Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica
Morinda elliptica Ridley (Rubiaceae) has been used traditionally as a medicine to treat various diseases in Malaysia and Southeast Asia. In the present study we investigated the immunomodulatory effects of damnacanthal isolated from the roots of Morinda elliptica. The immunomodulatory effect of this...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Informa Healthcare
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/17710/ http://psasir.upm.edu.my/id/eprint/17710/1/Immunomodulatory%20effects%20of%20damnacanthal%20isolated%20from%20roots%20of%20Morinda%20elliptica.pdf |
| _version_ | 1848843317805056000 |
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| author | Mohammed Alitheen, Noorjahan Banu Ali, Abdul Manaf Yeap, Swee Keong Mustafa, Shuhaimi L., Nordin Abd Rahman, Mashitoh |
| author_facet | Mohammed Alitheen, Noorjahan Banu Ali, Abdul Manaf Yeap, Swee Keong Mustafa, Shuhaimi L., Nordin Abd Rahman, Mashitoh |
| author_sort | Mohammed Alitheen, Noorjahan Banu |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Morinda elliptica Ridley (Rubiaceae) has been used traditionally as a medicine to treat various diseases in Malaysia and Southeast Asia. In the present study we investigated the immunomodulatory effects of damnacanthal isolated from the roots of Morinda elliptica. The immunomodulatory effect of this compound was evaluated by using the lymphocyte proliferation assay with mouse thymocytes and human peripheral blood mononuclear cells (PBMC). In addition, the effect of the compound on PBMC cell cycle progression was studied by using flow cytometry. The production of human interleukin-2 and human inteleukin-12 cytokines was also assessed using the enzyme linked immunosorbent assay (ELISA) technique. The lymphocyte proliferation assay showed that damnacanthal was able to activate mouse thymocytes and PBMC at a low concentration (0.468 μg/mL). Moreover, the production of human interleukin-2 and human interleukin-12 cytokines in the culture supernatant from damnacanthal activated lymphocytes was markedly up-regulated at 24 h and sustained until 72 h with a slight decrease with time. A positive correlation was found between the level of these two cytokines and the MTT-based proliferation assay. Based on the above results, damnacanthal can act as an immunomodulatory agent which may be very useful for maintaining a healthy immune system. |
| first_indexed | 2025-11-15T08:13:07Z |
| format | Article |
| id | upm-17710 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:13:07Z |
| publishDate | 2010 |
| publisher | Informa Healthcare |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-177102017-09-28T09:46:20Z http://psasir.upm.edu.my/id/eprint/17710/ Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica Mohammed Alitheen, Noorjahan Banu Ali, Abdul Manaf Yeap, Swee Keong Mustafa, Shuhaimi L., Nordin Abd Rahman, Mashitoh Morinda elliptica Ridley (Rubiaceae) has been used traditionally as a medicine to treat various diseases in Malaysia and Southeast Asia. In the present study we investigated the immunomodulatory effects of damnacanthal isolated from the roots of Morinda elliptica. The immunomodulatory effect of this compound was evaluated by using the lymphocyte proliferation assay with mouse thymocytes and human peripheral blood mononuclear cells (PBMC). In addition, the effect of the compound on PBMC cell cycle progression was studied by using flow cytometry. The production of human interleukin-2 and human inteleukin-12 cytokines was also assessed using the enzyme linked immunosorbent assay (ELISA) technique. The lymphocyte proliferation assay showed that damnacanthal was able to activate mouse thymocytes and PBMC at a low concentration (0.468 μg/mL). Moreover, the production of human interleukin-2 and human interleukin-12 cytokines in the culture supernatant from damnacanthal activated lymphocytes was markedly up-regulated at 24 h and sustained until 72 h with a slight decrease with time. A positive correlation was found between the level of these two cytokines and the MTT-based proliferation assay. Based on the above results, damnacanthal can act as an immunomodulatory agent which may be very useful for maintaining a healthy immune system. Informa Healthcare 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17710/1/Immunomodulatory%20effects%20of%20damnacanthal%20isolated%20from%20roots%20of%20Morinda%20elliptica.pdf Mohammed Alitheen, Noorjahan Banu and Ali, Abdul Manaf and Yeap, Swee Keong and Mustafa, Shuhaimi and L., Nordin and Abd Rahman, Mashitoh (2010) Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica. Pharmaceutical Biology, 48 (4). pp. 446-452. ISSN 1388-0209; ESSN: 1744-5116 10.3109/13880200903168031 |
| spellingShingle | Mohammed Alitheen, Noorjahan Banu Ali, Abdul Manaf Yeap, Swee Keong Mustafa, Shuhaimi L., Nordin Abd Rahman, Mashitoh Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica |
| title | Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica |
| title_full | Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica |
| title_fullStr | Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica |
| title_full_unstemmed | Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica |
| title_short | Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica |
| title_sort | immunomodulatory effects of damnacanthal isolated from roots of morinda elliptica |
| url | http://psasir.upm.edu.my/id/eprint/17710/ http://psasir.upm.edu.my/id/eprint/17710/ http://psasir.upm.edu.my/id/eprint/17710/1/Immunomodulatory%20effects%20of%20damnacanthal%20isolated%20from%20roots%20of%20Morinda%20elliptica.pdf |