Development of single-locus DNA microsatellite markers using 50anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia
Horseshoe crabs are said to be declining worldwide. However, there is still no published report on the status of horseshoe crabs in Malaysia. Thus, we report here eight informative microsatellite markers that were developed using the 50-anchored ISSR-PCR enrichment procedure to diagnose the populati...
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| Format: | Article |
| Language: | English |
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Springer Link
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23587/ http://psasir.upm.edu.my/id/eprint/23587/1/Development%20of%20single-locus%20DNA.pdf |
| _version_ | 1848844799813091328 |
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| author | Abu Bakar, Adibah Liew, Pui Ling Tan, Soon Guan Christianus, Annie Qamaruz Zaman, Faridah |
| author_facet | Abu Bakar, Adibah Liew, Pui Ling Tan, Soon Guan Christianus, Annie Qamaruz Zaman, Faridah |
| author_sort | Abu Bakar, Adibah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Horseshoe crabs are said to be declining worldwide. However, there is still no published report on the status of horseshoe crabs in Malaysia. Thus, we report here eight informative microsatellite markers that were developed using the 50-anchored ISSR-PCR enrichment procedure to diagnose the population genetic structure of the mangrove horseshoe crab, Carcinoscorpius rotundicauda from Peninsular Malaysia. This set of markers was tested on 127 samples and showed polymorphism in this species. Hence they should be useful in future essential population genetic studies of these living fossils in the Southeast Asian region. |
| first_indexed | 2025-11-15T08:36:40Z |
| format | Article |
| id | upm-23587 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:36:40Z |
| publishDate | 2012 |
| publisher | Springer Link |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-235872016-02-05T04:31:44Z http://psasir.upm.edu.my/id/eprint/23587/ Development of single-locus DNA microsatellite markers using 50anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia Abu Bakar, Adibah Liew, Pui Ling Tan, Soon Guan Christianus, Annie Qamaruz Zaman, Faridah Horseshoe crabs are said to be declining worldwide. However, there is still no published report on the status of horseshoe crabs in Malaysia. Thus, we report here eight informative microsatellite markers that were developed using the 50-anchored ISSR-PCR enrichment procedure to diagnose the population genetic structure of the mangrove horseshoe crab, Carcinoscorpius rotundicauda from Peninsular Malaysia. This set of markers was tested on 127 samples and showed polymorphism in this species. Hence they should be useful in future essential population genetic studies of these living fossils in the Southeast Asian region. Springer Link 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23587/1/Development%20of%20single-locus%20DNA.pdf Abu Bakar, Adibah and Liew, Pui Ling and Tan, Soon Guan and Christianus, Annie and Qamaruz Zaman, Faridah (2012) Development of single-locus DNA microsatellite markers using 50anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia. Molecular Biology Reports, 39 (3). pp. 3815-3820. ISSN 0301-4851; ESSN:1573-4978 http://dx.doi.org/10.1007/s11033-011-1159-6 10.1007/s11033-011-1159-6 |
| spellingShingle | Abu Bakar, Adibah Liew, Pui Ling Tan, Soon Guan Christianus, Annie Qamaruz Zaman, Faridah Development of single-locus DNA microsatellite markers using 50anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia |
| title | Development of single-locus DNA microsatellite markers using 50anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia |
| title_full | Development of single-locus DNA microsatellite markers using 50anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia |
| title_fullStr | Development of single-locus DNA microsatellite markers using 50anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia |
| title_full_unstemmed | Development of single-locus DNA microsatellite markers using 50anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia |
| title_short | Development of single-locus DNA microsatellite markers using 50anchored ISSR-PCR method for the mangrove horseshoe crab, Carcinoscorpius rotundicauda (Latreille, 1802) in Peninsular Malaysia |
| title_sort | development of single-locus dna microsatellite markers using 50anchored issr-pcr method for the mangrove horseshoe crab, carcinoscorpius rotundicauda (latreille, 1802) in peninsular malaysia |
| url | http://psasir.upm.edu.my/id/eprint/23587/ http://psasir.upm.edu.my/id/eprint/23587/ http://psasir.upm.edu.my/id/eprint/23587/ http://psasir.upm.edu.my/id/eprint/23587/1/Development%20of%20single-locus%20DNA.pdf |