Estimation of 16S RNA gene copy number in several probiotic Lactobacillus strains isolated from the gastrointestinal tract of chicken.
The copy numbers of 16S rRNA genes in 12 probiotic Lactobacillus strains of poultry origin were analyzed. Genomic DNA of the strains was digested with restriction endonucleases that do not cut within the 16S rRNA gene of the strains. This was followed by Southern hybridization with a biotinylated pr...
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| Format: | Article |
| Language: | English English |
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Blackwell Publishing Ltd
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14568/ http://psasir.upm.edu.my/id/eprint/14568/1/Estimation%20of%2016S%20RNA%20gene%20copy%20number%20in%20several%20probiotic%20Lactobacillus%20strains%20isolated%20from%20the%20gastrointestinal%20tract%20of%20chicken.pdf |
| _version_ | 1848842429978902528 |
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| author | Chin, Mei Lee Chin, Chin Sieo Abdullah, Norhani Yin, Wan Ho |
| author_facet | Chin, Mei Lee Chin, Chin Sieo Abdullah, Norhani Yin, Wan Ho |
| author_sort | Chin, Mei Lee |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The copy numbers of 16S rRNA genes in 12 probiotic Lactobacillus strains of poultry origin were analyzed. Genomic DNA of the strains was digested with restriction endonucleases that do not cut within the 16S rRNA gene of the strains. This was followed by Southern hybridization with a biotinylated probe complementary to the 16S rRNA gene. The copy number of the 16S rRNA gene within a Lactobacillus species was found to be conserved. From the hybridization results, Lactobacillus salivarius I 24 was estimated to have seven copies of the 16S rRNA gene, Lactobacillus panis C 17 to have five copies and Lactobacillus gallinarum strains I 16 and I 26 four copies. The 16S rRNA gene copy numbers of L. gallinarum and L. panis reported in the present study are the first record. Lactobacillus brevis strains I 12, I 23, I 25, I 211, I 218 and Lactobacillus reuteri strains C 1, C 10, C 16 were estimated to have at least four copies of the 16S rRNA gene. In addition, distinct rRNA restriction patterns which could discriminate the strains of L. reuteri and L. gallinarum were also detected. Information on 16S rRNA gene copy number is important for physiological, evolutionary and population studies of the bacteria. |
| first_indexed | 2025-11-15T07:59:00Z |
| format | Article |
| id | upm-14568 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:59:00Z |
| publishDate | 2008 |
| publisher | Blackwell Publishing Ltd |
| recordtype | eprints |
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| spelling | upm-145682015-11-23T07:17:28Z http://psasir.upm.edu.my/id/eprint/14568/ Estimation of 16S RNA gene copy number in several probiotic Lactobacillus strains isolated from the gastrointestinal tract of chicken. Chin, Mei Lee Chin, Chin Sieo Abdullah, Norhani Yin, Wan Ho The copy numbers of 16S rRNA genes in 12 probiotic Lactobacillus strains of poultry origin were analyzed. Genomic DNA of the strains was digested with restriction endonucleases that do not cut within the 16S rRNA gene of the strains. This was followed by Southern hybridization with a biotinylated probe complementary to the 16S rRNA gene. The copy number of the 16S rRNA gene within a Lactobacillus species was found to be conserved. From the hybridization results, Lactobacillus salivarius I 24 was estimated to have seven copies of the 16S rRNA gene, Lactobacillus panis C 17 to have five copies and Lactobacillus gallinarum strains I 16 and I 26 four copies. The 16S rRNA gene copy numbers of L. gallinarum and L. panis reported in the present study are the first record. Lactobacillus brevis strains I 12, I 23, I 25, I 211, I 218 and Lactobacillus reuteri strains C 1, C 10, C 16 were estimated to have at least four copies of the 16S rRNA gene. In addition, distinct rRNA restriction patterns which could discriminate the strains of L. reuteri and L. gallinarum were also detected. Information on 16S rRNA gene copy number is important for physiological, evolutionary and population studies of the bacteria. Blackwell Publishing Ltd 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14568/1/Estimation%20of%2016S%20RNA%20gene%20copy%20number%20in%20several%20probiotic%20Lactobacillus%20strains%20isolated%20from%20the%20gastrointestinal%20tract%20of%20chicken.pdf Chin, Mei Lee and Chin, Chin Sieo and Abdullah, Norhani and Yin, Wan Ho (2008) Estimation of 16S RNA gene copy number in several probiotic Lactobacillus strains isolated from the gastrointestinal tract of chicken. FEMS Microbiology Letters, 287 (1). pp. 136-141. ISSN 0378-1097 10.1111/j.1574-6968.2008.01305.x English |
| spellingShingle | Chin, Mei Lee Chin, Chin Sieo Abdullah, Norhani Yin, Wan Ho Estimation of 16S RNA gene copy number in several probiotic Lactobacillus strains isolated from the gastrointestinal tract of chicken. |
| title | Estimation of 16S RNA gene copy number in several probiotic Lactobacillus strains isolated from the gastrointestinal tract of chicken. |
| title_full | Estimation of 16S RNA gene copy number in several probiotic Lactobacillus strains isolated from the gastrointestinal tract of chicken. |
| title_fullStr | Estimation of 16S RNA gene copy number in several probiotic Lactobacillus strains isolated from the gastrointestinal tract of chicken. |
| title_full_unstemmed | Estimation of 16S RNA gene copy number in several probiotic Lactobacillus strains isolated from the gastrointestinal tract of chicken. |
| title_short | Estimation of 16S RNA gene copy number in several probiotic Lactobacillus strains isolated from the gastrointestinal tract of chicken. |
| title_sort | estimation of 16s rna gene copy number in several probiotic lactobacillus strains isolated from the gastrointestinal tract of chicken. |
| url | http://psasir.upm.edu.my/id/eprint/14568/ http://psasir.upm.edu.my/id/eprint/14568/ http://psasir.upm.edu.my/id/eprint/14568/1/Estimation%20of%2016S%20RNA%20gene%20copy%20number%20in%20several%20probiotic%20Lactobacillus%20strains%20isolated%20from%20the%20gastrointestinal%20tract%20of%20chicken.pdf |