In vitro ultramorphological assessment of apoptosis on CEMss induce by linoleic acid-rich fraction form Typhonium flagelliforme tuber.
The plant Typhonium flagelliforme, commonly known as "rodent tuber" in Malaysia, is often used as a health supplement and traditional remedy for alternative cancer therapies, including leukemia. This study aimed to evaluate in vitro anti-leukemic activity of dichloromethane extract/fractio...
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| Format: | Article |
| Language: | English English |
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Hindawi Publishing Corporation
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15171/ http://psasir.upm.edu.my/id/eprint/15171/1/In%20vitro%20ultramorphological%20assessment%20of%20apoptosis%20on%20CEMss%20induce%20by%20linoleic%20acid.pdf |
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| author | Mohan, Syam Murali Abdul, Ahmad Bustamam Ibrahim, Siddig Al-Zubairi, Adel Sharaf Md. Sukari, Mohd Aspollah Abdullah, Rasedee Elhassan Taha, Manal Mohamed |
| author_facet | Mohan, Syam Murali Abdul, Ahmad Bustamam Ibrahim, Siddig Al-Zubairi, Adel Sharaf Md. Sukari, Mohd Aspollah Abdullah, Rasedee Elhassan Taha, Manal Mohamed |
| author_sort | Mohan, Syam Murali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The plant Typhonium flagelliforme, commonly known as "rodent tuber" in Malaysia, is often used as a health supplement and traditional remedy for alternative cancer therapies, including leukemia. This study aimed to evaluate in vitro anti-leukemic activity of dichloromethane extract/fraction number 7 (DCM/F7) from T. flagelliforme tuber on human T4 lymphoblastoid (CEMss) cell line. The DCM extract of tuber has been fractionated by column chromatography. The obtained fractions were evaluated for its cytotoxicity toward CEMss cells as well as human primary blood lymphocytes (PBLs). Assessment of apoptosis produced by the most active fraction was evaluated by various microscopic techniques and further confirmation of apoptosis was done by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Phytochemical screening was done by gas chromatography-mass spectrometry (GC-MS). The results shows that 7 out of 12 fractions showed significant cytotoxicity against the selected cell line CEMss, in which fractions DCM/F7, DCM/F11 and DCM/F12 showed exceptional activity with 3, 5 and 6.2 μg ml(-1), respectively. Further studies in the non-cancerous PBL exhibited significant selectivity of DCM/F7 compared to other fractions. Cytological observations showed chromatin condensation, cell shrinkage, abnormalities of cristae, membrane blebbing, cytoplasmic extrusions and formation of apoptotic bodies as confirmed collectively by double-staining of acridine orange (AO)/propidium iodide (PI), SEM and TEM. In addition, DCM/F7 has increased the cellular DNA breaks on treated cells. GC-MS revealed that DCM/F7 contains linoleic acid, hexadecanoic acid and 9-hexadecanoic acid. The present results indicate that T. flagelliforme possess a valuable anti-leukemic effect and was able to produce distinctive morphological features of cell death that corresponds to apoptosis. |
| first_indexed | 2025-11-15T08:01:44Z |
| format | Article |
| id | upm-15171 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:01:44Z |
| publishDate | 2010 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-151712015-10-21T03:37:45Z http://psasir.upm.edu.my/id/eprint/15171/ In vitro ultramorphological assessment of apoptosis on CEMss induce by linoleic acid-rich fraction form Typhonium flagelliforme tuber. Mohan, Syam Murali Abdul, Ahmad Bustamam Ibrahim, Siddig Al-Zubairi, Adel Sharaf Md. Sukari, Mohd Aspollah Abdullah, Rasedee Elhassan Taha, Manal Mohamed The plant Typhonium flagelliforme, commonly known as "rodent tuber" in Malaysia, is often used as a health supplement and traditional remedy for alternative cancer therapies, including leukemia. This study aimed to evaluate in vitro anti-leukemic activity of dichloromethane extract/fraction number 7 (DCM/F7) from T. flagelliforme tuber on human T4 lymphoblastoid (CEMss) cell line. The DCM extract of tuber has been fractionated by column chromatography. The obtained fractions were evaluated for its cytotoxicity toward CEMss cells as well as human primary blood lymphocytes (PBLs). Assessment of apoptosis produced by the most active fraction was evaluated by various microscopic techniques and further confirmation of apoptosis was done by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Phytochemical screening was done by gas chromatography-mass spectrometry (GC-MS). The results shows that 7 out of 12 fractions showed significant cytotoxicity against the selected cell line CEMss, in which fractions DCM/F7, DCM/F11 and DCM/F12 showed exceptional activity with 3, 5 and 6.2 μg ml(-1), respectively. Further studies in the non-cancerous PBL exhibited significant selectivity of DCM/F7 compared to other fractions. Cytological observations showed chromatin condensation, cell shrinkage, abnormalities of cristae, membrane blebbing, cytoplasmic extrusions and formation of apoptotic bodies as confirmed collectively by double-staining of acridine orange (AO)/propidium iodide (PI), SEM and TEM. In addition, DCM/F7 has increased the cellular DNA breaks on treated cells. GC-MS revealed that DCM/F7 contains linoleic acid, hexadecanoic acid and 9-hexadecanoic acid. The present results indicate that T. flagelliforme possess a valuable anti-leukemic effect and was able to produce distinctive morphological features of cell death that corresponds to apoptosis. Hindawi Publishing Corporation 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15171/1/In%20vitro%20ultramorphological%20assessment%20of%20apoptosis%20on%20CEMss%20induce%20by%20linoleic%20acid.pdf Mohan, Syam Murali and Abdul, Ahmad Bustamam and Ibrahim, Siddig and Al-Zubairi, Adel Sharaf and Md. Sukari, Mohd Aspollah and Abdullah, Rasedee and Elhassan Taha, Manal Mohamed (2010) In vitro ultramorphological assessment of apoptosis on CEMss induce by linoleic acid-rich fraction form Typhonium flagelliforme tuber. Evidence-Based Complementary and Alternative Medicine, 2011 (421894). pp. 1-13. ISSN 1741-427X; ESSN: 1741-4288 10.1093/ecam/neq010 English |
| spellingShingle | Mohan, Syam Murali Abdul, Ahmad Bustamam Ibrahim, Siddig Al-Zubairi, Adel Sharaf Md. Sukari, Mohd Aspollah Abdullah, Rasedee Elhassan Taha, Manal Mohamed In vitro ultramorphological assessment of apoptosis on CEMss induce by linoleic acid-rich fraction form Typhonium flagelliforme tuber. |
| title | In vitro ultramorphological assessment of apoptosis on CEMss induce by linoleic acid-rich fraction form Typhonium flagelliforme tuber. |
| title_full | In vitro ultramorphological assessment of apoptosis on CEMss induce by linoleic acid-rich fraction form Typhonium flagelliforme tuber. |
| title_fullStr | In vitro ultramorphological assessment of apoptosis on CEMss induce by linoleic acid-rich fraction form Typhonium flagelliforme tuber. |
| title_full_unstemmed | In vitro ultramorphological assessment of apoptosis on CEMss induce by linoleic acid-rich fraction form Typhonium flagelliforme tuber. |
| title_short | In vitro ultramorphological assessment of apoptosis on CEMss induce by linoleic acid-rich fraction form Typhonium flagelliforme tuber. |
| title_sort | in vitro ultramorphological assessment of apoptosis on cemss induce by linoleic acid-rich fraction form typhonium flagelliforme tuber. |
| url | http://psasir.upm.edu.my/id/eprint/15171/ http://psasir.upm.edu.my/id/eprint/15171/ http://psasir.upm.edu.my/id/eprint/15171/1/In%20vitro%20ultramorphological%20assessment%20of%20apoptosis%20on%20CEMss%20induce%20by%20linoleic%20acid.pdf |