Identification of the anti-epileptic effect of speciogynine through molecular network-aided metabolite profiling of M. speciosa alkaloids using zebrafish
Mitragyna speciosa is a native plant to Southeast Asian countries, known its medicinal use as an analgesic and stimulant. Apart from the indole-type alkaloids, which have been shown to be responsible for its pharmacological properties, there is still limited information on other classes of compounds...
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| Format: | Article |
| Language: | English |
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Elsevier B.V.
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/120720/ http://psasir.upm.edu.my/id/eprint/120720/1/120720.pdf |
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| author | Garba, Suleiman Abubakar Swain, Puspanjali Shaari, Khozirah Afzan, Adlin Lee, Soo Yee Kim, Cheol-Hee Mohd Faudzi, Siti Munirah |
| author_facet | Garba, Suleiman Abubakar Swain, Puspanjali Shaari, Khozirah Afzan, Adlin Lee, Soo Yee Kim, Cheol-Hee Mohd Faudzi, Siti Munirah |
| author_sort | Garba, Suleiman Abubakar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Mitragyna speciosa is a native plant to Southeast Asian countries, known its medicinal use as an analgesic and stimulant. Apart from the indole-type alkaloids, which have been shown to be responsible for its pharmacological properties, there is still limited information on other classes of compounds present in various parts of the plant. In an effort to fill this knowledge gap, the present study applied tandem liquid chromatography-mass spectrometry (LC-MS/MS)-based molecular networking to obtain more information on the chemodiversity of M. speciosa. Analysis of the generated molecular map obtained from methanol extracts of leaves (green- and red-veined), stem bark, stem tissue and fruits, revealed the putative presence of not only the characteristic indole-type alkaloids, but also flavoalkaloids, iridoids, triterpenoid saponins, glycoside derivatives, phenolic acids, flavonoids, and polyphenols, pervading all different parts of the plant. The class of flavoalkaloids is reported herein as new for M. speciosa. Continuing our interest in the biological properties of the chemical constituents of the plant, two minor indole alkaloids, speciogynine and isopaynantheine, purified from the leaf extract were evaluated for their toxicity and antiepileptic potential in zebrafish animal model. Interestingly, speciogynine exhibited a significant dose-dependent effect in reducing hyperactive movements in a zebrafish epilepsy model with mild toxic effects, indicating that it could be a promising anti-epileptic lead compound for further exploration. |
| first_indexed | 2025-11-15T14:48:57Z |
| format | Article |
| id | upm-120720 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:48:57Z |
| publishDate | 2025 |
| publisher | Elsevier B.V. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1207202025-10-08T08:24:18Z http://psasir.upm.edu.my/id/eprint/120720/ Identification of the anti-epileptic effect of speciogynine through molecular network-aided metabolite profiling of M. speciosa alkaloids using zebrafish Garba, Suleiman Abubakar Swain, Puspanjali Shaari, Khozirah Afzan, Adlin Lee, Soo Yee Kim, Cheol-Hee Mohd Faudzi, Siti Munirah Mitragyna speciosa is a native plant to Southeast Asian countries, known its medicinal use as an analgesic and stimulant. Apart from the indole-type alkaloids, which have been shown to be responsible for its pharmacological properties, there is still limited information on other classes of compounds present in various parts of the plant. In an effort to fill this knowledge gap, the present study applied tandem liquid chromatography-mass spectrometry (LC-MS/MS)-based molecular networking to obtain more information on the chemodiversity of M. speciosa. Analysis of the generated molecular map obtained from methanol extracts of leaves (green- and red-veined), stem bark, stem tissue and fruits, revealed the putative presence of not only the characteristic indole-type alkaloids, but also flavoalkaloids, iridoids, triterpenoid saponins, glycoside derivatives, phenolic acids, flavonoids, and polyphenols, pervading all different parts of the plant. The class of flavoalkaloids is reported herein as new for M. speciosa. Continuing our interest in the biological properties of the chemical constituents of the plant, two minor indole alkaloids, speciogynine and isopaynantheine, purified from the leaf extract were evaluated for their toxicity and antiepileptic potential in zebrafish animal model. Interestingly, speciogynine exhibited a significant dose-dependent effect in reducing hyperactive movements in a zebrafish epilepsy model with mild toxic effects, indicating that it could be a promising anti-epileptic lead compound for further exploration. Elsevier B.V. 2025 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/120720/1/120720.pdf Garba, Suleiman Abubakar and Swain, Puspanjali and Shaari, Khozirah and Afzan, Adlin and Lee, Soo Yee and Kim, Cheol-Hee and Mohd Faudzi, Siti Munirah (2025) Identification of the anti-epileptic effect of speciogynine through molecular network-aided metabolite profiling of M. speciosa alkaloids using zebrafish. Fitoterapia, 184. art. no. 106667. pp. 1-17. ISSN 0367-326X; eISSN: 1873-6971 https://www.sciencedirect.com/science/article/pii/S0367326X25002928?via%3Dihub 10.1016/j.fitote.2025.106667 |
| spellingShingle | Garba, Suleiman Abubakar Swain, Puspanjali Shaari, Khozirah Afzan, Adlin Lee, Soo Yee Kim, Cheol-Hee Mohd Faudzi, Siti Munirah Identification of the anti-epileptic effect of speciogynine through molecular network-aided metabolite profiling of M. speciosa alkaloids using zebrafish |
| title | Identification of the anti-epileptic effect of speciogynine through molecular network-aided metabolite profiling of M. speciosa alkaloids using zebrafish |
| title_full | Identification of the anti-epileptic effect of speciogynine through molecular network-aided metabolite profiling of M. speciosa alkaloids using zebrafish |
| title_fullStr | Identification of the anti-epileptic effect of speciogynine through molecular network-aided metabolite profiling of M. speciosa alkaloids using zebrafish |
| title_full_unstemmed | Identification of the anti-epileptic effect of speciogynine through molecular network-aided metabolite profiling of M. speciosa alkaloids using zebrafish |
| title_short | Identification of the anti-epileptic effect of speciogynine through molecular network-aided metabolite profiling of M. speciosa alkaloids using zebrafish |
| title_sort | identification of the anti-epileptic effect of speciogynine through molecular network-aided metabolite profiling of m. speciosa alkaloids using zebrafish |
| url | http://psasir.upm.edu.my/id/eprint/120720/ http://psasir.upm.edu.my/id/eprint/120720/ http://psasir.upm.edu.my/id/eprint/120720/ http://psasir.upm.edu.my/id/eprint/120720/1/120720.pdf |