Detection and quantification of probiotic bacteria using optimized DNA extraction, traditional and real-time PCR methods in complex microbial communities

The aim of this study is to optimize molecular detection and quantification methods of probiotic bacteria in complex microbial communities that have long been difficult for traditional culture-based methods. Traditional and real-time PCR were optimized to detect and quantify Lactobacillus spp. and B...

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Bibliographic Details
Main Authors: Abdulamir, Ahmed Sahib, Yoke, T. S., Nordin, Noordiana, Abu Bakar, Fatimah
Format: Article
Language:English
Published: Academic Journals 2010
Online Access:http://psasir.upm.edu.my/id/eprint/13928/
http://psasir.upm.edu.my/id/eprint/13928/1/13928.pdf