A low molecular weight lipopolysaccharide antigen preparation reactive to acute leptospirosis heterologous sera.
There is a need for identification of new infection markers against common Leptospira isolates in Malaysia. To achieve this goal, seven-day-old cultures of Leptospira interrogans serogroup Icterohemorrhagiae (L44) and Leptospira interrogans serogroup Javanica (L55) were used for antigen preparation...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Malaysian Society of Parasitology and Tropical Medicine
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/12714/ http://psasir.upm.edu.my/id/eprint/12714/1/A%20low%20molecular%20weight%20lipopolysaccharide%20antigen%20preparation%20reactive%20to%20acute%20leptospirosis%20heterologous%20sera.pdf |
| _version_ | 1848841910546857984 |
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| author | M., Riazi Bahaman, Abdul Rani Abdullah, Fairuz F.Z., Zainul |
| author_facet | M., Riazi Bahaman, Abdul Rani Abdullah, Fairuz F.Z., Zainul |
| author_sort | M., Riazi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | There is a need for identification of new infection markers against common Leptospira isolates in Malaysia. To achieve this goal, seven-day-old cultures of Leptospira interrogans serogroup Icterohemorrhagiae (L44) and Leptospira interrogans serogroup Javanica (L55) were used for antigen preparation by sequential extraction method using 40 mM Tris, 8M Urea and 2M thiourea. Immunoblot analysis of the antigens were performed using serum samples from 46 local patients with confirmed acute leptospirosis, 28 patients with other infections and 14 healthy controls. The patients serum samples used in this study contained heterologous antibody against a number of different Leptospira serovars. A strong IgM reactivity to a broad diffuse band of 10-15 kDa was observed. Combining results using L44 and L55 antigens showed sensitivity of 80.4% and specificity of 95.2% for detection of leptospirosis. Proteinase K and periodate treatment indicated that the band is likely to be lipopolysaccharide (LPS) in nature. This study showed that the 10-15 kDa antigen could potentially be useful for serodiagnosis of acute leptospirosis in Malaysia. |
| first_indexed | 2025-11-15T07:50:45Z |
| format | Article |
| id | upm-12714 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:50:45Z |
| publishDate | 2010 |
| publisher | Malaysian Society of Parasitology and Tropical Medicine |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-127142015-10-22T00:26:33Z http://psasir.upm.edu.my/id/eprint/12714/ A low molecular weight lipopolysaccharide antigen preparation reactive to acute leptospirosis heterologous sera. M., Riazi Bahaman, Abdul Rani Abdullah, Fairuz F.Z., Zainul There is a need for identification of new infection markers against common Leptospira isolates in Malaysia. To achieve this goal, seven-day-old cultures of Leptospira interrogans serogroup Icterohemorrhagiae (L44) and Leptospira interrogans serogroup Javanica (L55) were used for antigen preparation by sequential extraction method using 40 mM Tris, 8M Urea and 2M thiourea. Immunoblot analysis of the antigens were performed using serum samples from 46 local patients with confirmed acute leptospirosis, 28 patients with other infections and 14 healthy controls. The patients serum samples used in this study contained heterologous antibody against a number of different Leptospira serovars. A strong IgM reactivity to a broad diffuse band of 10-15 kDa was observed. Combining results using L44 and L55 antigens showed sensitivity of 80.4% and specificity of 95.2% for detection of leptospirosis. Proteinase K and periodate treatment indicated that the band is likely to be lipopolysaccharide (LPS) in nature. This study showed that the 10-15 kDa antigen could potentially be useful for serodiagnosis of acute leptospirosis in Malaysia. Malaysian Society of Parasitology and Tropical Medicine 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12714/1/A%20low%20molecular%20weight%20lipopolysaccharide%20antigen%20preparation%20reactive%20to%20acute%20leptospirosis%20heterologous%20sera.pdf M., Riazi and Bahaman, Abdul Rani and Abdullah, Fairuz and F.Z., Zainul (2010) A low molecular weight lipopolysaccharide antigen preparation reactive to acute leptospirosis heterologous sera. Tropical Biomedicine, 27 (2). pp. 241-253. ISSN 0127-5720 English |
| spellingShingle | M., Riazi Bahaman, Abdul Rani Abdullah, Fairuz F.Z., Zainul A low molecular weight lipopolysaccharide antigen preparation reactive to acute leptospirosis heterologous sera. |
| title | A low molecular weight lipopolysaccharide antigen preparation reactive to acute leptospirosis heterologous sera. |
| title_full | A low molecular weight lipopolysaccharide antigen preparation reactive to acute leptospirosis heterologous sera. |
| title_fullStr | A low molecular weight lipopolysaccharide antigen preparation reactive to acute leptospirosis heterologous sera. |
| title_full_unstemmed | A low molecular weight lipopolysaccharide antigen preparation reactive to acute leptospirosis heterologous sera. |
| title_short | A low molecular weight lipopolysaccharide antigen preparation reactive to acute leptospirosis heterologous sera. |
| title_sort | low molecular weight lipopolysaccharide antigen preparation reactive to acute leptospirosis heterologous sera. |
| url | http://psasir.upm.edu.my/id/eprint/12714/ http://psasir.upm.edu.my/id/eprint/12714/1/A%20low%20molecular%20weight%20lipopolysaccharide%20antigen%20preparation%20reactive%20to%20acute%20leptospirosis%20heterologous%20sera.pdf |