Hypoglycemic effects of cocoa (Theobroma cacao L.) autolysates

Fat, alkaloid and polyphenol contents of two clones of cocoa (UIT1 and PBC 140) were removed and the remaining powder was autolyzed at pH 3.5 and 5.2. Based on the results, autolysates of UIT produced at pH 3.5 exhibited the highest ability to inhibit α-amylase activity. However, no α-glucosidase in...

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Main Authors: Sarmadi, Bahareh, Aminuddin, Farhana, Hamid, Muhajir, Saari, Nazamid, Abdul Hamid, Azizah, Ismail, Amin
Format: Article
Language:English
Published: Elsevier 2012
Online Access:http://psasir.upm.edu.my/id/eprint/22422/
http://psasir.upm.edu.my/id/eprint/22422/1/Hypoglycemic%20effects%20of%20cocoa.pdf
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author Sarmadi, Bahareh
Aminuddin, Farhana
Hamid, Muhajir
Saari, Nazamid
Abdul Hamid, Azizah
Ismail, Amin
author_facet Sarmadi, Bahareh
Aminuddin, Farhana
Hamid, Muhajir
Saari, Nazamid
Abdul Hamid, Azizah
Ismail, Amin
author_sort Sarmadi, Bahareh
building UPM Institutional Repository
collection Online Access
description Fat, alkaloid and polyphenol contents of two clones of cocoa (UIT1 and PBC 140) were removed and the remaining powder was autolyzed at pH 3.5 and 5.2. Based on the results, autolysates of UIT produced at pH 3.5 exhibited the highest ability to inhibit α-amylase activity. However, no α-glucosidase inhibition activity was observed under the conditions specified. Autolysates produced under pH 3.5 caused the highest amount of insulin secretion. In streptozotocin-diabetic rats, all cocoa autolysates significantly decreased blood glucose at 4 h. To assure that the results from the assays were not due to the polyphenols of cocoa autolysates qualitative and quantitative tests were applied. According to their results cocoa autolysates were found to be free from polyphenols. Analysis of amino acid composition revealed that cocoa autolysates were abundant in hydrophobic amino acids. It can be suggested that besides other compounds of cocoa, its peptides and amino acids could contribute to its health benefits.
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publishDate 2012
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spelling upm-224222016-02-17T02:19:32Z http://psasir.upm.edu.my/id/eprint/22422/ Hypoglycemic effects of cocoa (Theobroma cacao L.) autolysates Sarmadi, Bahareh Aminuddin, Farhana Hamid, Muhajir Saari, Nazamid Abdul Hamid, Azizah Ismail, Amin Fat, alkaloid and polyphenol contents of two clones of cocoa (UIT1 and PBC 140) were removed and the remaining powder was autolyzed at pH 3.5 and 5.2. Based on the results, autolysates of UIT produced at pH 3.5 exhibited the highest ability to inhibit α-amylase activity. However, no α-glucosidase inhibition activity was observed under the conditions specified. Autolysates produced under pH 3.5 caused the highest amount of insulin secretion. In streptozotocin-diabetic rats, all cocoa autolysates significantly decreased blood glucose at 4 h. To assure that the results from the assays were not due to the polyphenols of cocoa autolysates qualitative and quantitative tests were applied. According to their results cocoa autolysates were found to be free from polyphenols. Analysis of amino acid composition revealed that cocoa autolysates were abundant in hydrophobic amino acids. It can be suggested that besides other compounds of cocoa, its peptides and amino acids could contribute to its health benefits. Elsevier 2012-09-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22422/1/Hypoglycemic%20effects%20of%20cocoa.pdf Sarmadi, Bahareh and Aminuddin, Farhana and Hamid, Muhajir and Saari, Nazamid and Abdul Hamid, Azizah and Ismail, Amin (2012) Hypoglycemic effects of cocoa (Theobroma cacao L.) autolysates. Food Chemistry, 134 (2). pp. 905-911. ISSN 0308-8146; ESSN: 1873-7072 10.1016/j.foodchem.2012.02.202
spellingShingle Sarmadi, Bahareh
Aminuddin, Farhana
Hamid, Muhajir
Saari, Nazamid
Abdul Hamid, Azizah
Ismail, Amin
Hypoglycemic effects of cocoa (Theobroma cacao L.) autolysates
title Hypoglycemic effects of cocoa (Theobroma cacao L.) autolysates
title_full Hypoglycemic effects of cocoa (Theobroma cacao L.) autolysates
title_fullStr Hypoglycemic effects of cocoa (Theobroma cacao L.) autolysates
title_full_unstemmed Hypoglycemic effects of cocoa (Theobroma cacao L.) autolysates
title_short Hypoglycemic effects of cocoa (Theobroma cacao L.) autolysates
title_sort hypoglycemic effects of cocoa (theobroma cacao l.) autolysates
url http://psasir.upm.edu.my/id/eprint/22422/
http://psasir.upm.edu.my/id/eprint/22422/
http://psasir.upm.edu.my/id/eprint/22422/1/Hypoglycemic%20effects%20of%20cocoa.pdf