hemolysin activity were found to be independent of quorum sensing. Hence, the regulation is different for different virulence factors, with some being either positively or negatively regulated, and others being independent of quorum sensing. This might reflect the need to produce the different virulence factors at different stages during infection. Elsevier 2011-12-29 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23850/1/Regulation%20of%20virulence%20factors%20by%20quorum%20sensing%20in%20Vibrio%20harveyi.pdf Mohd Ikhsan, Natrah Fatin and Ruwandeepika, H.A. Darshanee and Pawar, Sushant and Karunasagar, Indrani and Sorgeloos, Patrick and Bossier, Peter and Defoirdt, Tom (2011) Regulation of virulence factors by quorum sensing in Vibrio harveyi. Veterinary Microbiology, 154 (1). pp. 124-129. ISSN 0378-1135; ESSN:1873-2542 http://www.elsevier.com 10.1016/j.vetmic.2011.06.024 English |
| spellingShingle | Mohd Ikhsan, Natrah Fatin Ruwandeepika, H.A. Darshanee Pawar, Sushant Karunasagar, Indrani Sorgeloos, Patrick Bossier, Peter Defoirdt, Tom Regulation of virulence factors by quorum sensing in Vibrio harveyi. |
| title | Regulation of virulence factors by quorum sensing in Vibrio harveyi. |
| title_full | Regulation of virulence factors by quorum sensing in Vibrio harveyi. |
| title_fullStr | Regulation of virulence factors by quorum sensing in Vibrio harveyi. |
| title_full_unstemmed | Regulation of virulence factors by quorum sensing in Vibrio harveyi. |
| title_short | Regulation of virulence factors by quorum sensing in Vibrio harveyi. |
| title_sort | regulation of virulence factors by quorum sensing in vibrio harveyi. |
| url | http://psasir.upm.edu.my/id/eprint/23850/ http://psasir.upm.edu.my/id/eprint/23850/ http://psasir.upm.edu.my/id/eprint/23850/ http://psasir.upm.edu.my/id/eprint/23850/1/Regulation%20of%20virulence%20factors%20by%20quorum%20sensing%20in%20Vibrio%20harveyi.pdf |