Validation of a HPLC/FLD method for quantification of tocotrienols in human plasma
Quantification of tocotrienols in human plasma is critical when the attention towards tocotrienols on its distinctive properties is arising. We aim to develop a simple and practical normal-phase high performance liquid chromatography method to quantify the amount of four tocotrienol homologues in hu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishing Corporation
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15893/ http://psasir.upm.edu.my/id/eprint/15893/1/357609.pdf |
| _version_ | 1848842807077240832 |
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| author | Che, Hui Ling Tan, Doryn Meam Yee Meganathan, Puvaneswari Gan, Yee Lin Abdul Razak, Ghazali Fu, Ju Yen |
| author_facet | Che, Hui Ling Tan, Doryn Meam Yee Meganathan, Puvaneswari Gan, Yee Lin Abdul Razak, Ghazali Fu, Ju Yen |
| author_sort | Che, Hui Ling |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Quantification of tocotrienols in human plasma is critical when the attention towards tocotrienols on its distinctive properties is arising. We aim to develop a simple and practical normal-phase high performance liquid chromatography method to quantify the amount of four tocotrienol homologues in human plasma. Using both the external and internal standards, tocotrienol homologues were quantified via a normal-phase high performance liquid chromatography with fluorescence detector maintained at the excitation wavelength of 295 nm and the emission wavelength of 325 nm. The four tocotrienol homologues were well separated within 30 minutes. A large interindividual variation between subjects was observed as the absorption of tocotrienols is dependent on food matrix and gut lipolysis. The accuracies of lower and upper limit of quantification ranged between 92% and 109% for intraday assays and 90% and 112% for interday assays. This method was successfully applied to quantify the total amount of four tocotrienol homologues in human plasma. |
| first_indexed | 2025-11-15T08:05:00Z |
| format | Article |
| id | upm-15893 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:05:00Z |
| publishDate | 2015 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-158932019-11-12T02:54:22Z http://psasir.upm.edu.my/id/eprint/15893/ Validation of a HPLC/FLD method for quantification of tocotrienols in human plasma Che, Hui Ling Tan, Doryn Meam Yee Meganathan, Puvaneswari Gan, Yee Lin Abdul Razak, Ghazali Fu, Ju Yen Quantification of tocotrienols in human plasma is critical when the attention towards tocotrienols on its distinctive properties is arising. We aim to develop a simple and practical normal-phase high performance liquid chromatography method to quantify the amount of four tocotrienol homologues in human plasma. Using both the external and internal standards, tocotrienol homologues were quantified via a normal-phase high performance liquid chromatography with fluorescence detector maintained at the excitation wavelength of 295 nm and the emission wavelength of 325 nm. The four tocotrienol homologues were well separated within 30 minutes. A large interindividual variation between subjects was observed as the absorption of tocotrienols is dependent on food matrix and gut lipolysis. The accuracies of lower and upper limit of quantification ranged between 92% and 109% for intraday assays and 90% and 112% for interday assays. This method was successfully applied to quantify the total amount of four tocotrienol homologues in human plasma. Hindawi Publishing Corporation 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/15893/1/357609.pdf Che, Hui Ling and Tan, Doryn Meam Yee and Meganathan, Puvaneswari and Gan, Yee Lin and Abdul Razak, Ghazali and Fu, Ju Yen (2015) Validation of a HPLC/FLD method for quantification of tocotrienols in human plasma. International Journal of Analytical Chemistry, 2015. art. no. 357609. pp. 1-7. ISSN 1687-8760; ESSN: 1687-8779 https://www.hindawi.com/journals/ijac/2015/357609/ 10.1155/2015/357609 |
| spellingShingle | Che, Hui Ling Tan, Doryn Meam Yee Meganathan, Puvaneswari Gan, Yee Lin Abdul Razak, Ghazali Fu, Ju Yen Validation of a HPLC/FLD method for quantification of tocotrienols in human plasma |
| title | Validation of a HPLC/FLD method for quantification of tocotrienols in human plasma |
| title_full | Validation of a HPLC/FLD method for quantification of tocotrienols in human plasma |
| title_fullStr | Validation of a HPLC/FLD method for quantification of tocotrienols in human plasma |
| title_full_unstemmed | Validation of a HPLC/FLD method for quantification of tocotrienols in human plasma |
| title_short | Validation of a HPLC/FLD method for quantification of tocotrienols in human plasma |
| title_sort | validation of a hplc/fld method for quantification of tocotrienols in human plasma |
| url | http://psasir.upm.edu.my/id/eprint/15893/ http://psasir.upm.edu.my/id/eprint/15893/ http://psasir.upm.edu.my/id/eprint/15893/ http://psasir.upm.edu.my/id/eprint/15893/1/357609.pdf |