Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin.
Background: Vanillin is responsible for the flavor and smell of vanilla, a widely used flavoring agent. Previous studies showed that vanillin could enhance the repair of mutations and thus function as an anti-mutagen. However, its role in cancer, a disease that is closely related to mutation has n...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13214/ http://psasir.upm.edu.my/id/eprint/13214/1/Apoptosis%20and%20cell%20cycle%20arrest%20of%20human%20colorectal%20cancer%20cell%20line%20HT.pdf |
| _version_ | 1848842051224862720 |
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| author | Ho, Ket Li Saiful Yazan, Latifah Ismail, Norsharina Ismail, Maznah |
| author_facet | Ho, Ket Li Saiful Yazan, Latifah Ismail, Norsharina Ismail, Maznah |
| author_sort | Ho, Ket Li |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background:
Vanillin is responsible for the flavor and smell of vanilla, a widely used flavoring agent. Previous studies showed that vanillin could enhance the repair of mutations and thus function as an anti-mutagen. However, its role in cancer, a disease that is closely related to mutation has not yet been fully elucidated.
Methods:
Hence, this study investigated the cytolytic and cytostatic properties of vanillin against HT-29, a human colorectal cancer cell line. Methods used including cell viability assay, acridine orange (AO)–ethidium bromide (EB) double staining cell morphological analysis, Cell cycle analysis, annexin V–propidium iodide apoptosis test and 5-bromo-2-deoxyuridine (BrdU)-labeling cell proliferation assay.
Results:
Results showed that apoptosis was induced by vanillin and the IC50 for HT-29 and NIH/3T3 normal cell lines were 400 μg/ml and 1000 μg/ml, respectively. Different concentrations of vanillin arrest cell cycle at different checkpoints. 5-Bromo-2-deoxyuridine-labeling cell proliferation assay showed that G0/G1 arrest was achieved at lower concentration of vanillin (200 μg/ml) while cell cycle analysis by flow cytometer showed that G2/M arrest occurs at higher concentration of vanillin (1000 μg/ml).
Conclusion:
Cytolytic and cytostatic effects shown by vanillin showed that it could be a useful colorectal cancer preventive agent. Further in vivo study should be carried out to confirm that similar effects could happen in animals. |
| first_indexed | 2025-11-15T07:52:59Z |
| format | Article |
| id | upm-13214 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:52:59Z |
| publishDate | 2009 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-132142015-09-15T04:01:09Z http://psasir.upm.edu.my/id/eprint/13214/ Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin. Ho, Ket Li Saiful Yazan, Latifah Ismail, Norsharina Ismail, Maznah Background: Vanillin is responsible for the flavor and smell of vanilla, a widely used flavoring agent. Previous studies showed that vanillin could enhance the repair of mutations and thus function as an anti-mutagen. However, its role in cancer, a disease that is closely related to mutation has not yet been fully elucidated. Methods: Hence, this study investigated the cytolytic and cytostatic properties of vanillin against HT-29, a human colorectal cancer cell line. Methods used including cell viability assay, acridine orange (AO)–ethidium bromide (EB) double staining cell morphological analysis, Cell cycle analysis, annexin V–propidium iodide apoptosis test and 5-bromo-2-deoxyuridine (BrdU)-labeling cell proliferation assay. Results: Results showed that apoptosis was induced by vanillin and the IC50 for HT-29 and NIH/3T3 normal cell lines were 400 μg/ml and 1000 μg/ml, respectively. Different concentrations of vanillin arrest cell cycle at different checkpoints. 5-Bromo-2-deoxyuridine-labeling cell proliferation assay showed that G0/G1 arrest was achieved at lower concentration of vanillin (200 μg/ml) while cell cycle analysis by flow cytometer showed that G2/M arrest occurs at higher concentration of vanillin (1000 μg/ml). Conclusion: Cytolytic and cytostatic effects shown by vanillin showed that it could be a useful colorectal cancer preventive agent. Further in vivo study should be carried out to confirm that similar effects could happen in animals. Elsevier 2009-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13214/1/Apoptosis%20and%20cell%20cycle%20arrest%20of%20human%20colorectal%20cancer%20cell%20line%20HT.pdf Ho, Ket Li and Saiful Yazan, Latifah and Ismail, Norsharina and Ismail, Maznah (2009) Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin. Cancer Epidemiology, 33 (2). pp. 155-160. ISSN 1877-7821; ESSN: 1877-783X 10.1016/j.canep.2009.06.003 |
| spellingShingle | Ho, Ket Li Saiful Yazan, Latifah Ismail, Norsharina Ismail, Maznah Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin. |
| title | Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin. |
| title_full | Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin. |
| title_fullStr | Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin. |
| title_full_unstemmed | Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin. |
| title_short | Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin. |
| title_sort | apoptosis and cell cycle arrest of human colorectal cancer cell line ht-29 induced by vanillin. |
| url | http://psasir.upm.edu.my/id/eprint/13214/ http://psasir.upm.edu.my/id/eprint/13214/ http://psasir.upm.edu.my/id/eprint/13214/1/Apoptosis%20and%20cell%20cycle%20arrest%20of%20human%20colorectal%20cancer%20cell%20line%20HT.pdf |