Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372.
Secondary metabolites from actinomycetes especially the genus Streptomyces may be one of the most important sources for novel anticancer agents. A purified fraction from a novel actinomycete strain, Streptomyces sp. H7372, was elucidated in breast cancer cells. We have isolated three purified fracti...
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| Format: | Article |
| Language: | English |
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Spandidos Publications
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/13205/ http://psasir.upm.edu.my/id/eprint/13205/1/Anti-proliferative%20and%20anti-invasive%20properties%20of%20a%20purified%20fraction%20from%20Streptomyces%20sp.%20H7372..pdf |
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| author | Yip, Wai Kien Cheenpracha, Sarot Chang, Leng Chee Ho, Coy Choke Seow, Heng Fong |
| author_facet | Yip, Wai Kien Cheenpracha, Sarot Chang, Leng Chee Ho, Coy Choke Seow, Heng Fong |
| author_sort | Yip, Wai Kien |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Secondary metabolites from actinomycetes especially the genus Streptomyces may be one of the most important sources for novel anticancer agents. A purified fraction from a novel actinomycete strain, Streptomyces sp. H7372, was elucidated in breast cancer cells. We have isolated three purified fractions from a novel strain, Streptomyces sp. H7372. One of the fractions, designated as 31-2, exhibited the strongest growth-inhibitory effect and thereby was selected for further studies. 31-2 exerted a growth-inhibitory effect on a panel of 15 human cancer and 2 non-malignant cell lines. In MCF-7 and MDA-MB-231 breast cancer cells, 31-2 induced a cytostatic (anti-proliferative) effect without causing cytotoxicity (cell death). Our data suggest that the cytostasis resulted from cell cycle arrest at the G1 phase in MCF-7 cells and at the S phase in MDA-MB-231 cells. Western blot analysis demonstrated a modulation of phosphorylation of the Rb and CDC2 proteins and of CDK4, cyclin D1 and cyclin D3 in the 31-2-treated breast cancer cell lines. The protein levels of CDK2, CDK6, and PCNA were not affected by 31-2 treatment. 31-2 also exhibited an anti-invasive effect in MDA-MB-231 cells. However, this effect is not attributed to the modulation of proteolytic activity in MDA-MB-231 cells as the enzymatic degradation of type IV collagen was not affected by 31-2. The 31-2 is a potent cytostatic and anti-invasive agent and modulates the cell cycle pathway. Together, these results will have important implications in searching for novel approaches to treat cancer. |
| first_indexed | 2025-11-15T07:52:57Z |
| format | Article |
| id | upm-13205 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:52:57Z |
| publishDate | 2010 |
| publisher | Spandidos Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-132052015-10-30T00:34:39Z http://psasir.upm.edu.my/id/eprint/13205/ Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372. Yip, Wai Kien Cheenpracha, Sarot Chang, Leng Chee Ho, Coy Choke Seow, Heng Fong Secondary metabolites from actinomycetes especially the genus Streptomyces may be one of the most important sources for novel anticancer agents. A purified fraction from a novel actinomycete strain, Streptomyces sp. H7372, was elucidated in breast cancer cells. We have isolated three purified fractions from a novel strain, Streptomyces sp. H7372. One of the fractions, designated as 31-2, exhibited the strongest growth-inhibitory effect and thereby was selected for further studies. 31-2 exerted a growth-inhibitory effect on a panel of 15 human cancer and 2 non-malignant cell lines. In MCF-7 and MDA-MB-231 breast cancer cells, 31-2 induced a cytostatic (anti-proliferative) effect without causing cytotoxicity (cell death). Our data suggest that the cytostasis resulted from cell cycle arrest at the G1 phase in MCF-7 cells and at the S phase in MDA-MB-231 cells. Western blot analysis demonstrated a modulation of phosphorylation of the Rb and CDC2 proteins and of CDK4, cyclin D1 and cyclin D3 in the 31-2-treated breast cancer cell lines. The protein levels of CDK2, CDK6, and PCNA were not affected by 31-2 treatment. 31-2 also exhibited an anti-invasive effect in MDA-MB-231 cells. However, this effect is not attributed to the modulation of proteolytic activity in MDA-MB-231 cells as the enzymatic degradation of type IV collagen was not affected by 31-2. The 31-2 is a potent cytostatic and anti-invasive agent and modulates the cell cycle pathway. Together, these results will have important implications in searching for novel approaches to treat cancer. Spandidos Publications 2010-11 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13205/1/Anti-proliferative%20and%20anti-invasive%20properties%20of%20a%20purified%20fraction%20from%20Streptomyces%20sp.%20H7372..pdf Yip, Wai Kien and Cheenpracha, Sarot and Chang, Leng Chee and Ho, Coy Choke and Seow, Heng Fong (2010) Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372. International Journal of Oncology, 37 (5). pp. 1229-1241. ISSN 1019-6439; ESSN: 1791-2423 http://www.spandidos-publications.com/ijo/37/5/1229 |
| spellingShingle | Yip, Wai Kien Cheenpracha, Sarot Chang, Leng Chee Ho, Coy Choke Seow, Heng Fong Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372. |
| title | Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372. |
| title_full | Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372. |
| title_fullStr | Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372. |
| title_full_unstemmed | Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372. |
| title_short | Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372. |
| title_sort | anti-proliferative and anti-invasive properties of a purified fraction from streptomyces sp. h7372. |
| url | http://psasir.upm.edu.my/id/eprint/13205/ http://psasir.upm.edu.my/id/eprint/13205/ http://psasir.upm.edu.my/id/eprint/13205/1/Anti-proliferative%20and%20anti-invasive%20properties%20of%20a%20purified%20fraction%20from%20Streptomyces%20sp.%20H7372..pdf |