Efflux genes and active efflux activity detection in of methicillin-resistant Staphylococcus aureus (MRSA).
Efflux-mediated resistance has been recognized as an important contributor of antibiotic resistance in bacteria, especially in methicillin-resistant Staphylococcus aureus (MRSA) isolates. This study was carried out to detect and analyze efflux genes (norA and mdeA) and active efflux activity in a c...
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| Format: | Article |
| Language: | English |
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Wiley-Blackwell
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/14444/ |
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| author | Saiful, Azmi Johari Mohtar, Mastura Zarizal, Suhaili Mazurah, Mohamed Isa Shuhaimi, Mustafa Ali, Abdul Manaf |
| author_facet | Saiful, Azmi Johari Mohtar, Mastura Zarizal, Suhaili Mazurah, Mohamed Isa Shuhaimi, Mustafa Ali, Abdul Manaf |
| author_sort | Saiful, Azmi Johari |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Efflux-mediated resistance has been recognized as an important contributor of antibiotic resistance in bacteria, especially in methicillin-resistant Staphylococcus aureus (MRSA) isolates. This study was carried out to detect and analyze efflux genes (norA and mdeA) and active efflux
activity in a collection of Malaysian MRSA and methicillin-sensitive S. aureus (MSSA) clinical isolates. Nineteen isolates including three ATCC S. aureus reference strains were subjected to PCR detection and DNA sequence analysis for norA and mdeA and active efflux detection using
modified minimum inhibitory concentration (MIC) assay. From the 19 isolates, 18 isolates harboured the mdeA gene while 16 isolates contained norA gene. DNA sequence analysis reveals 98–100% correlation between the PCR product and the published DNA sequences in GenBank. In addition, 16 isolates exhibited active efflux activity using the ethidium bromide (EtBr)- reserpine combination MIC assay. To our knowledge, this is the first report on the detection of efflux genes and active efflux activity amongst Malaysian clinical isolates of MRSA/MSSA. Detection of active efflux activity may explain the previous report on efflux-mediated drug resistance profile amongst the local clinical isolates. |
| first_indexed | 2025-11-15T07:58:27Z |
| format | Article |
| id | upm-14444 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:58:27Z |
| publishDate | 2008 |
| publisher | Wiley-Blackwell |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-144442012-08-15T07:40:44Z http://psasir.upm.edu.my/id/eprint/14444/ Efflux genes and active efflux activity detection in of methicillin-resistant Staphylococcus aureus (MRSA). Saiful, Azmi Johari Mohtar, Mastura Zarizal, Suhaili Mazurah, Mohamed Isa Shuhaimi, Mustafa Ali, Abdul Manaf Efflux-mediated resistance has been recognized as an important contributor of antibiotic resistance in bacteria, especially in methicillin-resistant Staphylococcus aureus (MRSA) isolates. This study was carried out to detect and analyze efflux genes (norA and mdeA) and active efflux activity in a collection of Malaysian MRSA and methicillin-sensitive S. aureus (MSSA) clinical isolates. Nineteen isolates including three ATCC S. aureus reference strains were subjected to PCR detection and DNA sequence analysis for norA and mdeA and active efflux detection using modified minimum inhibitory concentration (MIC) assay. From the 19 isolates, 18 isolates harboured the mdeA gene while 16 isolates contained norA gene. DNA sequence analysis reveals 98–100% correlation between the PCR product and the published DNA sequences in GenBank. In addition, 16 isolates exhibited active efflux activity using the ethidium bromide (EtBr)- reserpine combination MIC assay. To our knowledge, this is the first report on the detection of efflux genes and active efflux activity amongst Malaysian clinical isolates of MRSA/MSSA. Detection of active efflux activity may explain the previous report on efflux-mediated drug resistance profile amongst the local clinical isolates. Wiley-Blackwell 2008 Article PeerReviewed Saiful, Azmi Johari and Mohtar, Mastura and Zarizal, Suhaili and Mazurah, Mohamed Isa and Shuhaimi, Mustafa and Ali, Abdul Manaf (2008) Efflux genes and active efflux activity detection in of methicillin-resistant Staphylococcus aureus (MRSA). Journal of Basic Microbiology, 48 (4). pp. 245-251. ISSN 0233-111X Sequence Analysis, DNA - methods. Staphylococcus aurens. Methicillin resistance. DOI: 10.1002/jobm.200700387 English |
| spellingShingle | Sequence Analysis, DNA - methods. Staphylococcus aurens. Methicillin resistance. Saiful, Azmi Johari Mohtar, Mastura Zarizal, Suhaili Mazurah, Mohamed Isa Shuhaimi, Mustafa Ali, Abdul Manaf Efflux genes and active efflux activity detection in of methicillin-resistant Staphylococcus aureus (MRSA). |
| title | Efflux genes and active efflux activity detection in of methicillin-resistant Staphylococcus aureus (MRSA). |
| title_full | Efflux genes and active efflux activity detection in of methicillin-resistant Staphylococcus aureus (MRSA). |
| title_fullStr | Efflux genes and active efflux activity detection in of methicillin-resistant Staphylococcus aureus (MRSA). |
| title_full_unstemmed | Efflux genes and active efflux activity detection in of methicillin-resistant Staphylococcus aureus (MRSA). |
| title_short | Efflux genes and active efflux activity detection in of methicillin-resistant Staphylococcus aureus (MRSA). |
| title_sort | efflux genes and active efflux activity detection in of methicillin-resistant staphylococcus aureus (mrsa). |
| topic | Sequence Analysis, DNA - methods. Staphylococcus aurens. Methicillin resistance. |
| url | http://psasir.upm.edu.my/id/eprint/14444/ http://psasir.upm.edu.my/id/eprint/14444/ |