In vitro evaluation of scavenging and anti-adipogenic activity of Momordica cochinchinensis (Lour.). Spreng fruit extracts on 3T3-L1 adipocytes
Gac fruit, scientifically known as Momordica cochinchinensis (Lour) Spreng, is rich in potent bioactive compounds, particularly carotenoids such as β-carotene, lycopene, and lutein. This study investigated the effects of gac fruit extract fractions (peel, pulp, and aril) on the scavenging, cytotoxic...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society for Biochemistry & Molecular Biology
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/121149/ http://psasir.upm.edu.my/id/eprint/121149/1/121149.pdf |
| _version_ | 1848868305792663552 |
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| author | Abdul Rahman, Mohd Nazri Ismail, Amin Azlan, Azrina Abdul Aziz, Ahmad Fazli Muhammad, Nor Hayati |
| author_facet | Abdul Rahman, Mohd Nazri Ismail, Amin Azlan, Azrina Abdul Aziz, Ahmad Fazli Muhammad, Nor Hayati |
| author_sort | Abdul Rahman, Mohd Nazri |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Gac fruit, scientifically known as Momordica cochinchinensis (Lour) Spreng, is rich in potent bioactive compounds, particularly carotenoids such as β-carotene, lycopene, and lutein. This study investigated the effects of gac fruit extract fractions (peel, pulp, and aril) on the scavenging, cytotoxic, and anti-adipogenic activities in 3T3-L1 adipocytes. The study assessed the DPPH radical scavenging activity of gac extracts through serial dilution at a concentration of 1000 μg/mL. The viability of 3T3-L1 preadipocytes was measured using the MTT assay. Differentiated adipocytes were treated with gac extracts at concentrations of 75, 150, and 300 μg/mL for 7 days. The impact on lipid accumulation and adipogenesis inhibition was determined through Oil Red O staining and triglyceride content analysis. The IC50 values for DPPH radical scavenging were 573.40 μg/mL for peel, 525.46 μg/mL for pulp, and 817.33 μg/mL for aril extracts. No toxicity was observed in 3T3-L1 cells at concentrations up to 200 μg/mL. At 200 μg/mL, gac extracts reduced 3T3-L1 cell viability while promoting growth and proliferation. Treatment with gac extracts significantly reduced lipid accumulation and inhibited 3T3-L1 cell differentiation in a dose-dependent manner. Among the gac extract fractions, pulp notably decreased intracellular triglyceride content in adipocytes, surpassing aril and peel extracts. In conclusion, gac fruit extract fractions (peel, pulp, and aril) effectively inhibited adipogenesis in 3T3-L1 adipocytes, as evidenced by reduced lipid accumulation, triglyceride content, and cell viability. These findings unveil valuable insights into bioactive compounds from Momordica cochinchinensis and their potential for addressing obesity prevention and treatment. |
| first_indexed | 2025-11-15T14:50:17Z |
| format | Article |
| id | upm-121149 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:50:17Z |
| publishDate | 2024 |
| publisher | Malaysian Society for Biochemistry & Molecular Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1211492025-10-28T06:48:03Z http://psasir.upm.edu.my/id/eprint/121149/ In vitro evaluation of scavenging and anti-adipogenic activity of Momordica cochinchinensis (Lour.). Spreng fruit extracts on 3T3-L1 adipocytes Abdul Rahman, Mohd Nazri Ismail, Amin Azlan, Azrina Abdul Aziz, Ahmad Fazli Muhammad, Nor Hayati Gac fruit, scientifically known as Momordica cochinchinensis (Lour) Spreng, is rich in potent bioactive compounds, particularly carotenoids such as β-carotene, lycopene, and lutein. This study investigated the effects of gac fruit extract fractions (peel, pulp, and aril) on the scavenging, cytotoxic, and anti-adipogenic activities in 3T3-L1 adipocytes. The study assessed the DPPH radical scavenging activity of gac extracts through serial dilution at a concentration of 1000 μg/mL. The viability of 3T3-L1 preadipocytes was measured using the MTT assay. Differentiated adipocytes were treated with gac extracts at concentrations of 75, 150, and 300 μg/mL for 7 days. The impact on lipid accumulation and adipogenesis inhibition was determined through Oil Red O staining and triglyceride content analysis. The IC50 values for DPPH radical scavenging were 573.40 μg/mL for peel, 525.46 μg/mL for pulp, and 817.33 μg/mL for aril extracts. No toxicity was observed in 3T3-L1 cells at concentrations up to 200 μg/mL. At 200 μg/mL, gac extracts reduced 3T3-L1 cell viability while promoting growth and proliferation. Treatment with gac extracts significantly reduced lipid accumulation and inhibited 3T3-L1 cell differentiation in a dose-dependent manner. Among the gac extract fractions, pulp notably decreased intracellular triglyceride content in adipocytes, surpassing aril and peel extracts. In conclusion, gac fruit extract fractions (peel, pulp, and aril) effectively inhibited adipogenesis in 3T3-L1 adipocytes, as evidenced by reduced lipid accumulation, triglyceride content, and cell viability. These findings unveil valuable insights into bioactive compounds from Momordica cochinchinensis and their potential for addressing obesity prevention and treatment. Malaysian Society for Biochemistry & Molecular Biology 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/121149/1/121149.pdf Abdul Rahman, Mohd Nazri and Ismail, Amin and Azlan, Azrina and Abdul Aziz, Ahmad Fazli and Muhammad, Nor Hayati (2024) In vitro evaluation of scavenging and anti-adipogenic activity of Momordica cochinchinensis (Lour.). Spreng fruit extracts on 3T3-L1 adipocytes. Malaysian Journal of Biochemistry and Molecular Biology, 1. pp. 77-99. ISSN 1511-2616; eISSN: 2600-9005 https://drive.google.com/file/d/1B9yMig20AKPV3TMZf5TibULQ1gPuBJgr/view?usp=share_link |
| spellingShingle | Abdul Rahman, Mohd Nazri Ismail, Amin Azlan, Azrina Abdul Aziz, Ahmad Fazli Muhammad, Nor Hayati In vitro evaluation of scavenging and anti-adipogenic activity of Momordica cochinchinensis (Lour.). Spreng fruit extracts on 3T3-L1 adipocytes |
| title | In vitro evaluation of scavenging and anti-adipogenic activity of Momordica cochinchinensis (Lour.). Spreng fruit extracts on 3T3-L1 adipocytes |
| title_full | In vitro evaluation of scavenging and anti-adipogenic activity of Momordica cochinchinensis (Lour.). Spreng fruit extracts on 3T3-L1 adipocytes |
| title_fullStr | In vitro evaluation of scavenging and anti-adipogenic activity of Momordica cochinchinensis (Lour.). Spreng fruit extracts on 3T3-L1 adipocytes |
| title_full_unstemmed | In vitro evaluation of scavenging and anti-adipogenic activity of Momordica cochinchinensis (Lour.). Spreng fruit extracts on 3T3-L1 adipocytes |
| title_short | In vitro evaluation of scavenging and anti-adipogenic activity of Momordica cochinchinensis (Lour.). Spreng fruit extracts on 3T3-L1 adipocytes |
| title_sort | in vitro evaluation of scavenging and anti-adipogenic activity of momordica cochinchinensis (lour.). spreng fruit extracts on 3t3-l1 adipocytes |
| url | http://psasir.upm.edu.my/id/eprint/121149/ http://psasir.upm.edu.my/id/eprint/121149/ http://psasir.upm.edu.my/id/eprint/121149/1/121149.pdf |