Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration
Protocorm-like bodies (PLBs) of Phalaenopsis bellina were successfully cryopreserved by the encapsulation-dehydration approach. Various stages in obtaining successful cryopreservation using this method were optimized. Encapsulated PLBs precultured in half-strength MS medium supplemented with 0.75 M...
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| Format: | Article |
| Language: | English |
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Springer Netherlands
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22370/ http://psasir.upm.edu.my/id/eprint/22370/1/Cryopreservation%20of%20protocorm.pdf |
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| author | Khoddamzadeh, Amir Ali Sinniah, Uma Rani Lynch, Paul Abdul Kadir, Mihdzar Kadzimin, Saleh Mahmood, Maziah |
| author_facet | Khoddamzadeh, Amir Ali Sinniah, Uma Rani Lynch, Paul Abdul Kadir, Mihdzar Kadzimin, Saleh Mahmood, Maziah |
| author_sort | Khoddamzadeh, Amir Ali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Protocorm-like bodies (PLBs) of Phalaenopsis bellina were successfully cryopreserved by the encapsulation-dehydration approach. Various stages in obtaining successful cryopreservation using this method were optimized. Encapsulated PLBs precultured in half-strength MS medium supplemented with 0.75 M sucrose for 3 days exhibited the highest viability in terms of 2,3,5-triphenyltetrazoliumchloride (TTC) reduction. The amount of sucrose in the PLBs after incubation in different concentrations of sucrose for different periods of time determined by HPLC. The highest sucrose concentration was 7 mg/g of PLBs for the PLBs treated with 0.75 M sucrose for 3 days as compared to the control which had only 1 mg/g sucrose. After sucrose preculture, the PLBs were subjected to desiccation using one of two methods. Desiccation using silica gel was more efficient in reducing PLBs moisture content. After 6 h of desiccation, PLBs desiccated using laminar air flow had 43.5% moisture content while for those desiccated using silica gel had 32% moisture content. PLBs desiccated to different moisture contents were plunged into LN. After storage in LN the encapsulated PLBs were re-warmed. Two weeks after re-warming PLBs viability was determined by TTC reduction and re-growth assessed. Encapsulated PLBs precultured with 0.75 M sucrose for 3 days followed by desiccated using silica gel for 5 h resulting in a moisture content of 39% lead to the highest post re-warming viability in terms of TTC reduction (46.6% of control PLBs) and 30% re-growth. |
| first_indexed | 2025-11-15T08:31:23Z |
| format | Article |
| id | upm-22370 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:31:23Z |
| publishDate | 2011 |
| publisher | Springer Netherlands |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-223702015-09-22T00:11:15Z http://psasir.upm.edu.my/id/eprint/22370/ Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration Khoddamzadeh, Amir Ali Sinniah, Uma Rani Lynch, Paul Abdul Kadir, Mihdzar Kadzimin, Saleh Mahmood, Maziah Protocorm-like bodies (PLBs) of Phalaenopsis bellina were successfully cryopreserved by the encapsulation-dehydration approach. Various stages in obtaining successful cryopreservation using this method were optimized. Encapsulated PLBs precultured in half-strength MS medium supplemented with 0.75 M sucrose for 3 days exhibited the highest viability in terms of 2,3,5-triphenyltetrazoliumchloride (TTC) reduction. The amount of sucrose in the PLBs after incubation in different concentrations of sucrose for different periods of time determined by HPLC. The highest sucrose concentration was 7 mg/g of PLBs for the PLBs treated with 0.75 M sucrose for 3 days as compared to the control which had only 1 mg/g sucrose. After sucrose preculture, the PLBs were subjected to desiccation using one of two methods. Desiccation using silica gel was more efficient in reducing PLBs moisture content. After 6 h of desiccation, PLBs desiccated using laminar air flow had 43.5% moisture content while for those desiccated using silica gel had 32% moisture content. PLBs desiccated to different moisture contents were plunged into LN. After storage in LN the encapsulated PLBs were re-warmed. Two weeks after re-warming PLBs viability was determined by TTC reduction and re-growth assessed. Encapsulated PLBs precultured with 0.75 M sucrose for 3 days followed by desiccated using silica gel for 5 h resulting in a moisture content of 39% lead to the highest post re-warming viability in terms of TTC reduction (46.6% of control PLBs) and 30% re-growth. Springer Netherlands 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22370/1/Cryopreservation%20of%20protocorm.pdf Khoddamzadeh, Amir Ali and Sinniah, Uma Rani and Lynch, Paul and Abdul Kadir, Mihdzar and Kadzimin, Saleh and Mahmood, Maziah (2011) Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration. Plant Cell, Tissue and Organ Culture , 107 (3). pp. 471-481. ISSN 0167-6857; ESSN: 1573-5044 10.1007/s11240-011-9997-4 |
| spellingShingle | Khoddamzadeh, Amir Ali Sinniah, Uma Rani Lynch, Paul Abdul Kadir, Mihdzar Kadzimin, Saleh Mahmood, Maziah Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration |
| title | Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration |
| title_full | Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration |
| title_fullStr | Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration |
| title_full_unstemmed | Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration |
| title_short | Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration |
| title_sort | cryopreservation of protocorm-like bodies (plbs) of phalaenopsis bellina (rchb.f.) christenson by encapsulation-dehydration |
| url | http://psasir.upm.edu.my/id/eprint/22370/ http://psasir.upm.edu.my/id/eprint/22370/ http://psasir.upm.edu.my/id/eprint/22370/1/Cryopreservation%20of%20protocorm.pdf |