Comparative evaluation of hydrolyzed vs. un-hydrolyzed edible bird's nest across raw unclean and by-product grades

This study examines the impact of enzymatic hydrolysis on the nutritional properties and safety of edible bird's nest (EBN), particularly focusing on raw unclean (RUC) EBN and by-product EBN. Employing a comparative approach, the research analyzes four samples: two non-hydrolyzed (cup-shaped RU...

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Main Authors: Yeo, Bee Hui, Tang, Teck Kim, Wong, Shew Fung, Tan, Chin Ping, Rukayadi, Yaya, Lai, Oi Ming
Format: Article
Published: Khon Kaen University,Research and Technology Transfer Affairs Division 2025
Online Access:http://psasir.upm.edu.my/id/eprint/120606/
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author Yeo, Bee Hui
Tang, Teck Kim
Wong, Shew Fung
Tan, Chin Ping
Rukayadi, Yaya
Lai, Oi Ming
author_facet Yeo, Bee Hui
Tang, Teck Kim
Wong, Shew Fung
Tan, Chin Ping
Rukayadi, Yaya
Lai, Oi Ming
author_sort Yeo, Bee Hui
building UPM Institutional Repository
collection Online Access
description This study examines the impact of enzymatic hydrolysis on the nutritional properties and safety of edible bird's nest (EBN), particularly focusing on raw unclean (RUC) EBN and by-product EBN. Employing a comparative approach, the research analyzes four samples: two non-hydrolyzed (cup-shaped RUC EBN [EBNc] and by-product of EBN [EBNcp]), and two enzymatically digested (hydrolysate from EBNc [EBNch] and EBNcp [EBNcph]) using bromelain. Nutritional evaluation included measuring sialic acid content, antioxidant activity via DPPH radical scavenging, and amino acid profiling. Results indicated a significant increase in sialic acid content in the hydrolyzed samples (13.97% in EBNch and 14.36% in EBNcph) compared to non-hydrolyzed samples (12.1% in EBNc and 12.3% in EBNcp). Despite a slight reduction in antioxidant activity in hydrolyzed EBNs, they retained substantial antioxidant properties. Amino acid analysis showed the presence of leucine only in hydrolyzed samples, while isoleucine and phenylalanine were exclusive to non-hydrolyzed EBNs. Safety assessments confirmed the absence of heavy metals (arsenic, lead, mercury, cadmium, antimony) in all hydrolysates. Enzymatic hydrolysis significantly enhanced sialic acid content and specific amino acids, maintaining safety standards and proving beneficial for processing heavy feather RUC EBN and by-product EBN, thus improving product recovery and generating valuable by-products. These findings highlight the potential of enzymatic hydrolysis in enhancing the nutritional value and safety of EBN, underscoring its commercial viability.
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institution Universiti Putra Malaysia
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last_indexed 2025-11-15T14:48:46Z
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publisher Khon Kaen University,Research and Technology Transfer Affairs Division
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spelling upm-1206062025-10-07T00:32:17Z http://psasir.upm.edu.my/id/eprint/120606/ Comparative evaluation of hydrolyzed vs. un-hydrolyzed edible bird's nest across raw unclean and by-product grades Yeo, Bee Hui Tang, Teck Kim Wong, Shew Fung Tan, Chin Ping Rukayadi, Yaya Lai, Oi Ming This study examines the impact of enzymatic hydrolysis on the nutritional properties and safety of edible bird's nest (EBN), particularly focusing on raw unclean (RUC) EBN and by-product EBN. Employing a comparative approach, the research analyzes four samples: two non-hydrolyzed (cup-shaped RUC EBN [EBNc] and by-product of EBN [EBNcp]), and two enzymatically digested (hydrolysate from EBNc [EBNch] and EBNcp [EBNcph]) using bromelain. Nutritional evaluation included measuring sialic acid content, antioxidant activity via DPPH radical scavenging, and amino acid profiling. Results indicated a significant increase in sialic acid content in the hydrolyzed samples (13.97% in EBNch and 14.36% in EBNcph) compared to non-hydrolyzed samples (12.1% in EBNc and 12.3% in EBNcp). Despite a slight reduction in antioxidant activity in hydrolyzed EBNs, they retained substantial antioxidant properties. Amino acid analysis showed the presence of leucine only in hydrolyzed samples, while isoleucine and phenylalanine were exclusive to non-hydrolyzed EBNs. Safety assessments confirmed the absence of heavy metals (arsenic, lead, mercury, cadmium, antimony) in all hydrolysates. Enzymatic hydrolysis significantly enhanced sialic acid content and specific amino acids, maintaining safety standards and proving beneficial for processing heavy feather RUC EBN and by-product EBN, thus improving product recovery and generating valuable by-products. These findings highlight the potential of enzymatic hydrolysis in enhancing the nutritional value and safety of EBN, underscoring its commercial viability. Khon Kaen University,Research and Technology Transfer Affairs Division 2025 Article PeerReviewed Yeo, Bee Hui and Tang, Teck Kim and Wong, Shew Fung and Tan, Chin Ping and Rukayadi, Yaya and Lai, Oi Ming (2025) Comparative evaluation of hydrolyzed vs. un-hydrolyzed edible bird's nest across raw unclean and by-product grades. Asia-Pacific Journal of Science and Technology, 30 (4). ISSN 2539-6293; eISSN: 2539-6293 https://doi.nrct.go.th//ListDoi/listDetail?Resolve_DOI=10.14456/apst.2025.65 10.14456/apst.2025.65
spellingShingle Yeo, Bee Hui
Tang, Teck Kim
Wong, Shew Fung
Tan, Chin Ping
Rukayadi, Yaya
Lai, Oi Ming
Comparative evaluation of hydrolyzed vs. un-hydrolyzed edible bird's nest across raw unclean and by-product grades
title Comparative evaluation of hydrolyzed vs. un-hydrolyzed edible bird's nest across raw unclean and by-product grades
title_full Comparative evaluation of hydrolyzed vs. un-hydrolyzed edible bird's nest across raw unclean and by-product grades
title_fullStr Comparative evaluation of hydrolyzed vs. un-hydrolyzed edible bird's nest across raw unclean and by-product grades
title_full_unstemmed Comparative evaluation of hydrolyzed vs. un-hydrolyzed edible bird's nest across raw unclean and by-product grades
title_short Comparative evaluation of hydrolyzed vs. un-hydrolyzed edible bird's nest across raw unclean and by-product grades
title_sort comparative evaluation of hydrolyzed vs. un-hydrolyzed edible bird's nest across raw unclean and by-product grades
url http://psasir.upm.edu.my/id/eprint/120606/
http://psasir.upm.edu.my/id/eprint/120606/
http://psasir.upm.edu.my/id/eprint/120606/