Conformational destabilization of Bacillus licheniformis α-amylase induced by lysine modification and calcium depletion

Conformational destabilization of Bacillus licheniformis α-amylase induced by lysine modification and calcium depletion

Bacillus licheniformis α-amylase (BLA) was chemically modified using 100-fold molar excess of succinic anhydride over protein or 0.66 M potassium cyanate to obtain 42 % succinylated and 81 % carbamylated BLAs. Size and charge homogeneity of modified preparations was established by Sephacryl S-200 HR...

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Bibliographic Details
Main Authors: Tan, Cheau Yuaan, Raja Abdul Rahman, Raja Noor Zaliha, Abdul Kadir, Habsah, Tayyab, Saad
Format: Article
Language:English
Published: Polish Biochemical Society 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22322/
http://psasir.upm.edu.my/id/eprint/22322/1/Conformational%20destabilization%20of%20Bacillus%20licheniformis%20%CE%B1.pdf

Internet

http://psasir.upm.edu.my/id/eprint/22322/
http://psasir.upm.edu.my/id/eprint/22322/1/Conformational%20destabilization%20of%20Bacillus%20licheniformis%20%CE%B1.pdf

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