Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5...
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16422/ http://psasir.upm.edu.my/id/eprint/16422/1/16422.pdf |
| _version_ | 1848842958279802880 |
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| author | Yusop, Zulkifli Mohamed Alitheen, Noorjahan Banu Radu, Son Abdul Rahim, Raha Lihan, Samuel Yeap, Swee Keong Nishibuchi, Mitsuaki |
| author_facet | Yusop, Zulkifli Mohamed Alitheen, Noorjahan Banu Radu, Son Abdul Rahim, Raha Lihan, Samuel Yeap, Swee Keong Nishibuchi, Mitsuaki |
| author_sort | Yusop, Zulkifli |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5 kb. Both primers produced twenty five RAPD patterns with a few isolates failed to produce any products. Based
on phylogenetic dendrogram, all the isolates can be divided into 6 major clusters with similarity between 0 to 52%. For the ERIC primer, it produced bands ranged from 3-15 with sizes from 0.1 – 5.0 kb and twenty seven different ERIC patterns. Construction of the phylogenetic dendogram showed the isolates can be divided into 4 major clusters with similarity between 56 to 86%. The high diversity of both processes may be due to the multiple contamination sources of V. parahaemolyticus. |
| first_indexed | 2025-11-15T08:07:24Z |
| format | Article |
| id | upm-16422 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:07:24Z |
| publishDate | 2009 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-164222015-09-25T00:45:53Z http://psasir.upm.edu.my/id/eprint/16422/ Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia Yusop, Zulkifli Mohamed Alitheen, Noorjahan Banu Radu, Son Abdul Rahim, Raha Lihan, Samuel Yeap, Swee Keong Nishibuchi, Mitsuaki In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5 kb. Both primers produced twenty five RAPD patterns with a few isolates failed to produce any products. Based on phylogenetic dendrogram, all the isolates can be divided into 6 major clusters with similarity between 0 to 52%. For the ERIC primer, it produced bands ranged from 3-15 with sizes from 0.1 – 5.0 kb and twenty seven different ERIC patterns. Construction of the phylogenetic dendogram showed the isolates can be divided into 4 major clusters with similarity between 56 to 86%. The high diversity of both processes may be due to the multiple contamination sources of V. parahaemolyticus. Faculty of Food Science and Technology, Universiti Putra Malaysia 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16422/1/16422.pdf Yusop, Zulkifli and Mohamed Alitheen, Noorjahan Banu and Radu, Son and Abdul Rahim, Raha and Lihan, Samuel and Yeap, Swee Keong and Nishibuchi, Mitsuaki (2009) Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia. International Food Research Journal, 16 (2). pp. 141-150. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/16%20%282%29%202009/03-%20IFRJ-2008-125%20Noorjahan%20Malaysia%202nd%20proof.pdf |
| spellingShingle | Yusop, Zulkifli Mohamed Alitheen, Noorjahan Banu Radu, Son Abdul Rahim, Raha Lihan, Samuel Yeap, Swee Keong Nishibuchi, Mitsuaki Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
| title | Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
| title_full | Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
| title_fullStr | Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
| title_full_unstemmed | Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
| title_short | Random amplified polymorphic DNA-PCR and ERIC PCR analysis on
Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia |
| title_sort | random amplified polymorphic dna-pcr and eric pcr analysis on
vibrio parahaemolyticus isolated from cockles in padang, indonesia |
| url | http://psasir.upm.edu.my/id/eprint/16422/ http://psasir.upm.edu.my/id/eprint/16422/ http://psasir.upm.edu.my/id/eprint/16422/1/16422.pdf |