Isolation and identification of epiphytic lactic acid bacteria from guinea grass (Panicum maximum)
Problem statement: Bacteria can perform a variety of beneficial functions, for example many lactic acid bacteria are responsible for fermentation of silage in the process of forage conservation. In the making of silage, epiphytic lactic acid bacteria are usually insufficient in numbers to promote ef...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Science Publications
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15330/ http://psasir.upm.edu.my/id/eprint/15330/1/ajavsp.2010.146.150.pdf |
| _version_ | 1848842646916694016 |
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| author | Pasebani, Mahdi Yaakub, Halimatun Sijam, Kamaruzaman Alimon, Abdul Razak |
| author_facet | Pasebani, Mahdi Yaakub, Halimatun Sijam, Kamaruzaman Alimon, Abdul Razak |
| author_sort | Pasebani, Mahdi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Problem statement: Bacteria can perform a variety of beneficial functions, for example many lactic acid bacteria are responsible for fermentation of silage in the process of forage conservation. In the making of silage, epiphytic lactic acid bacteria are usually insufficient in numbers to promote efficient lactate fermentation. This study was conducted to identify the predominant indigenous bacteria, with emphasis on lactic acid bacteria, from Guinea grass (Panicum maximum). Approach: Two different condition of growth using nutrient and MRS agar were prepared for isolation of the bacteria. In total, 18 purified isolates were identified by BIOLOG identification system which comprised of 9 bacterial species. Standard plate count in the both conditions was considered. Results: Three bacterial species based on the first condition of growth were identified which were belonging to Flavimonas oryzihabitans, Enerobacter cloacae, Sphingomonas paucimobilis B. Lactic acid bacteria based on the second condition of growth were belonging to Weissella confusa, Weissella paramesenteroides, Leuconostoc mesenteroides ssp. dextranicum, Lactococcus lactis ssp. hordniae. Result of plate count showed that 8.3×103 CFU lactic acid bacteria are available per gram of fresh guinea grass. Conclusion: Three hetero-fermentative and one homo-fermentative lactic acid bacteria were identified which would be suggested to use as bacterial inoculants because of the insufficient amount of epiphytic lactic acid bacteria and the availability of pathogenic bacteria in the grass. |
| first_indexed | 2025-11-15T08:02:27Z |
| format | Article |
| id | upm-15330 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:02:27Z |
| publishDate | 2010 |
| publisher | Science Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-153302017-11-22T09:15:15Z http://psasir.upm.edu.my/id/eprint/15330/ Isolation and identification of epiphytic lactic acid bacteria from guinea grass (Panicum maximum) Pasebani, Mahdi Yaakub, Halimatun Sijam, Kamaruzaman Alimon, Abdul Razak Problem statement: Bacteria can perform a variety of beneficial functions, for example many lactic acid bacteria are responsible for fermentation of silage in the process of forage conservation. In the making of silage, epiphytic lactic acid bacteria are usually insufficient in numbers to promote efficient lactate fermentation. This study was conducted to identify the predominant indigenous bacteria, with emphasis on lactic acid bacteria, from Guinea grass (Panicum maximum). Approach: Two different condition of growth using nutrient and MRS agar were prepared for isolation of the bacteria. In total, 18 purified isolates were identified by BIOLOG identification system which comprised of 9 bacterial species. Standard plate count in the both conditions was considered. Results: Three bacterial species based on the first condition of growth were identified which were belonging to Flavimonas oryzihabitans, Enerobacter cloacae, Sphingomonas paucimobilis B. Lactic acid bacteria based on the second condition of growth were belonging to Weissella confusa, Weissella paramesenteroides, Leuconostoc mesenteroides ssp. dextranicum, Lactococcus lactis ssp. hordniae. Result of plate count showed that 8.3×103 CFU lactic acid bacteria are available per gram of fresh guinea grass. Conclusion: Three hetero-fermentative and one homo-fermentative lactic acid bacteria were identified which would be suggested to use as bacterial inoculants because of the insufficient amount of epiphytic lactic acid bacteria and the availability of pathogenic bacteria in the grass. Science Publications 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15330/1/ajavsp.2010.146.150.pdf Pasebani, Mahdi and Yaakub, Halimatun and Sijam, Kamaruzaman and Alimon, Abdul Razak (2010) Isolation and identification of epiphytic lactic acid bacteria from guinea grass (Panicum maximum). American Journal of Animal and Veterinary Sciences, 5 (2). pp. 146-150. ISSN 1557-4555; ESSN: 1557-4563 http://thescipub.com/html/10.3844/ajavsp.2010.146.150 10.3844/ajavsp.2010.146.150 |
| spellingShingle | Pasebani, Mahdi Yaakub, Halimatun Sijam, Kamaruzaman Alimon, Abdul Razak Isolation and identification of epiphytic lactic acid bacteria from guinea grass (Panicum maximum) |
| title | Isolation and identification of epiphytic lactic acid bacteria from guinea grass (Panicum maximum) |
| title_full | Isolation and identification of epiphytic lactic acid bacteria from guinea grass (Panicum maximum) |
| title_fullStr | Isolation and identification of epiphytic lactic acid bacteria from guinea grass (Panicum maximum) |
| title_full_unstemmed | Isolation and identification of epiphytic lactic acid bacteria from guinea grass (Panicum maximum) |
| title_short | Isolation and identification of epiphytic lactic acid bacteria from guinea grass (Panicum maximum) |
| title_sort | isolation and identification of epiphytic lactic acid bacteria from guinea grass (panicum maximum) |
| url | http://psasir.upm.edu.my/id/eprint/15330/ http://psasir.upm.edu.my/id/eprint/15330/ http://psasir.upm.edu.my/id/eprint/15330/ http://psasir.upm.edu.my/id/eprint/15330/1/ajavsp.2010.146.150.pdf |